『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=388414EID:388414, Map:0, LastModified:2022年8月8日(月) 16:40:25, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[村上 明一], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Jayathilake Chathuni (日) (読)
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2. (英) Kumachi Shigefumi (日) (読)
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[継承]
3. (英) Arai Hidenao (日) (読)
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[継承]
4. (英) Motohashi Maiko (日) (読)
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[継承]
5. (英) Terai Takuya (日) (読)
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[継承]
6.村上 明一 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔微生物学])
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[継承]
7. (英) Nemoto Naoto (日) (読)
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題名 (必須): (英) In vitro selection of anti-gliadin single-domain antibodies from a naïve library for cDNA-display mediated immuno-PCR.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Gluten intolerance, or adverse intestinal reactions to gluten, is a fairly common problem among certain groups of people. Celiac disease is the most severe form of gluten intolerance, which can lead to permanent damage in the digestive system. Since lifelong avoidance of gluten is the only available treatment, development of reliable techniques to identify gluten contamination in food is important. Gliadin, a component of gluten, is known to play a major role in gluten toxicity. In this study, cDNA display method was used to select specific single-domain antibodies against toxic gliadin from an alpaca-derived naïve VHH library. The cDNA display method is a promising in vitro display technique, which uniquely converts an unstable mRNA-protein fusion molecule to a stable mRNA/cDNA-protein fusion molecule using a well-designed puromycin linker. Three candidate VHHs were selected and the affinities of the VHHs were observed by pulldown assay and indirect ELISA method. In addition, a novel cDNA display mediated immuno-PCR method (cD-IPCR) was successfully applied to detect gliadin in food. We believe this work demonstrates the potential application of the cDNA display method in selecting binders against toxic and heterogeneous targets such as gliadin with an immunization-free preparation manner.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Camelids, New World (日) (読) [継承]
3. (英) Celiac Disease (日) (読) [継承]
4. (英) Cloning, Molecular (日) (読) [継承]
5. (英) DNA, Complementary (日) (読) [継承]
6. (英) Edible Grain (日) (読) [継承]
7. (英) Enzyme-Linked Immunosorbent Assay (日) (読) [継承]
8. (英) Escherichia coli (日) (読) [継承]
9. (英) Gene Library (日) (読) [継承]
10. (英) Gliadin (日) (読) [継承]
11. (英) Humans (日) (読) [継承]
12. (英) Immunoglobulin Heavy Chains (日) (読) [継承]
13. (英) Polymerase Chain Reaction (日) (読) [継承]
14. (英) Single-Domain Antibodies (日) (読) [継承]
15. (英) Wheat Hypersensitivity (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Analytical Biochemistry: Methods in the Biological Sciences ([Elsevier Science])
(pISSN: 0003-2697, eISSN: 1096-0309)

ISSN (任意): 1096-0309
ISSN: 0003-2697 (pISSN: 0003-2697, eISSN: 1096-0309)
Title: Analytical biochemistry
Title(ISO): Anal Biochem
Publisher: Academic Press Inc.
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 589 [継承]
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都市 (任意):
年月日 (必須): 西暦 2019年 10月 31日 (令和 元年 10月 31日) [継承]
URL (任意):
DOI (任意): 10.1016/j.ab.2019.113490    (→Scopusで検索) [継承]
PMID (任意): 31678363    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) PublicationType: Journal Article  (日)    [継承]
2.(英) PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Chathuni Jayathilake, Shigefumi Kumachi, Hidenao Arai, Maiko Motohashi, Takuya Terai, Akikazu Murakami and Naoto Nemoto : In vitro selection of anti-gliadin single-domain antibodies from a naïve library for cDNA-display mediated immuno-PCR., Analytical Biochemistry: Methods in the Biological Sciences, Vol.589, (号), (頁), 2019.
欧文冊子 ● Chathuni Jayathilake, Shigefumi Kumachi, Hidenao Arai, Maiko Motohashi, Takuya Terai, Akikazu Murakami and Naoto Nemoto : In vitro selection of anti-gliadin single-domain antibodies from a naïve library for cDNA-display mediated immuno-PCR., Analytical Biochemistry: Methods in the Biological Sciences, Vol.589, (号), (頁), 2019.

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