『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=370525EID:370525, Map:0, LastModified:2020年9月2日(水) 19:37:41, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[笠原 二郎], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.三宅 一央
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[継承]
2.坂根 亜由子 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.生理系.生化学])
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3.佐川 幾子 ([徳島大学.大学院医歯薬学研究部.医学域.総合研究支援センター])
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4. (英) (日) 富田 陽子 (読)
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5.笠原 二郎 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.生命薬学系.神経病態解析学])
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6.佐々木 卓也 ([徳島大学])
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題名 (必須): (英) Actin Cytoskeletal Reorganization Function of JRAB/MICAL-L2 Is Fine-tuned by Intramolecular Interaction between First LIM Zinc Finger and C-terminal Coiled-coil Domains  (日)    [継承]
副題 (任意):
要約 (任意): (英) JRAB/MICAL-L2 is an effector protein of Rab13, a member of the Rab family of small GTPase. JRAB/MICAL-L2 consists of a calponin homology domain, a LIM domain, and a coiled-coil domain. JRAB/MICAL-L2 engages in intramolecular interaction between the N-terminal LIM domain and the C-terminal coiled-coil domain, and changes its conformation from closed to open under the effect of Rab13. Open-form JRAB/MICAL-L2 induces the formation of peripheral ruffles via an interaction between its calponin homology domain and filamin. Here, we report that the LIM domain, independent of the C-terminus, is also necessary for the function of open-form JRAB/MICAL-L2. In mechanistic terms, two zinc finger domains within the LIM domain bind the first and second molecules of actin at the minus end, potentially inhibiting the depolymerization of actin filaments (F-actin). The first zinc finger domain also contributes to the intramolecular interaction of JRAB/MICAL-L2. Moreover, the residues of the first zinc finger domain that are responsible for the intramolecular interaction are also involved in the association with F-actin. Together, our findings show that the function of open-form JRAB/MICAL-L2 mediated by the LIM domain is fine-tuned by the intramolecular interaction between the first zinc finger domain and the C-terminal domain.  (日)    [継承]
キーワード (推奨): 1. (英) 3T3 Cells (日) (読) [継承]
2. (英) Actins (日) (読) [継承]
3. (英) Animals (日) (読) [継承]
4. (英) Cytoskeleton (日) (読) [継承]
5. (英) Hydrogen Deuterium Exchange-Mass Spectrometry (日) (読) [継承]
6. (英) Mice (日) (読) [継承]
7. (英) Microfilament Proteins (日) (読) [継承]
8. (英) Models, Molecular (日) (読) [継承]
9. (英) Mutation (日) (読) [継承]
10. (英) Protein Domains (日) (読) [継承]
11. (英) Structure-Activity Relationship (日) (読) [継承]
12. (英) Zinc Fingers (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Scientific Reports ([Nature Publishing Group])
(eISSN: 2045-2322)

ISSN (任意): 2045-2322
ISSN: 2045-2322 (eISSN: 2045-2322)
Title: Scientific reports
Title(ISO): Sci Rep
Publisher: Springer Science+Business Media
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 9 [継承]
(必須): 1 [継承]
(必須): 12794 12794 [継承]
都市 (任意):
年月日 (必須): 西暦 2019年 9月 5日 (令和 元年 9月 5日) [継承]
URL (任意):
DOI (任意): 10.1038/s41598-019-49232-8    (→Scopusで検索) [継承]
PMID (任意): 31488862    (→Scopusで検索) [継承]
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Scopus (任意): 2-s2.0-85071764631 [継承]
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備考 (任意): 1.(英) Article.ELocationID: 10.1038/s41598-019-49232-8  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Kazuhisa Miyake, Ayuko Sakane, Ikuko Sagawa, 富田 陽子, Jiro Kasahara and Takuya Sasaki : Actin Cytoskeletal Reorganization Function of JRAB/MICAL-L2 Is Fine-tuned by Intramolecular Interaction between First LIM Zinc Finger and C-terminal Coiled-coil Domains, Scientific Reports, Vol.9, No.1, 12794, 2019.
欧文冊子 ● Kazuhisa Miyake, Ayuko Sakane, Ikuko Sagawa, 富田 陽子, Jiro Kasahara and Takuya Sasaki : Actin Cytoskeletal Reorganization Function of JRAB/MICAL-L2 Is Fine-tuned by Intramolecular Interaction between First LIM Zinc Finger and C-terminal Coiled-coil Domains, Scientific Reports, Vol.9, No.1, 12794, 2019.

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