『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
ID: Pass:

登録内容 (EID=373147)

EID=373147EID:373147, Map:0, LastModified:2021年1月25日(月) 20:21:14, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[鬼塚 正義], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Ryutaro Asano (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Katsuhiro Hosokawa (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Shintaro Taki (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Shota Konno (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
5. (英) Ippei Shimomura (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
6. (英) Hiromi Ogata (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
7. (英) Mai Okada (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
8. (英) Kyoko Arai (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
9.鬼塚 正義 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
10.大政 健史
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
11. (英) Takeshi Nakanishi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
12. (英) Mitsuo Umetsu (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
13. (英) Izumi Kumagai (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Build-up functionalization of anti-EGFR × anti-CD3 bispecific diabodies by integrating high-affinity mutants and functional molecular formats.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Designing non-natural antibody formats is a practical method for developing highly functional next-generation antibody drugs, particularly for improving the therapeutic efficacy of cancer treatments. One approach is constructing bispecific antibodies (bsAbs). We previously reported a functional humanized bispecific diabody (bsDb) that targeted epidermal growth factor receptor and CD3 (hEx3-Db). We enhanced its cytotoxicity by constructing an Fc fusion protein and rearranging order of the V domain. In this study, we created an additional functional bsAb, by integrating the molecular formats of bsAb and high-affinity mutants previously isolated by phage display in the form of Fv. Introducing the high-affinity mutations into bsDbs successfully increased their affinities and enhanced their cytotoxicity in vitro and in vivo. However, there were some limitations to affinity maturation of bsDb by integrating high-affinity Fv mutants, particularly in Fc-fused bsDb with intrinsic high affinity, because of their bivalency. The tetramers fractionated from the bsDb mutant exhibited the highest in vitro growth inhibition among the small bsAbs and was comparable to the in vivo anti-tumor effects of Fc-fused bsDbs. This molecule shows cost-efficient bacterial production and high therapeutic potential.  (日)    [継承]
キーワード (推奨): 1. (英) Antibodies, Bispecific (日) (読) [継承]
2. (英) Antineoplastic Agents, Immunological (日) (読) [継承]
3. (英) CD3 Complex (日) (読) [継承]
4. (英) Drug Design (日) (読) [継承]
5. (英) ErbB Receptors (日) (読) [継承]
6. (英) Mutation (日) (読) [継承]
7. (英) Protein Binding (日) (読) [継承]
8. (英) Protein Engineering (日) (読) [継承]
9. (英) Recombinant Fusion Proteins (日) (読) [継承]
10. (英) Structure-Activity Relationship (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Scientific Reports ([Nature Publishing Group])
(eISSN: 2045-2322)

ISSN (任意): 2045-2322
ISSN: 2045-2322 (eISSN: 2045-2322)
Title: Scientific reports
Title(ISO): Sci Rep
Publisher: Springer Science+Business Media
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 10 [継承]
(必須): [継承]
(必須): 4913 4913 [継承]
都市 (任意):
年月日 (必須): 西暦 2020年 4月 初日 (令和 2年 4月 初日) [継承]
URL (任意):
DOI (任意): 10.1038/s41598-020-61840-3    (→Scopusで検索) [継承]
PMID (任意): 32188928    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意):
Scopus (任意): 2-s2.0-85082085940 [継承]
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意): 1.(英) Article.ELocationID: 10.1038/s41598-020-61840-3  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Asano Ryutaro, Hosokawa Katsuhiro, Taki Shintaro, Konno Shota, Shimomura Ippei, Ogata Hiromi, Okada Mai, Arai Kyoko, Masayoshi Onitsuka, Takeshi Omasa, Nakanishi Takeshi, Umetsu Mitsuo and Kumagai Izumi : Build-up functionalization of anti-EGFR × anti-CD3 bispecific diabodies by integrating high-affinity mutants and functional molecular formats., Scientific Reports, Vol.10, 4913, 2020.
欧文冊子 ● Asano Ryutaro, Hosokawa Katsuhiro, Taki Shintaro, Konno Shota, Shimomura Ippei, Ogata Hiromi, Okada Mai, Arai Kyoko, Masayoshi Onitsuka, Takeshi Omasa, Nakanishi Takeshi, Umetsu Mitsuo and Kumagai Izumi : Build-up functionalization of anti-EGFR × anti-CD3 bispecific diabodies by integrating high-affinity mutants and functional molecular formats., Scientific Reports, Vol.10, 4913, 2020.

関連情報

Number of session users = 0, LA = 0.89, Max(EID) = 374116, Max(EOID) = 1001822.