| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): | □ | Peer Review
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| ○カテゴリ (推奨): | □ | 研究
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| ○共著種別 (推奨): | □ | 学内共著 (徳島大学内研究者との共同研究 (学外研究者を含まない))
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | 廣島 佑香 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔微生物学])
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| 2. | 木戸 理恵 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯周歯内治療学]/[徳島大学.病院.診療科.歯科.歯周病科(第二保存科)])
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| 3. | 木戸 淳一
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| 4. | 板東 美香 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯周歯内治療学]/[徳島大学.病院.診療科.歯科.歯周病科(第二保存科)])
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| 5. | 吉田 賀弥 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.口腔保健学系.口腔保健支援学]/[徳島大学.歯学部.口腔保健学科.口腔保健支援学講座])
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| 6. | 村上 明一 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔微生物学])
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| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
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| 7. | 篠原 康雄 ([徳島大学.先端酵素学研究所.基幹研究部門])
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| ○題名 (必須): | □ | (英) Synthesis of secretory leukocyte protease inhibitor using cell-free protein synthesis system (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) Secretory leukocyte protease inhibitor (SLPI) functions as a protease inhibitor that modulates excessive proteolysis in the body, exhibits broad-spectrum antimicrobial activity, regulates inflammatory responses, and plays an important role in the innate immunity. The purpose of the study was to artificially synthesize a SLPI, an antimicrobial peptide, and investigate its effect on antimicrobial activity against Porphyromonas gingivalis and interleukin-6 (IL-6) production. SLPI protein with a molecular weight of approximately 13 kDa was artificially synthesized using a cell-free protein synthesis (CFPS) system and investigated by western blotting and enzyme-linked immunosorbent assay (ELISA). Disulfide bond isomerase in the protein synthesis mixture increased the amount of SLPI synthesized. The synthesized SLPI (sSLPI) protein was purified and its antimicrobial activity was investigated based on the growth of Porphyromonas gingivalis and bacterial adhesion to oral epithelial cells. The effect of sSLPI on IL-6 production in human periodontal ligament fibroblasts (HPLFs) was examined by ELISA. Our results showed that sSLPI significantly inhibited the growth of Porphyromonas gingivalis and bacterial adhesion to oral epithelial cells and further inhibited IL-6 production by HPLFs. These results suggested that SLPI artificially synthesized using the CFPS system may play a role in the prevention of periodontal diseases through its antimicrobial and anti-inflammatory effects. (日)
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| ○キーワード (推奨): |
| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Odontology (日本歯科大学歯学会)
(pISSN: 1618-1247, eISSN: 1618-1255)
| ○ISSN (任意): | □ | 1618-1255
ISSN: 1618-1247
(pISSN: 1618-1247, eISSN: 1618-1255) Title: OdontologyTitle(ISO): OdontologyPublisher: Springer (NLM Catalog)
(医中誌Web)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 112
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| ○号 (必須): | □ | 4
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| ○頁 (必須): | □ | 1103 1112
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2024年 2月 初日 (令和 6年 2月 初日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1007/s10266-024-00910-8 (→Scopusで検索)
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| ○PMID (任意): | □ | 38502469 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1007/s10266-024-00910-8 (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) KeywordList.Keyword: Porphyromonas gingivalis (日)
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| 4. | (英) KeywordList.Keyword: Antimicrobial activity (日)
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| 5. | (英) KeywordList.Keyword: Cell-free protein synthesis system (日)
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| 6. | (英) KeywordList.Keyword: Interleukin-6 (日)
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| 7. | (英) KeywordList.Keyword: Secretory leukocyte protease inhibitor (日)
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