| ○種別 (必須): | □ | 学術論文 (審査論文)
| [継承] |
| ○言語 (必須): | □ | 英語
| [継承] |
| ○招待 (推奨): |
| ○審査 (推奨): | □ | Peer Review
| [継承] |
| ○カテゴリ (推奨): | □ | 研究
| [継承] |
| ○共著種別 (推奨): |
| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Tentaku Aya (日) 天宅あや (読) てんたくあや
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): | □ | ****
| [ユーザ] |
| [継承] |
| 2. | 栗栖 修作 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.生理系.細胞生物学])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 3. | (英) Sejima Kurumi (日) 瀬嶋来実 (読) せじまくるみ
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): | □ | ****
| [ユーザ] |
| [継承] |
| 4. | (英) Nagao Toshiki (日) 長尾俊紀 (読) ながおとしき
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): | □ | ****
| [ユーザ] |
| [継承] |
| 5. | 髙橋 章 ([徳島大学.大学院医歯薬学研究部.医学域.栄養科学部門.医科栄養学系.予防環境栄養学])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 6. | 米村 重信 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.生理系.細胞生物学])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| ○題名 (必須): | □ | (英) Proximal deposition of collagen IV by fibroblasts contributes to basement membrane formation by colon epithelial cells invitro. (日)
| [継承] |
| ○副題 (任意): |
| ○要約 (任意): | □ | (英) The basement membrane (BM) underlying epithelial tissue is a thin layer of extracellular matrix that governs tissue integrity and function. Epithelial BMs are generally assembled using BM components secreted from two origins: epithelium and stroma. Although denovo BM formation involves self-assembly processes of large proteins, it remains unclear how stroma-derived macromolecules are transported and assembled, specifically in the BM region. In this study, we established an invitro co-culture model of BM formation in which DLD-1 human colon epithelial cells were cultured on top of collagen I gel containing human embryonic OUMS-36T-2 fibroblasts as stromal cells. A distinct feature of our system is represented by OUMS-36T-2 cells which are almost exclusively responsible for synthesis of collagen IV, a major BM component. Exploiting this advantage, we found that collagen IV incorporation was significantly impaired in culture conditions where OUMS-36T-2 cells were not allowed to directly contact DLD-1 cells. Soluble collagen IV, once diluted in the culture medium, did not accumulate in the BM region efficiently. Live imaging of fluorescently tagged collagen IV revealed that OUMS-36T-2 cells deposited collagen IV aggregates directly onto the basal surface of DLD-1 cells. Collectively, these results indicate a novel mode of collagen IV deposition in which fibroblasts proximal to epithelial cells exclusively contribute to collagen IV assembly during BM formation. (日)
| [継承] |
| ○キーワード (推奨): | 1. | コラーゲン (collagen)
| [継承] |
| 2. | 細胞外マトリックス (extracellular matrix)
| [継承] |
| 3. | (英) co-culture (日) 共培養 (読)
| [継承] |
| 4. | (英) colon epithelium (日) 腸管上皮 (読)
| [継承] |
| 5. | (英) fibroblast (日) 線維芽細胞 (読)
| [継承] |
| ○発行所 (推奨): | □ | John Wiley & Sons, Inc.
| [継承] |
| ○誌名 (必須): | □ | The FEBS Journal ([Federation of European Biochemical Societies])
(pISSN: 1742-464X, eISSN: 1742-4658)
| ○ISSN (任意): | □ | 1742-4658
ISSN: 1742-464X
(pISSN: 1742-464X, eISSN: 1742-4658) Title: The FEBS journalTitle(ISO): FEBS JSupplier: Federation of European Biochemical SocietiesPublisher: Wiley Publishing (NLM Catalog)
(Wiley)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
| [継承] |
| ○巻 (必須): | □ | 289
| [継承] |
| ○号 (必須): | □ | 23
| [継承] |
| ○頁 (必須): | □ | 7466 7485
| [継承] |
| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2022年 12月 6日 (令和 4年 12月 6日)
| [継承] |
| ○URL (任意): |
| ○DOI (任意): | □ | 10.1111/febs.16559 (→Scopusで検索)
| [継承] |
| ○PMID (任意): | □ | 35730982 (→Scopusで検索)
| [継承] |
| ○CRID (任意): |
| ○WOS (任意): |
| ○Scopus (任意): |
| ○評価値 (任意): |
| ○被引用数 (任意): |
| ○指導教員 (推奨): |
| ○備考 (任意): | 1. | (英) PublicationType: Journal Article (日)
| [継承] |