『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨): 国内共著 (徳島大学内研究者と国内(学外)研究者との共同研究 (国外研究者を含まない)) [継承]
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.河北 直也 ([徳島大学.病院.中央診療施設等.卒後臨床研修センター])
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[継承]
2.鳥羽 博明 ([徳島大学.大学院医歯薬学研究部.医学域.先端医学教育研究プロジェクト]/[徳島大学.大学院医歯薬学研究部.医学域.医科学部門.外科系.胸部・内分泌・腫瘍外科学])
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学籍番号 (推奨):
[継承]
3.三好 圭子 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔生命科学])
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4. (英) Sakamoto Shinichi (日) 坂本 晋一 (読) さかもと しんいち
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学籍番号 (推奨): **** [ユーザ]
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5.松本 大資
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学籍番号 (推奨): **** [ユーザ]
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6.髙嶋 美佳 ([徳島大学.病院.診療科.外科.食道・乳腺甲状腺外科])
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学籍番号 (推奨): **** [ユーザ]
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7.青山 万理子 ([徳島大学.大学院医歯薬学研究部.医学域.連携研究部門(医学域).寄附講座系(医学域).実践地域診療・医科学])
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8.井上 聖也 ([徳島大学.病院.先端医科医療開発研究プロジェクト]/[徳島大学.病院.診療科.外科.食道・乳腺甲状腺外科])
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学籍番号 (推奨): **** [ユーザ]
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9.森本 雅美
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10.西野 豪志
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11.滝沢 宏光 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.外科系.胸部・内分泌・腫瘍外科学]/[徳島大学.病院.診療科.外科.呼吸器外科])
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12.丹黒 章 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.外科系.胸部・内分泌・腫瘍外科学])
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題名 (必須): (英) Bronchioalveolar stem cells derived from mouse-induced pluripotent stem cells promote airway epithelium regeneration  (日)    [継承]
副題 (任意):
要約 (任意): (英) Bronchioalveolar stem cells (BASCs) located at the bronchioalveolar-duct junction (BADJ) are stem cells residing in alveoli and terminal bronchioles that can self-renew and differentiate into alveolar type (AT)-1 cells, AT-2 cells, club cells, and ciliated cells. Following terminal-bronchiole injury, BASCs increase in number and promote repair. However, whether BASCs can be differentiated from mouse-induced pluripotent stem cells (iPSCs) remains unreported, and the therapeutic potential of such cells is unclear. We therefore sought to differentiate BASCs from iPSCs and examine their potential for use in the treatment of epithelial injury in terminal bronchioles. BASCs were induced using a modified protocol for differentiating mouse iPSCs into AT-2 cells. Differentiated iPSCs were intratracheally transplanted into naphthalene-treated mice. The engraftment of BASCs into the BADJ and their subsequent ability to promote repair of injury to the airway epithelium were evaluated. Flow cytometric analysis revealed that BASCs represented ~ 7% of the cells obtained. Additionally, ultrastructural analysis of these iPSC-derived BASCs via transmission electron microscopy showed that the cells containing secretory granules harboured microvilli, as well as small and immature lamellar body-like structures. When the differentiated iPSCs were intratracheally transplanted in naphthalene-induced airway epithelium injury, transplanted BASCs were found to be engrafted in the BADJ epithelium and alveolar spaces for 14 days after transplantation and to maintain the BASC phenotype. Notably, repair of the terminal-bronchiole epithelium was markedly promoted after transplantation of the differentiated iPSCs. Mouse iPSCs could be differentiated in vitro into cells that display a similar phenotype to BASCs. Given that the differentiated iPSCs promoted epithelial repair in the mouse model of naphthalene-induced airway epithelium injury, this method may serve as a basis for the development of treatments for terminal-bronchiole/alveolar-region disorders.  (日)    [継承]
キーワード (推奨):
発行所 (推奨):
誌名 (必須): Stem Cell Research & Therapy ([BioMed Central Ltd.])
(eISSN: 1757-6512)

ISSN (任意): 1757-6512
ISSN: 1757-6512 (eISSN: 1757-6512)
Title: Stem cell research & therapy
Title(ISO): Stem Cell Res Ther
Publisher: BioMed Central
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 11 [継承]
(必須): 1 [継承]
(必須): 430 430 [継承]
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年月日 (必須): 西暦 2020年 10月 2日 (令和 2年 10月 2日) [継承]
URL (任意):
DOI (任意): 10.1186/s13287-020-01946-7    (→Scopusで検索) [継承]
PMID (任意): 33008488    (→Scopusで検索) [継承]
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Scopus (任意): 2-s2.0-85092295153 [継承]
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備考 (任意): 1.(英) Article.ELocationID: 10.1186/s13287-020-01946-7  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
4.(英) KeywordList.Keyword: Induced pluripotent stem cells  (日)    [継承]
5.(英) KeywordList.Keyword: Progenitor cells  (日)    [継承]
6.(英) KeywordList.Keyword: Stem cell transplantation  (日)    [継承]
7.(英) KeywordList.Keyword: Tissue regeneration  (日)    [継承]

標準的な表示

和文冊子 ● Naoya Kawakita, Hiroaki Toba, Keiko Miyoshi, Shinichi Sakamoto, Daisuke Matsumoto, Mika Takashima, Mariko Aoyama, Seiya Inoue, Masami Morimoto, Takeshi Nishino, Hiromitsu Takizawa and Akira Tangoku : Bronchioalveolar stem cells derived from mouse-induced pluripotent stem cells promote airway epithelium regeneration, Stem Cell Research & Therapy, Vol.11, No.1, 430, 2020.
欧文冊子 ● Naoya Kawakita, Hiroaki Toba, Keiko Miyoshi, Shinichi Sakamoto, Daisuke Matsumoto, Mika Takashima, Mariko Aoyama, Seiya Inoue, Masami Morimoto, Takeshi Nishino, Hiromitsu Takizawa and Akira Tangoku : Bronchioalveolar stem cells derived from mouse-induced pluripotent stem cells promote airway epithelium regeneration, Stem Cell Research & Therapy, Vol.11, No.1, 430, 2020.

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