『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Bhuiyan Robiul Hasan (日) (読)
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[継承]
2. (英) Kondo Yuji (日) (読)
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学籍番号 (推奨):
[継承]
3. (英) Yamaguchi Tokiaki (日) (読)
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学籍番号 (推奨):
[継承]
4. (英) Tokuda Noriyo (日) (読)
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学籍番号 (推奨):
[継承]
5. (英) Ohkawa Yuki (日) (読)
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学籍番号 (推奨):
[継承]
6.橋本 登 ([徳島大学.大学院医歯薬学研究部.歯学域.先端歯学教育研究プロジェクト]/[徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.組織再生制御学])
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学籍番号 (推奨):
[継承]
7. (英) Ohmi Yuhsuke (日) (読)
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学籍番号 (推奨):
[継承]
8. (英) Yamauchi Yoshio (日) (読)
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学籍番号 (推奨):
[継承]
9. (英) Furukawa Keiko (日) (読)
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学籍番号 (推奨):
[継承]
10. (英) Okajima Tetsuya (日) (読)
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[継承]
11. (英) Furukawa Koichi (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Expression analysis of 0-series gangliosides in human cancer cell lines with monoclonal antibodies generated using knockout mice of ganglioside synthase genes.  (日)    [継承]
副題 (任意):
要約 (任意): (英)   (日) Some gangliosides, sialic acid-containing glycosphingolipids, have been considered as tumor-associated antigens. GD1α or a GD1α synthase gene ST6GalNAc5 was reported to be involved in the metastasis of murine lymphomas or human breast cancers, respectively. But expression patterns of 0-series gangliosides GD1α and its precursor GM1b in human cancers have not yet been investigated mainly due to lack of specific antibodies. We established specific monoclonal antibodies (mAbs) reactive with GD1α or GM1b using gangliosides from brain tissues of GM3 synthase (St3gal5)-deficient mice as immunogens. We used GM2/GD2 synthase (B4galnt1)-deficient mice to immunize by liposomes embedded with GD1α or acidic glycolipid fractions from brain of St3gal5-deficient mice. Specificities of established mAbs as analyzed by enzyme-linked immunosorbent assay and thin-layer chromatography-immunostaining were very high among various gangliosides. Increased expression of GD1α and reduced GM1b in the St6galnac5 cDNA-transfected RAW117 cell line also substantiated the specificities of two mAbs. Then, we analyzed expression of GD1α and GM1b, and of relevant glycosyltransferase genes in various human cancer cell lines using generated anti-GD1α mAb 122 or anti-GM1b mAb MR155A-7. A few human cancer cell lines showed significant expression of these gangliosides with reasonable expression of relevant glycosyltransferase genes.   [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Antibodies, Monoclonal (日) (読) [継承]
3. (英) Antigens, Neoplasm (日) (読) [継承]
4. (英) Cell Line, Tumor (日) (読) [継承]
5. (英) G(M1) Ganglioside (日) (読) [継承]
6. (英) Gangliosides (日) (読) [継承]
7. (英) Gene Expression Regulation, Enzymologic (日) (読) [継承]
8. (英) Glycosphingolipids (日) (読) [継承]
9. (英) Humans (日) (読) [継承]
10.ノックアウトマウス (knockout mice) [継承]
11. (英) N-Acetylgalactosaminyltransferases (日) (読) [継承]
12. (英) Neoplasm Metastasis (日) (読) [継承]
13. (英) Sialyltransferases (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Glycobiology (Society for Glycobiology)
(pISSN: 0959-6658, eISSN: 1460-2423)

ISSN (任意): 1460-2423
ISSN: 0959-6658 (pISSN: 0959-6658, eISSN: 1460-2423)
Title: Glycobiology
Title(ISO): Glycobiology
Supplier: Oxford University Press
Publisher: Oxford University Press
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 26 [継承]
(必須): 9 [継承]
(必須): 984 998 [継承]
都市 (任意):
年月日 (必須): 西暦 2016年 4月 21日 (平成 28年 4月 21日) [継承]
URL (任意):
DOI (任意): 10.1093/glycob/cww049    (→Scopusで検索) [継承]
PMID (任意): 27102283    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) PublicationType: Journal Article  (日)    [継承]
2.(英) PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Hasan Robiul Bhuiyan, Yuji Kondo, Tokiaki Yamaguchi, Noriyo Tokuda, Yuki Ohkawa, Noboru Hashimoto, Yuhsuke Ohmi, Yoshio Yamauchi, Keiko Furukawa, Tetsuya Okajima and Koichi Furukawa : Expression analysis of 0-series gangliosides in human cancer cell lines with monoclonal antibodies generated using knockout mice of ganglioside synthase genes., Glycobiology, Vol.26, No.9, 984-998, 2016.
欧文冊子 ● Hasan Robiul Bhuiyan, Yuji Kondo, Tokiaki Yamaguchi, Noriyo Tokuda, Yuki Ohkawa, Noboru Hashimoto, Yuhsuke Ohmi, Yoshio Yamauchi, Keiko Furukawa, Tetsuya Okajima and Koichi Furukawa : Expression analysis of 0-series gangliosides in human cancer cell lines with monoclonal antibodies generated using knockout mice of ganglioside synthase genes., Glycobiology, Vol.26, No.9, 984-998, 2016.

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