| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | 片山 将一 ([徳島大学.大学院医歯薬学研究部.薬学域.先端薬学教育研究プロジェクト]/[徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.生命薬学系.医薬品病態生化学])
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| 2. | (英) Senga Yukako (日) (読)
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| 3. | (英) Oi Ami (日) (読)
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| 4. | (英) Miki Yosuke (日) (読)
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| 5. | (英) Sugiyama Yasunori (日) (読)
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| 6. | (英) Sueyoshi Noriyuki (日) (読)
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| 7. | (英) Kameshita Isamu (日) (読)
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| ○題名 (必須): | □ | (英) Expression analyses of splice variants of zebrafish cyclin-dependent kinase-like 5 and its substrate, amphiphysin 1. (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) Mammalian cyclin-dependent kinase-like 5 (CDKL5) is a Ser/Thr protein kinase mainly expressed in the central nervous system and believed to be involved in neuronal functions. However, the functions of CDKL5 in fishes have not been investigated. Therefore, in this study, we cloned and characterized zebrafish CDKL5 (zCDKL5) and its substrate, amphiphysin 1 (zAmph1). Two alternative splice variants of zCDKL5, zCDKL5-Long (zCDKL5-L) and zCDKL5-Short (zCDKL5-S), and three splice variants of zAmph1, zAmph1a, zAmph1b and zAmph1c, were cloned from a zebrafish cDNA library. Using zAmph1a point mutants, we identified Ser-285 and Ser-293 as phosphorylation sites of zAmph1a by CDKL5. Transiently expressed zCDKL5-L and zCDKL5-S colocalized with zAmph1a in the cytoplasm of 293T cells. RT-PCR analysis revealed that zCDKL5-L was first observed 12hours post-fertilization (hpf) and increased thereafter, while zCDKL5-S appeared just after fertilization. zAmph1a was detected in all embryogenic stages and zAmph1b appeared from 12hpf, but the expression of zAmph1c was not observed in our experiments. In adult fish, zCDKL5-L was mainly expressed in the brain, but zCDKL5-S showed ubiquitous expression. zAmph1a was observed most abundantly in the eyes, whereas zAmph1b was predominantly expressed in the brain. zAmph1c was scarcely detected. These results suggest that phosphorylation of Amph1 by CDKL5 may be a common feature throughout animal species. (日)
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| ○キーワード (推奨): | 1. | (英) Alternative Splicing (日) (読)
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| 2. | (英) Amino Acid Sequence (日) (読)
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| 3. | (英) Animals (日) (読)
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| 4. | (英) Brain (日) (読)
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| 5. | (英) Cloning, Molecular (日) (読)
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| 6. | (英) Cytoplasm (日) (読)
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| 7. | (英) Embryo, Nonmammalian (日) (読)
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| 8. | (英) Gene Expression Regulation (日) (読)
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| 9. | (英) Molecular Sequence Data (日) (読)
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| 10. | (英) Nerve Tissue Proteins (日) (読)
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| 11. | (英) Phosphorylation (日) (読)
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| 12. | (英) Protein-Serine-Threonine Kinases (日) (読)
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| 13. | (英) Zebrafish (日) (読)
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| 14. | (英) Zebrafish Proteins (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Gene ([Elsevier Science B.V.])
(pISSN: 0378-1119, eISSN: 1879-0038)
| ○ISSN (任意): | □ | 1879-0038
ISSN: 0378-1119
(pISSN: 0378-1119, eISSN: 1879-0038) Title: GeneTitle(ISO): GenePublisher: Elsevier B.V. (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 583
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| ○号 (必須): | □ | 1
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| ○頁 (必須): | □ | 15 23
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2016年 2月 27日 (平成 28年 2月 27日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/j.gene.2016.02.036 (→Scopusで検索)
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| ○PMID (任意): | □ | 26927518 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) PublicationType: Journal Article (日)
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| 2. | (英) PublicationType: Research Support, Non-U.S. Gov't (日)
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