『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=370986EID:370986, Map:0, LastModified:2020年9月2日(水) 18:43:12, Operator:[橋本 登], Avail:TRUE, Censor:0, Owner:[橋本 登], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Kaneko Kei (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) Ohkawa Yuki (日) (読)
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学籍番号 (推奨):
[継承]
3.橋本 登 ([徳島大学.大学院医歯薬学研究部.歯学域.先端歯学教育研究プロジェクト]/[徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.組織再生制御学])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Ohmi Yuhsuke (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
5. (英) Kotani Norihiro (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
6. (英) Honke Koichi (日) (読)
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学籍番号 (推奨):
[継承]
7. (英) Ogawa Mitsutaka (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
8. (英) Okajima Tetsuya (日) (読)
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学籍番号 (推奨):
[継承]
9. (英) Furukawa Keiko (日) (読)
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貢献度 (任意):
学籍番号 (推奨):
[継承]
10. (英) Furukawa Koichi (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Neogenin, Defined as a GD3-associated Molecule by Enzyme-mediated Activation of Radical Sources, Confers Malignant Properties via Intracytoplasmic Domain in Melanoma Cells.  (日)    [継承]
副題 (任意):
要約 (任意): (英) To investigate mechanisms for increased malignant properties in malignant melanomas by ganglioside GD3, enzyme-mediated activation of radical sources and subsequent mass spectrometry were performed using an anti-GD3 antibody and GD3-positive (GD3+) and GD3-negative (GD3-) melanoma cell lines. Neogenin, defined as a GD3-neighbored molecule, was largely localized in lipid/rafts in GD3+ cells. Silencing of neogenin resulted in the reduction of cell growth and invasion activity. Physical association between GD3 and neogenin was demonstrated by immunoblotting of the immunoprecipitates with anti-neogenin antibody from GD3+ cell lysates. The intracytoplasmic domain of neogenin (Ne-ICD) was detected in GD3+ cells at higher levels than in GD3- cells when cells were treated by a proteasome inhibitor but not when simultaneously treated with a γ-secretase inhibitor. Exogenous GD3 also induced increased Ne-ICD in GD3- cells. Overexpression of Ne-ICD in GD3- cells resulted in the increased cell growth and invasion activity, suggesting that Ne-ICD plays a role as a transcriptional factor to drive malignant properties of melanomas after cleavage with γ-secretase. γ-Secretase was found in lipid/rafts in GD3+ cells. Accordingly, immunocyto-staining revealed that GD3, neogenin, and γ-secretase were co-localized at the leading edge of GD3+ cells. All these results suggested that GD3 recruits γ-secretase to lipid/rafts, allowing efficient cleavage of neogenin. ChIP-sequencing was performed to identify candidates of target genes of Ne-ICD. Some of them actually showed increased expression after expression of Ne-ICD, probably exerting malignant phenotypes of melanomas under GD3 expression.  (日)    [継承]
キーワード (推奨): 1. (英) Amyloid Precursor Protein Secretases (日) (読) [継承]
2. (英) Cell Line, Tumor (日) (読) [継承]
3. (英) Gangliosides (日) (読) [継承]
4. (英) Gene Expression Regulation, Neoplastic (日) (読) [継承]
5. (英) Humans (日) (読) [継承]
6. (英) Melanoma (日) (読) [継承]
7. (英) Membrane Microdomains (日) (読) [継承]
8. (英) Neoplasm Proteins (日) (読) [継承]
9. (英) Nerve Tissue Proteins (日) (読) [継承]
10. (英) Receptors, Cell Surface (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Biological Chemistry ([The American Society for Biochemistry and Molecular Biology])
(pISSN: 0021-9258, eISSN: 1083-351X)

ISSN (任意): 1083-351X
ISSN: 0021-9258 (pISSN: 0021-9258, eISSN: 1083-351X)
Title: The Journal of biological chemistry
Title(ISO): J Biol Chem
Publisher: American Society for Biochemistry and Molecular Biology
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 291 [継承]
(必須): 32 [継承]
(必須): 16630 16643 [継承]
都市 (任意):
年月日 (必須): 西暦 2016年 6月 10日 (平成 28年 6月 10日) [継承]
URL (任意):
DOI (任意): 10.1074/jbc.M115.708834    (→Scopusで検索) [継承]
PMID (任意): 27288875    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) PublicationType: Journal Article  (日)    [継承]
2.(英) PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Kei Kaneko, Yuki Ohkawa, Noboru Hashimoto, Yuhsuke Ohmi, Norihiro Kotani, Koichi Honke, Mitsutaka Ogawa, Tetsuya Okajima, Keiko Furukawa and Koichi Furukawa : Neogenin, Defined as a GD3-associated Molecule by Enzyme-mediated Activation of Radical Sources, Confers Malignant Properties via Intracytoplasmic Domain in Melanoma Cells., The Journal of Biological Chemistry, Vol.291, No.32, 16630-16643, 2016.
欧文冊子 ● Kei Kaneko, Yuki Ohkawa, Noboru Hashimoto, Yuhsuke Ohmi, Norihiro Kotani, Koichi Honke, Mitsutaka Ogawa, Tetsuya Okajima, Keiko Furukawa and Koichi Furukawa : Neogenin, Defined as a GD3-associated Molecule by Enzyme-mediated Activation of Radical Sources, Confers Malignant Properties via Intracytoplasmic Domain in Melanoma Cells., The Journal of Biological Chemistry, Vol.291, No.32, 16630-16643, 2016.

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