『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=388410)

EID=388410EID:388410, Map:0, LastModified:2022年8月8日(月) 16:44:05, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[村上 明一], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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カテゴリ (推奨):
共著種別 (推奨):
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組織 (推奨):
著者 (必須): 1. (英) Inoue Akihito (日) (読)
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2. (英) Yasuda Takanobu (日) (読)
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3. (英) Zhu Bo (日) (読)
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4. (英) Kitaguchi Tetsuya (日) (読)
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5.村上 明一 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔微生物学])
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6. (英) Ueda Hiroshi (日) (読)
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題名 (必須): (英) Evaluation and selection of potent fluorescent immunosensors by combining fluorescent peptide and nanobodies displayed on yeast surface.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Quenchbody (Q-body) is a quench-based fluorescent immunosensor labeled with fluorescent dye(s) near the antigen-binding site of an antibody. Q-bodies can detect a range of target molecules rapidly and directly. However, because Q-bodies show different antigen responses depending on the antibody used, time-consuming optimization of the Q-body structure is often necessary, and a high-throughput screening method for discriminating and selecting good Q-bodies is required. Here, we aimed to develop a molecular display method of nanobody-based "mini Q-bodies" by combining yeast surface display and coiled-coil forming E4/K4 peptide-based fluorescence labeling. As a result, the yeast-displayed mini Q-body recognizing the anti-cancer agent methotrexate (MTX) showed significant quenching and MTX-dependent dequenching on cells. To demonstrate the applicability of the developed method to select highly responsive mini Q-bodies, a small nanobody library consisting of 30 variants that recognize human serum albumin was used as a model. The best variant, showing a 2.4-fold signal increase, was obtained through selection by flow cytometry. Furthermore, the same nanobody prepared from Escherichia coli also worked as a mini Q-body after dye labeling. The described approach will be applied to quickly obtain well-behaved Q-bodies and other fluorescent biosensors for various targets through directed evolutionary approaches.  (日)    [継承]
キーワード (推奨): 1. (英) Antibodies (日) (読) [継承]
2. (英) Antigens (日) (読) [継承]
3. (英) Biosensing Techniques (日) (読) [継承]
4. (英) Escherichia coli (日) (読) [継承]
5. (英) Flow Cytometry (日) (読) [継承]
6. (英) Fluorescence (日) (読) [継承]
7. (英) Fluorescent Dyes (日) (読) [継承]
8. (英) Humans (日) (読) [継承]
9. (英) Immune System (日) (読) [継承]
10. (英) Immunoassay (日) (読) [継承]
11. (英) Methotrexate (日) (読) [継承]
12. (英) Peptides (日) (読) [継承]
13. (英) Plasmids (日) (読) [継承]
14. (英) Saccharomyces cerevisiae (日) (読) [継承]
15. (英) Serum Albumin, Human (日) (読) [継承]
16. (英) Single-Domain Antibodies (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Scientific Reports ([Nature Publishing Group])
(eISSN: 2045-2322)

ISSN (任意): 2045-2322
ISSN: 2045-2322 (eISSN: 2045-2322)
Title: Scientific reports
Title(ISO): Sci Rep
Publisher: Nature Portfolio
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 11 [継承]
(必須): 1 [継承]
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年月日 (必須): 西暦 2021年 11月 19日 (令和 3年 11月 19日) [継承]
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DOI (任意): 10.1038/s41598-021-02022-7    (→Scopusで検索) [継承]
PMID (任意): 34799644    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) PublicationType: Journal Article  (日)    [継承]
2.(英) PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Akihito Inoue, Takanobu Yasuda, Bo Zhu, Tetsuya Kitaguchi, Akikazu Murakami and Hiroshi Ueda : Evaluation and selection of potent fluorescent immunosensors by combining fluorescent peptide and nanobodies displayed on yeast surface., Scientific Reports, Vol.11, No.1, (頁), 2021.
欧文冊子 ● Akihito Inoue, Takanobu Yasuda, Bo Zhu, Tetsuya Kitaguchi, Akikazu Murakami and Hiroshi Ueda : Evaluation and selection of potent fluorescent immunosensors by combining fluorescent peptide and nanobodies displayed on yeast surface., Scientific Reports, Vol.11, No.1, (頁), 2021.

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