『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=373011EID:373011, Map:0, LastModified:2020年12月24日(木) 10:35:44, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[倉園 久生], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Hisatsune Junzo (日) (読)
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[継承]
2. (英) Nakayama Masaaki (日) (読)
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[継承]
3. (英) Isomoto Hajime (日) (読)
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[継承]
4.倉園 久生 ([徳島大学.研究支援・産官学連携センター])
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学籍番号 (推奨):
[継承]
5. (英) Mukaida Naofumi (日) (読)
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[継承]
6. (英) Mukhopadhyay K. Asish (日) (読)
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[継承]
7. (英) Azuma Takeshi (日) (読)
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学籍番号 (推奨):
[継承]
8. (英) Yamaoka Yoshio (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
9. (英) Sap Jan (日) (読)
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学籍番号 (推奨):
[継承]
10. (英) Yamasaki Eiki (日) (読)
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学籍番号 (推奨):
[継承]
11. (英) Yahiro Kinnosuke (日) (読)
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学籍番号 (推奨):
[継承]
12. (英) Moss Joel (日) (読)
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学籍番号 (推奨):
[継承]
13. (英) Hirayama Toshiya (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Molecular characterization of Helicobacter pylori VacA induction of IL-8 in U937 cells reveals a prominent role for p38MAPK in activating transcription factor-2, cAMP response element binding protein, and NF-kappaB activation.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Helicobacter pylori VacA induces multiple effects on susceptible cells, including vacuolation, mitochondrial damage, inhibition of cell growth, and enhanced cyclooxygenase-2 expression. To assess the ability of H. pylori to modulate the production of inflammatory mediators, we examined the mechanisms by which VacA enhanced IL-8 production by promonocytic U937 cells, which demonstrated the greatest VacA-induced IL-8 release of the cells tested. Inhibitors of p38 MAPK (SB203580), ERK1/2 (PD98059), IkappaBalpha ((E)-3-(4-methylphenylsulfonyl)-2-propenenitrile), Ca(2+) entry (SKF96365), and intracellular Ca(2+) channels (dantrolene) blocked VacA-induced IL-8 production. Furthermore, an intracellular Ca(2+) chelator (BAPTA-AM), which inhibited VacA-activated p38 MAPK, caused a dose-dependent reduction in VacA-induced IL-8 secretion by U937 cells, implying a role for intracellular Ca(2+) in mediating activation of MAPK and the canonical NF-kappaB pathway. VacA stimulated translocation of NF-kappaBp65 to the nucleus, consistent with enhancement of IL-8 expression by activation of the NF-kappaB pathway. In addition, small interfering RNA of activating transcription factor (ATF)-2 or CREB, which is a p38MAPK substrate and binds to the AP-1 site of the IL-8 promoter, inhibited VacA-induced IL-8 production. VacA activated an IL-8 promoter containing an NF-IL-6 site, but not a mutated AP-1 or NF-kappaB site, suggesting direct involvement of the ATF-2/CREB binding region or NF-kappaB-binding regions in VacA-induced IL-8 promoter activation. Thus, in U937 cells, VacA directly increases IL-8 production by activation of the p38 MAPK via intracellular Ca(2+) release, leading to activation of the transcription factors, ATF-2, CREB, and NF-kappaB.  (日)    [継承]
キーワード (推奨): 1. (英) Activating Transcription Factor 2 (日) (読) [継承]
2. (英) Active Transport, Cell Nucleus (日) (読) [継承]
3. (英) Bacterial Proteins (日) (読) [継承]
4. (英) Butylated Hydroxyanisole (日) (読) [継承]
5. (英) Cell Nucleus (日) (読) [継承]
6. (英) Cyclic AMP Response Element-Binding Protein (日) (読) [継承]
7. (英) Dantrolene (日) (読) [継承]
8. (英) Egtazic Acid (日) (読) [継承]
9. (英) Enzyme Activation (日) (読) [継承]
10. (英) Helicobacter pylori (日) (読) [継承]
11. (英) Humans (日) (読) [継承]
12. (英) Imidazoles (日) (読) [継承]
13. (英) Interleukin-8 (日) (読) [継承]
14. (英) NF-kappa B (日) (読) [継承]
15. (英) Nitriles (日) (読) [継承]
16. (英) Phosphorylation (日) (読) [継承]
17. (英) Promoter Regions, Genetic (日) (読) [継承]
18. (英) Sulfones (日) (読) [継承]
19. (英) Thapsigargin (日) (読) [継承]
20. (英) U937 Cells (日) (読) [継承]
21. (英) Up-Regulation (日) (読) [継承]
22. (英) p38 Mitogen-Activated Protein Kinases (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Immunology ([The American Association of Immunologists])
(pISSN: 0022-1767, eISSN: 1550-6606)

ISSN (任意): 0022-1767
ISSN: 0022-1767 (pISSN: 0022-1767, eISSN: 1550-6606)
Title: Journal of immunology (Baltimore, Md. : 1950)
Title(ISO): J Immunol
Publisher: American Association of Immunologists
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(必須): 180 [継承]
(必須): 7 [継承]
(必須): 5017 5027 [継承]
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年月日 (必須): 西暦 2008年 4月 1日 (平成 20年 4月 1日) [継承]
URL (任意):
DOI (任意): 10.4049/jimmunol.180.7.5017    (→Scopusで検索) [継承]
PMID (任意): 18354227    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Junzo Hisatsune, Masaaki Nakayama, Hajime Isomoto, Hisao Kurazono, Naofumi Mukaida, Asish K. Mukhopadhyay, Takeshi Azuma, Yoshio Yamaoka, Jan Sap, Eiki Yamasaki, Kinnosuke Yahiro, Joel Moss and Toshiya Hirayama : Molecular characterization of Helicobacter pylori VacA induction of IL-8 in U937 cells reveals a prominent role for p38MAPK in activating transcription factor-2, cAMP response element binding protein, and NF-kappaB activation., The Journal of Immunology, Vol.180, No.7, 5017-5027, 2008.
欧文冊子 ● Junzo Hisatsune, Masaaki Nakayama, Hajime Isomoto, Hisao Kurazono, Naofumi Mukaida, Asish K. Mukhopadhyay, Takeshi Azuma, Yoshio Yamaoka, Jan Sap, Eiki Yamasaki, Kinnosuke Yahiro, Joel Moss and Toshiya Hirayama : Molecular characterization of Helicobacter pylori VacA induction of IL-8 in U937 cells reveals a prominent role for p38MAPK in activating transcription factor-2, cAMP response element binding protein, and NF-kappaB activation., The Journal of Immunology, Vol.180, No.7, 5017-5027, 2008.

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