『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=388409)

EID=388409EID:388409, Map:0, LastModified:2022年8月8日(月) 16:46:38, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[村上 明一], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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著者 (必須): 1. (英) Nishiguchi Akihiro (日) (読)
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2.村上 明一 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔微生物学])
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3. (英) Azuma Takachika (日) (読)
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4. (英) Oda Masayuki (日) (読)
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題名 (必須): (英) A Trade-off Between Thermostability and Binding Affinity of Anti-(4-hydroxy-3-nitrophenyl)Acetyl Antibodies During the Course of Affinity Maturation.  (日)    [継承]
副題 (任意):
要約 (任意): (英)   (日) Somatic hypermutation (SHM) is one of the driving forces that increases antibody (Ab) affinity. We studied the effects of SHM on thermostability and affinity using three single-chain Fv fragments (scFvs) of anti-(4-hydroxy-3-nitrophenyl)acetyl Abs, namely 9TG, 9T7, and E11. 9TG has a germline structure that lacks SHM and is an ancestor of 9T7 with 11 mutations. E11, which has 21 mutations, is a mature Ab and has its own ancestor. The thermostabilities and antigen-Ab interactions were analyzed by circular dichroism (CD), differential scanning calorimetry (DSC), and isothermal titration calorimetry (ITC). Far-UV CD spectra showed that all scFvs were folded into a structure referred to as immunoglobulin-fold and were unfolded by heating at different melting temperatures. Comparison of thermodynamic parameters obtained from DSC and ITC revealed that the magnitude of stabilization free energy at 37 °C was in the order, 9TG > 9T7 > E11, while that of the free energy of interaction with antigen was 9TG < 9T7 < E11, suggesting that Abs make a trade-off between stability and affinity during affinity maturation.   [継承]
キーワード (推奨): 1. (英) Antibodies (日) (読) [継承]
2. (英) Antibody Affinity (日) (読) [継承]
3. (英) Circular Dichroism (日) (読) [継承]
4. (英) Thermodynamics (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Protein Journal ([Springer])
(pISSN: 1572-3887, eISSN: 1875-8355)

ISSN (任意): 1875-8355
ISSN: 1572-3887 (pISSN: 1572-3887, eISSN: 1875-8355)
Title: The protein journal
Title(ISO): Protein J
Supplier: Kluwer Online
Publisher: Springer
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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年月日 (必須): 西暦 2022年 4月 30日 (令和 4年 4月 30日) [継承]
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DOI (任意): 10.1007/s10930-022-10053-w    (→Scopusで検索) [継承]
PMID (任意): 35501455    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) PublicationType: Journal Article  (日)    [継承]
2.(英) PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Akihiro Nishiguchi, Akikazu Murakami, Takachika Azuma and Masayuki Oda : A Trade-off Between Thermostability and Binding Affinity of Anti-(4-hydroxy-3-nitrophenyl)Acetyl Antibodies During the Course of Affinity Maturation., The Protein Journal, Vol.41, No.2, 293-303, 2022.
欧文冊子 ● Akihiro Nishiguchi, Akikazu Murakami, Takachika Azuma and Masayuki Oda : A Trade-off Between Thermostability and Binding Affinity of Anti-(4-hydroxy-3-nitrophenyl)Acetyl Antibodies During the Course of Affinity Maturation., The Protein Journal, Vol.41, No.2, 293-303, 2022.

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