『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=373149EID:373149, Map:0, LastModified:2021年1月25日(月) 20:24:04, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[鬼塚 正義], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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カテゴリ (推奨): 教育 [継承]
共著種別 (推奨):
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組織 (推奨):
著者 (必須): 1. (英) Yagi Hirokazu (日) (読)
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2. (英) Yanaka Saeko (日) (読)
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3. (英) Yogo Rina (日) (読)
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4. (英) Ikeda Akari (日) (読)
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5.鬼塚 正義 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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6. (英) Yamazaki Toshio (日) (読)
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7. (英) Kato Ttsuya (日) (読)
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8. (英) Park Y. Enoch (日) (読)
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9. (英) Yokoyama Jun (日) (読)
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10. (英) Kato Koichi (日) (読)
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題名 (必須): (英) Silkworm pupae function as efficient producers of recombinant glycoproteins with stable-isotope labeling.  (日)    [継承]
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要約 (任意): (英) Baculovirus-infected silkworms are promising bioreactors for producing recombinant glycoproteins, including antibodies. Previously, we developed a method for isotope labeling of glycoproteins for nuclear magnetic resonance (NMR) studies using silkworm larvae reared on an artificial diet containing N-labeled yeast crude protein extract. Here, we further develop this method by introducing a technique for the expression of isotope-labeled glycoproteins by silkworm pupae, which has several potential advantages relative to larvae-based techniques in terms of production yield, ease of handling, and storage. Here, we fed fifth instar larvae an artificial diet with an optimized composition containing [methyl-C]methionine, leading to pupation. Nine-day-old pupae were then injected with recombinant nucleopolyhedrovirus (BmNPV) bacmid for expression of recombinant human immunoglobulin G (IgG). From the whole-body homogenates of pupae, 0.35 mg/pupa of IgG was harvested, which is a yield that is five times higher than can be obtained from larvae. Recombinant IgG, thus prepared, exhibited mainly three kinds of pauci-mannose-type oligosaccharides and had a C-enrichment ratio of approximately 80%. This enabled selective observation of NMR signals originating from the methionyl methyl group of IgG, confirming its conformational integrity. These data demonstrate the utility of silkworm pupae as factories for producing recombinant glycoproteins with amino-acid-selective isotope labeling.  (日)    [継承]
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誌名 (必須): Biomolecules (Molecular Diversity Preservation International)
(eISSN: 2218-273X)

ISSN (任意): 2218-273X
ISSN: 2218-273X (eISSN: 2218-273X)
Title: Biomolecules
Title(ISO): Biomolecules
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 10 [継承]
(必須): 11 [継承]
(必須): 1482 1482 [継承]
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年月日 (必須): 西暦 2020年 10月 26日 (令和 2年 10月 26日) [継承]
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DOI (任意): 10.3390/biom10111482    (→Scopusで検索) [継承]
PMID (任意): 33114581    (→Scopusで検索) [継承]
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Scopus (任意): 2-s2.0-85094185161 [継承]
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備考 (任意): 1.(英) Article.ELocationID: E1482  (日)    [継承]
2.(英) Article.ELocationID: 10.3390/biom10111482  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
4.(英) KeywordList.Keyword: artificial diet  (日)    [継承]
5.(英) KeywordList.Keyword: immunoglobulin G  (日)    [継承]
6.(英) KeywordList.Keyword: isotope labeling  (日)    [継承]
7.(英) KeywordList.Keyword: recombinant glycoprotein  (日)    [継承]
8.(英) KeywordList.Keyword: silkworm pupa  (日)    [継承]

標準的な表示

和文冊子 ● Hirokazu Yagi, Saeko Yanaka, Rina Yogo, Akari Ikeda, Masayoshi Onitsuka, Toshio Yamazaki, Ttsuya Kato, Enoch Y. Park, Jun Yokoyama and Koichi Kato : Silkworm pupae function as efficient producers of recombinant glycoproteins with stable-isotope labeling., Biomolecules, Vol.10, No.11, 1482, 2020.
欧文冊子 ● Hirokazu Yagi, Saeko Yanaka, Rina Yogo, Akari Ikeda, Masayoshi Onitsuka, Toshio Yamazaki, Ttsuya Kato, Enoch Y. Park, Jun Yokoyama and Koichi Kato : Silkworm pupae function as efficient producers of recombinant glycoproteins with stable-isotope labeling., Biomolecules, Vol.10, No.11, 1482, 2020.

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