『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
ID: Pass:

登録内容 (EID=374711)

EID=374711EID:374711, Map:0, LastModified:2021年8月24日(火) 17:38:33, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[福井 清], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 教育 [継承]
共著種別 (推奨): 単独著作 (徳島大学内の単一の研究グループ(研究室等)内の研究 (単著も含む)) [継承]
学究種別 (推奨): 博士課程学生による研究報告 [継承]
組織 (推奨): 1.徳島大学.先端酵素学研究所 (2016年4月1日〜) [継承]
著者 (必須): 1. (英) Sogabe Hirofumi (日) 曽我部 浩史 (読) そがべ ひろふみ
役割 (任意):
貢献度 (任意):
学籍番号 (推奨): **** [ユーザ]
[継承]
2.宍戸 裕二 ([徳島大学.研究支援・産官学連携センター])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Miyazaki Hayato (日) 宮崎 隼人 (読) みやざき はやと
役割 (任意):
貢献度 (任意):
学籍番号 (推奨): **** [ユーザ]
[継承]
4. (英) Kim SooHyeon (日) Kim Soo Hyeon (読) きむ すひょん
役割 (任意):
貢献度 (任意):
学籍番号 (推奨): **** [ユーザ]
[継承]
5. (英) Rachadech Wanitcha (日) Wanitcha Rachadech (読) わにちゃ らしゃで
役割 (任意):
貢献度 (任意):
学籍番号 (推奨): **** [ユーザ]
[継承]
6.福井 清 ([徳島大学])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Dynamics of D-amino acid oxidase in kidney epithelial cells under amino acid starvation  (日)    [継承]
副題 (任意):
要約 (任意): (英) D-amino acid oxidase (DAO) is a flavoenzyme catalyzing the oxidation of D-amino acid (AA)s. In the kidney, its expression is detected in proximal tubules, and DAO is considered to play a role in the conversion of D-form AAs to α-keto acids. LLC-PK1 cells, a pig renal proximal tubule cell line, were used to elucidate the regulation of DAO protein synthesis and degradation. In this study, we showed that trypsinization of LLC-PK1 cells in culture system rapidly reduced the intracellular DAO protein level to approximately 33.9% of that before treatment, even within 30 min. Furthermore, we observed that the DAO protein level was decreased when LLC-PK1 cells were subjected to AA starvation. To determine the degradation pathway, we treated the cells with chloroquine and MG132. DAO degradation was found to be inhibited by chloroquine, but not by MG132 treatment. We next examined whether or not DAO was degraded by autophagy. We found that AA starvation led to an increased accumulation of LC3-II, suggesting that DAO protein is degraded by autophagy due to AA starvation conditions. Furthermore, treatment with cycloheximide inhibited DAO protein degradation. Taken together, DAO protein is degraded by autophagy under starvation. The present study revealed the potential dynamics of DAO correlated with renal pathophysiology.  (日)    [継承]
キーワード (推奨):
発行所 (推奨): 日本生化学会 [継承]
誌名 (必須): The Journal of Biochemistry ([日本生化学会])
(pISSN: 0021-924X, eISSN: 1756-2651)

ISSN (任意): 1756-2651
ISSN: 0021-924X (pISSN: 0021-924X, eISSN: 1756-2651)
Title: Journal of biochemistry
Title(ISO): J Biochem
Supplier: Oxford University Press
Publisher: Oxford University Press
 (NLM Catalog  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須):
(必須):
(必須):
都市 (任意):
年月日 (必須): 西暦 2022年 0月 初日 (令和 4年 0月 初日) [継承]
URL (任意):
DOI (任意): 10.1093/jb/mvab029    (→Scopusで検索) [継承]
PMID (任意): 33725110    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意):
Scopus (任意):
評価値 (任意):
被引用数 (任意):
指導教員 (推奨): 1.福井 清 ([徳島大学]) [継承]
備考 (任意): 1.(英) Article.ELocationID: mvab029  (日)    [継承]
2.(英) Article.ELocationID: 10.1093/jb/mvab029  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Hirofumi Sogabe, Yuji Shishido, Hayato Miyazaki, SooHyeon Kim, Wanitcha Rachadech and Kiyoshi Fukui : Dynamics of D-amino acid oxidase in kidney epithelial cells under amino acid starvation, The Journal of Biochemistry, (巻), (号), (頁), 2022.
欧文冊子 ● Hirofumi Sogabe, Yuji Shishido, Hayato Miyazaki, SooHyeon Kim, Wanitcha Rachadech and Kiyoshi Fukui : Dynamics of D-amino acid oxidase in kidney epithelial cells under amino acid starvation, The Journal of Biochemistry, (巻), (号), (頁), 2022.

関連情報

Number of session users = 0, LA = 0.38, Max(EID) = 382712, Max(EOID) = 1022125.