| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○審査 (推奨): |
| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Takahashi Sayo (日) 高橋 早代 (読) たかはし さよ
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| ○学籍番号 (推奨): | □ | ****
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| 2. | 岡本 耕一 ([徳島大学.病院.診療科.内科.消化器内科])
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| 3. | 棚橋 俊仁
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| 4. | (英) Fujimoto Shota (日) 藤本 将太 (読) ふじもと しょうた
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| ○学籍番号 (推奨): | □ | ****
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| 5. | 中川 忠彦
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| ○学籍番号 (推奨): | □ | ****
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| 6. | 板東 正浩 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.内科系.消化器内科学])
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| ○学籍番号 (推奨): | □ | ****
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| 7. | (英) ma beibei (日) 馬 貝貝 (読) ま べいべい
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| ○学籍番号 (推奨): | □ | ****
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| 8. | 川口 智之 ([徳島大学.病院.診療科.内科.消化器内科])
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| ○学籍番号 (推奨): | □ | ****
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| 9. | 藤野 泰輝
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| ○学籍番号 (推奨): | □ | ****
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| 10. | 三井 康裕 ([徳島大学.大学院医歯薬学研究部.医学域.先端医学教育研究プロジェクト]/[徳島大学.大学院医歯薬学研究部.医学域.医科学部門.内科系.消化器内科学])
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| ○学籍番号 (推奨): | □ | ****
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| 11. | 北村 晋志
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| 12. | 宮本 弘志 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.内科系.消化器内科学]/[徳島大学.病院.診療科.内科.消化器内科])
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| 13. | 佐藤 康史 ([徳島大学.大学院医歯薬学研究部.医学域.連携研究部門(医学域).寄附講座系(医学域).地域消化器・総合内科学])
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| 14. | 六車 直樹
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| 15. | 坂東 良美 ([徳島大学.病院.診療科.病理診断科])
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| 16. | (英) Sato Toshiro (日) (読)
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| 17. | (英) Fujimori Takahiro (日) (読)
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| 18. | 高山 哲治 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.内科系.消化器内科学])
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| ○題名 (必須): | □ | (英) S100P expression via DNA hypomethylation promotes cell growth in the sessile serrated adenoma/polyp-cancer sequence. (日)
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| ○要約 (任意): | □ | (英) Sessile serrated adenomas/polyps (SSA/Ps) are a putative precursor lesion of colon cancer. Although the relevance of DNA hypermethylation in the SSA/P-cancer sequence is well documented, the role of DNA hypomethylation is unknown. We investigated the biological relevance of DNA hypomethylation in the SSA/P-cancer sequence by using 3-dimensional organoids of SSA/P. We first analyzed hypomethylated genes using datasets from our previous DNA methylation array analysis on 7 SSA/P and 2 cancer in SSA/P specimens. Expression levels of hypomethylated genes in SSA/P specimens were determined by RT-PCR and immunohistochemistry. We established 3-dimensional SSA/P organoids and performed knockdown experiments using a lentiviral shRNA vector. DNA hypomethylation at CpG sites of the gene was quantitated by MassARRAY analysis. The mean number of hypomethylated genes in SSA/P and cancer in SSA/P was 41.6 ± 27.5 and 214 ± 19.8, respectively, showing a stepwise increment in hypomethylation during the SSA/P-cancer sequence. S100P, S100α2, PKP3, and MUC2 were most commonly hypomethylated in SSA/P specimens. The mRNA and protein expression levels of S100P, S100α2, and MUC2 were significantly elevated in SSA/P compared with normal colon tissues, as revealed by RT-PCR and immunohistochemistry, respectively. Among these, mRNA and protein levels were highest for S100P. Knockdown of the S100P gene using a lentiviral shRNA vector in 3-dimensional SSA/P organoids inhibited cell growth by >50% (p < 0.01). The mean diameter of SSA/P organoids with S100P gene knockdown was significantly smaller compared with control organoids. MassARRAY analysis of DNA hypomethylation in the S100P gene revealed significant hypomethylation at specific CpG sites in intron 1, exon 1, and the 5'-flanking promoter region. These results suggest that DNA hypomethylation, including S100P hypomethylation, is supposedly associated with the SSA/P-cancer sequence. S100P overexpression via DNA hypomethylation plays an important role in promoting cell growth in the SSA/P-cancer sequence. (日)
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| ○キーワード (推奨): |
| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Digestion ([Karger])
(pISSN: 0012-2823, eISSN: 1421-9867)
| ○ISSN (任意): | □ | 1421-9867
ISSN: 0012-2823
(pISSN: 0012-2823, eISSN: 1421-9867) Title: DigestionTitle(ISO): DigestionPublisher: S. Karger AG (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 102
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| ○号 (必須): | □ | 5
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| ○頁 (必須): | □ | 789 802
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2021年 1月 4日 (令和 3年 1月 4日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1159/000512575 (→Scopusで検索)
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| ○PMID (任意): | □ | 33395688 (→Scopusで検索)
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| ○CRID (任意): |
| ○WOS (任意): |
| ○Scopus (任意): | □ | 2-s2.0-85099095900
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| ○評価値 (任意): |
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| ○指導教員 (推奨): |
| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1159/000512575 (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) KeywordList.Keyword: 3-dimensional organoid (日)
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| 4. | (英) KeywordList.Keyword: DNA hypomethylation (日)
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| 5. | (英) KeywordList.Keyword: Genome-wide DNA methylation array (日)
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| 6. | (英) KeywordList.Keyword: S100P (日)
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| 7. | (英) KeywordList.Keyword: Sessile serrated adenoma/polyp (日)
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