『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=377949)

EID=377949EID:377949, Map:0, LastModified:2021年8月10日(火) 12:57:44, Operator:[大家 隆弘], Avail:TRUE, Censor:承認済, Owner:[田原 強], Read:継承, Write:継承, Delete:継承.
○種別 (必須): □学術論文 (審査論文) [継承]
○言語 (必須): □英語 [継承]
○招待 (推奨):
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○カテゴリ (推奨):
○共著種別 (推奨):
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○組織 (推奨):
○著者 (必須): 1.田原 強 ([徳島大学.先端研究推進センター.バイオイメージング研究部門])
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○学籍番号 (推奨):
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2. (英) Sun Jiying (日) (読)
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3. (英) Nakanishi Katsuyuki (日) (読)
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4. (英) Yamamoto Masafumi (日) (読)
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5. (英) Mori Hajime (日) (読)
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6. (英) Saito Takeshi (日) (読)
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7. (英) Fujita Hiroyoshi (日) (読)
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8. (英) Igarashi Kazuhiko (日) (読)
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9. (英) Taketani Shigeru (日) (読)
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○題名 (必須): □(英) Heme positively regulates the expression of beta-globin at the locus control region via the transcriptional factor Bach1 in erythroid cells.  (日)    [継承]
○副題 (任意):
○要約 (任意): □(英) The transcription factor Bach1 heterodimerizes with small Maf proteins to repress Maf recognition element (MARE)-dependent gene expression. The repressor activity of Bach1 is inhibited by the direct binding of heme. To investigate the involvement of Bach1 in the heme-dependent regulation of the expression of the beta-globin gene, mouse erythroleukemia (MEL) cells were cultured with succinylacetone (SA), a specific inhibitor of heme biosynthesis, and the level of beta-globin mRNA was examined. A marked decrease of beta-globin mRNA in SA-treated cells was observed, and this decrease was reversed by the addition of hemin. An iron chelator, desferrioxamine, also lowered the level of beta-globin mRNA. The heme-dependent expression of beta-globin is a transcriptional event since the expression of the human beta-globin gene promoter-reporter gene containing the microlocus control region (microLCR) was inhibited when human erythroleukemia K562 cells and MEL cells were cultured with SA. Hemin treatment restored the decrease in promoter activity caused by SA. The control of the microLCR-beta-globin promoter reporter gene by heme was dependent on DNase I-hypersensitive site 2 (HS2), which contains MARE. The MARE binding activity of Bach1 in K562 and MEL cells increased upon SA treatment, and the increase was diminished by the treatment with hemin. Transient expression of Bach1 suppressed the microLCR activity, and this repressor activity was cancelled by treatment with hemin. The expression of a mutated Bach1 lacking heme-binding sites led to a loss in the heme responsiveness of the microLCR. Furthermore, chromatin immunoprecipitation experiments revealed that Bach1 bound to the MARE of HS2 increased by the treatment of MEL cells with SA, and this was cancelled by hemin. We propose that heme positively regulates the beta-globin gene expression by blocking the interaction of Bach1 with the MARE in the LCR.  (日)    [継承]
○キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Basic-Leucine Zipper Transcription Factors (日) (読) [継承]
3. (英) Binding Sites (日) (読) [継承]
4. (英) Blotting, Northern (日) (読) [継承]
5. (英) Cell Line (日) (読) [継承]
6. (英) Chelating Agents (日) (読) [継承]
7. (英) Chromatin (日) (読) [継承]
8. (英) Deferoxamine (日) (読) [継承]
9. (英) Dimerization (日) (読) [継承]
10. (英) Erythroid Cells (日) (読) [継承]
11. (英) Fanconi Anemia Complementation Group Proteins (日) (読) [継承]
12. (英) Gene Deletion (日) (読) [継承]
13. (英) Gene Expression Regulation (日) (読) [継承]
14. (英) Genes, Reporter (日) (読) [継承]
15. (英) Globins (日) (読) [継承]
16. (英) Heme (日) (読) [継承]
17. (英) Heptanoates (日) (読) [継承]
18. (英) Humans (日) (読) [継承]
19. (英) Iron (日) (読) [継承]
20. (英) K562 Cells (日) (読) [継承]
21. (英) Leukemia, Erythroblastic, Acute (日) (読) [継承]
22. (英) Mice (日) (読) [継承]
23. (英) Mice, Inbred BALB C (日) (読) [継承]
24. (英) Plasmids (日) (読) [継承]
25. (英) Precipitin Tests (日) (読) [継承]
26. (英) Promoter Regions, Genetic (日) (読) [継承]
27. (英) Protein Binding (日) (読) [継承]
28. (英) RNA (日) (読) [継承]
29. (英) RNA, Messenger (日) (読) [継承]
30. (英) Transcription Factors (日) (読) [継承]
○発行所 (推奨):
○誌名 (必須): □The Journal of Biological Chemistry ([The American Society for Biochemistry and Molecular Biology])
(pISSN: 0021-9258, eISSN: 1083-351X)

○ISSN (任意): □0021-9258
ISSN: 0021-9258 (pISSN: 0021-9258, eISSN: 1083-351X)
Title: The Journal of biological chemistry
Title(ISO): J Biol Chem
Publisher: American Society for Biochemistry and Molecular Biology
 (NLM Catalog)   (Scopus)   (CrossRef)  (Scopus information is found. [need login])
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○巻 (必須): □279 [継承]
○号 (必須): □7 [継承]
○頁 (必須): □5480 5487 [継承]
○都市 (任意):
○年月日 (必須): □西暦 2003年 12月 1日 (平成 15年 12月 1日) [継承]
○URL (任意):
○DOI (任意): □10.1074/jbc.M302733200    (→Scopusで検索) [継承]
○PMID (任意): □14660636    (→Scopusで検索) [継承]
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○備考 (任意): 1.(英) PublicationType: Journal Article  (日)    [継承]
2.(英) PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Tsuyoshi Tahara, Jiying Sun, Katsuyuki Nakanishi, Masafumi Yamamoto, Hajime Mori, Takeshi Saito, Hiroyoshi Fujita, Kazuhiko Igarashi and Shigeru Taketani : Heme positively regulates the expression of beta-globin at the locus control region via the transcriptional factor Bach1 in erythroid cells., The Journal of Biological Chemistry, Vol.279, No.7, 5480-5487, 2003.
欧文冊子 ● Tsuyoshi Tahara, Jiying Sun, Katsuyuki Nakanishi, Masafumi Yamamoto, Hajime Mori, Takeshi Saito, Hiroyoshi Fujita, Kazuhiko Igarashi and Shigeru Taketani : Heme positively regulates the expression of beta-globin at the locus control region via the transcriptional factor Bach1 in erythroid cells., The Journal of Biological Chemistry, Vol.279, No.7, 5480-5487, 2003.

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