| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○著者 (必須): | 1. | (英) Sugiyama Yasunori (日) (読)
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| 2. | 片山 将一 ([徳島大学.大学院医歯薬学研究部.薬学域.先端薬学教育研究プロジェクト]/[徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.生命薬学系.医薬品病態生化学])
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| 3. | (英) Kameshita Isamu (日) (読)
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| 4. | (英) Morisawa Keiko (日) (読)
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| 5. | (英) Higuchi Takuma (日) (読)
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| 6. | (英) Todaka Hiroshi (日) (読)
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| 7. | (英) Kinoshita Eiji (日) (読)
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| 8. | (英) Kinoshita-Kikuta Emiko (日) (読)
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| 9. | (英) Koike Tohru (日) (読)
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| 10. | (英) Taniguchi Taketoshi (日) (読)
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| 11. | (英) Sakamoto Shuji (日) (読)
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| ○題名 (必須): | □ | (英) Expression and phosphorylation state analysis of intracellular protein kinases using Multi-PK antibody and Phos-tag SDS-PAGE. (日)
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| ○要約 (任意): | □ | (英) (日) Protein kinase expression and activity play important roles in diverse cellular functions through regulation of phosphorylation signaling. The most commonly used tools for detecting the protein kinase are protein kinase-specific antibodies, and phosphorylation site-specific antibodies were used for detecting activated protein kinase. Using these antibodies, only one kinase was analyzed at a time, however, a method for analyzing the expression and activation of a panel of protein kinases in cells is not established. Therefore, we developed a combined method using Multi-PK antibody and Phos-tag SDS-PAGE for profiling the expression and phosphorylation state of intracellular protein kinases. Using the new method, changes in the expression and phosphorylation state of various protein kinases were detected in cells treated with anticancer agent which inhibit multiple tyrosine kinase activities. Therefore, the new method is a useful technique for analysis of intracellular protein kinases.•Multi-PK antibody recognizes a wide variety of protein kinases in various species.•Using Phos-tag SDS-PAGE, phosphorylated proteins are visualized as slower migration bands compared with corresponding non-phosphorylated proteins.•This combined method can be used for detecting changes in the expression and phosphorylation state of various intracellular protein kinases.
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| ○キーワード (推奨): |
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| ○誌名 (必須): | □ | MethodsX ([Elsevier])
(eISSN: 2215-0161)
| ○ISSN (任意): | □ | 2215-0161
ISSN: 2215-0161
(eISSN: 2215-0161) Title: MethodsXTitle(ISO): MethodsXPublisher: Elsevier B.V. (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 2
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| ○号 (必須): |
| ○頁 (必須): | □ | 469 474
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| ○年月日 (必須): | □ | 西暦 2015年 11月 19日 (平成 27年 11月 19日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/j.mex.2015.11.007 (→Scopusで検索)
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| ○PMID (任意): | □ | 26844212 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) PublicationType: Journal Article (日)
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