| ○種別 (必須): | □ | 学術論文 (審査論文)
| [継承] |
| ○言語 (必須): | □ | 英語
| [継承] |
| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): |
| ○共著種別 (推奨): |
| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | 片山 将一 ([徳島大学.大学院医歯薬学研究部.薬学域.先端薬学教育研究プロジェクト]/[徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.生命薬学系.医薬品病態生化学])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 2. | (英) Morii Atsushi (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 3. | (英) Makanga Juliet O (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 4. | (英) Suzuki Takayoshi (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 5. | (英) Miyata Naoki (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 6. | (英) Inazu Tetsuya (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| ○題名 (必須): | □ | (英) (日) HDAC8 regulates neural differentiation through embryoid body formation in P19 cells.
| [継承] |
| ○副題 (任意): |
| ○要約 (任意): | □ | (英) (日) Histone acetylation and deacetylation correlate with diverse biological phenomena through gene transcription. Histone deacetylases (HDACs) regulate deacetylation of histones and other proteins. However, as a member of the HDAC family, HDAC8 function during neurodevelopment is currently unknown. Therefore, we investigated HDAC8 function during neurodevelopment by examining embryoid body (EB) formation in P19 cells. HDAC8-selective inhibitor (NCC-149) (HDAC8i)-treated cells showed smaller EBs than non-treated cells, as well as reduced expression levels of the neuronal marker, NeuN. Additionally, HDAC8i treatment led to inhibition of cellular proliferation by G2/M phase accumulation and downregulated cyclin A2 and cyclin B1 gene expression. Furthermore, two independent HDAC8 knockout cell lines were established by CRISPR-Cas9, which resulted in smaller EBs, similar to HDAC8i-treated cells. These results suggest that HDAC8 regulates neural differentiation by exerting control of EB formation.
| [継承] |
| ○キーワード (推奨): | 1. | (英) Animals (日) (読)
| [継承] |
| 2. | (英) Base Sequence (日) (読)
| [継承] |
| 3. | (英) Cell Cycle Checkpoints (日) (読)
| [継承] |
| 4. | (英) Cell Differentiation (日) (読)
| [継承] |
| 5. | (英) Cell Line, Tumor (日) (読)
| [継承] |
| 6. | (英) Cell Proliferation (日) (読)
| [継承] |
| 7. | (英) Cyclin A1 (日) (読)
| [継承] |
| 8. | (英) Cyclin B2 (日) (読)
| [継承] |
| 9. | (英) Down-Regulation (日) (読)
| [継承] |
| 10. | (英) Embryoid Bodies (日) (読)
| [継承] |
| 11. | (英) G2 Phase (日) (読)
| [継承] |
| 12. | (英) Gene Expression Regulation, Neoplastic (日) (読)
| [継承] |
| 13. | (英) Gene Knockout Techniques (日) (読)
| [継承] |
| 14. | (英) Histone Deacetylase Inhibitors (日) (読)
| [継承] |
| 15. | (英) Histone Deacetylases (日) (読)
| [継承] |
| 16. | (英) Humans (日) (読)
| [継承] |
| 17. | (英) Mice (日) (読)
| [継承] |
| 18. | (英) Mitosis (日) (読)
| [継承] |
| 19. | (英) Neurons (日) (読)
| [継承] |
| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Biochemical and Biophysical Research Communications ([Elsevier])
(pISSN: 0006-291X, eISSN: 1090-2104)
| ○ISSN (任意): | □ | 1090-2104
ISSN: 0006-291X
(pISSN: 0006-291X, eISSN: 1090-2104) Title: Biochemical and biophysical research communicationsTitle(ISO): Biochem Biophys Res CommunPublisher: Elsevier B.V. (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
| [継承] |
| ○巻 (必須): | □ | 498
| [継承] |
| ○号 (必須): | □ | 1
| [継承] |
| ○頁 (必須): | □ | 45 51
| [継承] |
| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2018年 2月 28日 (平成 30年 2月 28日)
| [継承] |
| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/j.bbrc.2018.02.195 (→Scopusで検索)
| [継承] |
| ○PMID (任意): | □ | 29499194 (→Scopusで検索)
| [継承] |
| ○CRID (任意): |
| ○WOS (任意): |
| ○Scopus (任意): |
| ○評価値 (任意): |
| ○被引用数 (任意): |
| ○指導教員 (推奨): |
| ○備考 (任意): | 1. | (英) PublicationType: Journal Article (日)
| [継承] |
| 2. | (英) PublicationType: Research Support, Non-U.S. Gov't (日)
| [継承] |