『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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○種別 (必須): □学術論文 (審査論文) [継承]
○言語 (必須): □英語 [継承]
○招待 (推奨):
○審査 (推奨):
○カテゴリ (推奨):
○共著種別 (推奨):
○学究種別 (推奨):
○組織 (推奨):
○著者 (必須): 1. (英) Kamei Reiko (日) (読)
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2. (英) Kitagawa Yoshinori (日) (読)
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3. (英) Kadokura Michinori (日) (読)
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4. (英) Hattori Fumiaki (日) (読)
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5. (英) Hazeki Osamu (日) (読)
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6.蛯名 洋介
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7. (英) Nishihara Tatsuro (日) (読)
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8. (英) Oikawa Shinzo (日) (読)
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○題名 (必須): □(英) Shikonin stimulates glucose uptake in 3T3-L1 adipocytes via an insulin-independent tyrosine kinase pathway.  (日)    [継承]
○副題 (任意):
○要約 (任意): □(英) Type 2 diabetes is due to defects in both insulin action and secretion. In an attempt to discover small molecules that stimulate glucose uptake, similar to insulin, a cell-based glucose uptake screening assay was performed using 3T3-L1 adipocytes. Shikonin, a substance originally isolated from the root of the Chinese plant that has been used as an ointment for wound healing, was thus identified. Shikonin stimulated glucose uptake and potentiated insulin-stimulated glucose uptake in a concentration-dependent manner in 3T3-L1 adipocytes. Stimulation of glucose uptake was also observed in rat primary adipocytes and cardiomyocytes. Like insulin, shikonin-stimulated glucose uptake was inhibited by genistein, a tyrosine kinase inhibitor, and enhanced by vanadate, a tyrosine phosphatase inhibitor. However, in contrast to insulin, shikonin-stimulated glucose uptake was not strongly inhibited by wortmannin, a specific inhibitor of phosphatidylinositol 3-kinase (PI3K). In vitro phosphorylation analyses revealed that shikonin did not induce tyrosine phosphorylation of the insulin receptor, but significantly induced both Thr-308 and Ser-473 phosphorylation of Akt. Our results suggest that in 3T3-L1 adipocytes, shikonin action is not mediated primarily via the insulin receptor/PI3K pathway, but rather via another distinct tyrosine kinase-dependent pathway leading to glucose uptake involving Akt phosphorylation.  (日)    [継承]
○キーワード (推奨): 1. (英) 3T3 Cells (日) (読) [継承]
2. (英) Adipocytes (日) (読) [継承]
3. (英) Androstadienes (日) (読) [継承]
4. (英) Animals (日) (読) [継承]
5. (英) Anti-Inflammatory Agents, Non-Steroidal (日) (読) [継承]
6. (英) Biological Transport (日) (読) [継承]
7. (英) Drugs, Chinese Herbal (日) (読) [継承]
8. (英) Enzyme Inhibitors (日) (読) [継承]
9. (英) Genistein (日) (読) [継承]
10.グルコース (glucose) [継承]
11. (英) Glucose Transporter Type 4 (日) (読) [継承]
12. (英) Humans (日) (読) [継承]
13.インスリン (insulin) [継承]
14. (英) Male (日) (読) [継承]
15. (英) Medicine, Chinese Traditional (日) (読) [継承]
16. (英) Mice (日) (読) [継承]
17.分子構造 (molecular structure) [継承]
18. (英) Monosaccharide Transport Proteins (日) (読) [継承]
19. (英) Muscle Proteins (日) (読) [継承]
20.心筋 (myocardium) [継承]
21. (英) Naphthoquinones (日) (読) [継承]
22.リン酸化 (phosphorylation) [継承]
23. (英) Protein-Serine-Threonine Kinases (日) (読) [継承]
24. (英) Protein-Tyrosine Kinases (日) (読) [継承]
25. (英) Proto-Oncogene Proteins (日) (読) [継承]
26. (英) Proto-Oncogene Proteins c-akt (日) (読) [継承]
27. (英) Rats (日) (読) [継承]
28. (英) Rats, Sprague-Dawley (日) (読) [継承]
29. (英) Receptor, Insulin (日) (読) [継承]
30.シグナル伝達 (signal transduction) [継承]
31. (英) Vanadates (日) (読) [継承]
○発行所 (推奨):
○誌名 (必須): □Biochemical and Biophysical Research Communications ([Elsevier])
(pISSN: 0006-291X, eISSN: 1090-2104)

○ISSN (任意): □0006-291X
ISSN: 0006-291X (pISSN: 0006-291X, eISSN: 1090-2104)
Title: Biochemical and biophysical research communications
Title(ISO): Biochem Biophys Res Commun
Publisher: Elsevier B.V.
 (NLM Catalog)   (Scopus)   (CrossRef)  (Scopus information is found. [need login])
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○巻 (必須): □292 [継承]
○号 (必須): □3 [継承]
○頁 (必須): □624 651 [継承]
○都市 (任意):
○年月日 (必須): □西暦 2002年 4月 5日 (平成 14年 4月 5日) [継承]
○URL (任意):
○DOI (任意): □10.1006/bbrc.2002.6714    (→Scopusで検索) [継承]
○PMID (任意): □11922615    (→Scopusで検索) [継承]
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○備考 (任意): 1.(英) Article.Affiliation: Suntory Biomedical Research Limited, 1-1-1 Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka 618-8503, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Reiko Kamei, Yoshinori Kitagawa, Michinori Kadokura, Fumiaki Hattori, Osamu Hazeki, Yousuke Ebina, Tatsuro Nishihara and Shinzo Oikawa : Shikonin stimulates glucose uptake in 3T3-L1 adipocytes via an insulin-independent tyrosine kinase pathway., Biochemical and Biophysical Research Communications, 292, 3, 624-651, 2002.
欧文冊子 ● Reiko Kamei, Yoshinori Kitagawa, Michinori Kadokura, Fumiaki Hattori, Osamu Hazeki, Yousuke Ebina, Tatsuro Nishihara and Shinzo Oikawa : Shikonin stimulates glucose uptake in 3T3-L1 adipocytes via an insulin-independent tyrosine kinase pathway., Biochemical and Biophysical Research Communications, 292, 3, 624-651, 2002.

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