『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
ID: Pass:

登録内容 (EID=370994)

EID=370994EID:370994, Map:0, LastModified:2020年9月2日(水) 18:53:01, Operator:[橋本 登], Avail:TRUE, Censor:0, Owner:[橋本 登], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.橋本 登 ([徳島大学.大学院医歯薬学研究部.歯学域.先端歯学教育研究プロジェクト]/[徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.組織再生制御学])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Hamamura Kazunori (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Kotani Norihiro (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Furukawa Keiko (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
5. (英) Kaneko Kei (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
6. (英) Honke Koichi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
7. (英) Furukawa Koichi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Proteomic analysis of ganglioside-associated membrane molecules: substantial basis for molecular clustering.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Ganglioside GD3 is specifically expressed in human melanomas, and plays a role in the enhancement of malignant phenotypes of melanoma cells. To analyze the mechanisms by which GD3 enhances malignant properties and signals in melanomas, it is essential to clarify how GD3 interacts with membrane molecules on the cell membrane. In this study, we performed proteomics analysis of glycolipid-enriched microdomains (GEM) with current sucrose density gradient ultracentrifugation of Triton X-100 extracts and MS. We also examined GD3-associated molecules using enzyme-mediated activation of radical sources (EMARS) reaction combined with MS. Comparison of molecules identified as residents in GEM/rafts and those detected by EMARS reaction using an anti-GD3 antibody revealed that a relatively low number of molecules is recruited around GD3, while a number of membrane and secreted molecules was defined in GEM/rafts. These results suggested that EMARS reaction is useful to identify actually interacting molecules with gangliosides such as GD3 on the cell membrane, and many other microdomains than GD3-associating rafts exist. Representative examples of GD3-associated molecules such as neogenin and MCAM were shown.  (日)    [継承]
キーワード (推奨): 1. (英) Cell Line, Tumor (日) (読) [継承]
2. (英) Gangliosides (日) (読) [継承]
3. (英) Humans (日) (読) [継承]
4.質量分析法 (mass spectrometry) [継承]
5. (英) Melanoma (日) (読) [継承]
6. (英) Membrane Microdomains (日) (読) [継承]
7. (英) Membrane Proteins (日) (読) [継承]
8.プロテオーム (proteome) [継承]
9.プロテオミクス (proteomics) [継承]
発行所 (推奨):
誌名 (必須): Proteomics ([Wiley-VCH])
(pISSN: 1615-9853, eISSN: 1615-9861)

ISSN (任意): 1615-9861
ISSN: 1615-9853 (pISSN: 1615-9853, eISSN: 1615-9861)
Title: Proteomics
Title(ISO): Proteomics
Publisher: Wiley Publishing
 (NLM Catalog  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 12 [継承]
(必須): 21 [継承]
(必須): 3154 3163 [継承]
都市 (任意):
年月日 (必須): 西暦 2012年 10月 1日 (平成 24年 10月 1日) [継承]
URL (任意):
DOI (任意): 10.1002/pmic.201200279    (→Scopusで検索) [継承]
PMID (任意): 22936677    (→Scopusで検索) [継承]
CRID (任意):
WOS (任意):
Scopus (任意):
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意): 1.(英) PublicationType: Journal Article  (日)    [継承]
2.(英) PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Noboru Hashimoto, Kazunori Hamamura, Norihiro Kotani, Keiko Furukawa, Kei Kaneko, Koichi Honke and Koichi Furukawa : Proteomic analysis of ganglioside-associated membrane molecules: substantial basis for molecular clustering., Proteomics, Vol.12, No.21, 3154-3163, 2012.
欧文冊子 ● Noboru Hashimoto, Kazunori Hamamura, Norihiro Kotani, Keiko Furukawa, Kei Kaneko, Koichi Honke and Koichi Furukawa : Proteomic analysis of ganglioside-associated membrane molecules: substantial basis for molecular clustering., Proteomics, Vol.12, No.21, 3154-3163, 2012.

関連情報

Number of session users = 1, LA = 0.79, Max(EID) = 406903, Max(EOID) = 1102773.