| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): | □ | 研究
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| ○共著種別 (推奨): | □ | 国内共著 (徳島大学内研究者と国内(学外)研究者との共同研究 (国外研究者を含まない))
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| ○学究種別 (推奨): | □ | 博士前期課程学生による研究報告
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| ○組織 (推奨): | 1. | 徳島大学.大学院医歯薬学研究部 (2015年4月1日〜)
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| 2. | 徳島大学.薬学研究科.創薬科学専攻 (2012年4月1日〜)
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| ○著者 (必須): | 1. | (英) Ueda Masahiro (日) 上田 将弘 (読) うえだ まさひろ
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| ○学籍番号 (推奨): | □ | ****
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| 2. | (英) Komiya Chiaki (日) 小宮 千明 (読) こみや ちあき
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| ○学籍番号 (推奨): | □ | ****
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| 3. | (英) Arii Sayuki (日) 有井 紗由季 (読) ありい さゆき
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| ○学籍番号 (推奨): | □ | ****
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| 4. | (英) Kusumoto Kohshi (日) 楠本 嵩志 (読) くすもと こうし
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| ○学籍番号 (推奨): | □ | ****
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| 5. | 傳田 将也 ([徳島大学.大学院医歯薬学研究部.薬学域.先端薬学教育研究プロジェクト]/[徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.創薬科学系.機能分子合成薬学])
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| 6. | 奥平 桂一郎
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| 7. | 重永 章
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| 8. | 大髙 章 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.創薬科学系.機能分子合成薬学])
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| ○題名 (必須): | □ | (英) Sequence-independent traceless method for preparation of peptide/protein thioesters using CPaseY-mediated hydrazinolysis (日)
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| ○副題 (任意): |
| ○要約 (任意): | □ | (英) Proteins incorporating artificial moieties such as fluorophores and drugs have enjoyed increasing use in chemical biology and drug development research. Preparation of such artificial protein derivatives has relied mainly on native chemical ligation in which peptide/protein thioesters chemoselectively react with N-terminal cysteine peptides to afford protein molecules. The protein thioesters derived from expressed proteins represent thioesters that are very useful for the preparation of artificial proteins by native chemical ligation with synthetic peptides with N-terminal Cys. We recently have developed a traceless thioester-producing protocol using carboxypeptidase Y (CPaseY) which is compatible with an expressed protein. The traceless character is ensured by CPaseY-mediated hydrazinolysis of C-terminal Xaa (X)-Cys-Pro-Leu-OH sequence followed by an auto-processing of the Cys-Pro (CP) dipeptide unit, affording the corresponding X-thioester (X-SR). However, hydrazinolysis of the amide bond in the prolyl leucine junction depends significantly on the nature of X. In the case of hydrophobic X residues, the hydrazinolysis overreacts to give several hydrazides while the reaction of hydrophilic X residues proceeds slowly. In this research, we attempted to develop an X-independent CPaseY-mediated protocol and found that the incorporation of a triple CP sequence into the C-terminal end (X-(CP)-Leu-OH) allows for efficient X-SR formation in a manner that is independent of X. (日)
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| ○キーワード (推奨): | 1. | (英) carboxypeptidase (日) (読)
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| 2. | (英) hydrazinolysis (日) (読)
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| 3. | (英) native chemical ligation (日) (読)
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| 4. | (英) protein semi-synthesis (日) (読)
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| 5. | (英) protein thioester (日) (読)
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| 6. | (英) thioesterification (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Chemical & Pharmaceutical Bulletin ([日本薬学会])
(pISSN: 0009-2363, eISSN: 1347-5223)
| ○ISSN (任意): | □ | 1347-5223
ISSN: 0009-2363
(pISSN: 0009-2363, eISSN: 1347-5223) Title: Chemical & pharmaceutical bulletinTitle(ISO): Chem Pharm Bull (Tokyo)Supplier: 公益社団法人日本薬学会Publisher: Pharmaceutical Society of Japan (NLM Catalog)
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| ○巻 (必須): | □ | 68
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| ○号 (必須): | □ | 12
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| ○頁 (必須): | □ | 1226 1232
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2020年 9月 25日 (令和 2年 9月 25日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1248/cpb.c20-00674 (→Scopusで検索)
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| ○PMID (任意): | □ | 33028801 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1248/cpb.c20-00674 (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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