| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○審査 (推奨): |
| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
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| ○著者 (必須): | 1. | 片山 将一 ([徳島大学.大学院医歯薬学研究部.薬学域.先端薬学教育研究プロジェクト]/[徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.生命薬学系.医薬品病態生化学])
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| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
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| 2. | (英) Inazu Tetsuya (日) (読)
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| ○題名 (必須): | □ | (英) Straightforward and rapid method for detection of cyclin-dependent kinase-like 5 activity. (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) Cyclin-dependent kinase-like 5 (CDKL5) is a serine/threonine protein kinase, with its gene mutation leading to a neurodevelopmental disorder. Pathogenic point mutations are mostly observed within the catalytic domain of CDKL5, therefore loss of catalytic activity may be related to disease onset. However, this hypothesis has rarely been demonstrated. Here, we report an efficient method for detecting CDKL5 activity. Appropriately, CDKL5 underwent autophosphorylation following expression in Escherichia coli, with autophosphorylated CDKL5 detected as a band shift by phos-tag SDS-PAGE, without enzyme purification. Thus, this protocol is useful for examining the relationship between disease-causing mutations and their activity. (日)
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| ○キーワード (推奨): | 1. | (英) Catalytic Domain (日) (読)
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| 2. | (英) Cloning, Molecular (日) (読)
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| 3. | (英) Enzyme Assays (日) (読)
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| 4. | (英) Escherichia coli (日) (読)
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| 5. | (英) Mutation, Missense (日) (読)
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| 6. | (英) Phosphorylation (日) (読)
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| 7. | (英) Protein-Serine-Threonine Kinases (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Analytical Biochemistry: Methods in the Biological Sciences ([Elsevier Science])
(pISSN: 0003-2697, eISSN: 1096-0309)
| ○ISSN (任意): | □ | 1096-0309
ISSN: 0003-2697
(pISSN: 0003-2697, eISSN: 1096-0309) Title: Analytical biochemistryTitle(ISO): Anal BiochemPublisher: Academic Press Inc. (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 566
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| ○号 (必須): |
| ○頁 (必須): | □ | 58 61
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2018年 11月 14日 (平成 30年 11月 14日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/j.ab.2018.11.013 (→Scopusで検索)
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| ○PMID (任意): | □ | 30447183 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) PublicationType: Journal Article (日)
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| 2. | (英) PublicationType: Research Support, Non-U.S. Gov't (日)
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