| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): | □ | Peer Review
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| ○カテゴリ (推奨): | □ | 研究
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| ○共著種別 (推奨): |
| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Farahat Mahmoud (日) (読)
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| 2. | (英) Kazi A.S. Gulsan (日) (読)
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| 3. | (英) Taketa Hiroaki (日) (読)
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| 4. | (英) Hara S. Emilio (日) (読)
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| 5. | 大島 正充 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.顎機能咬合再建学])
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| 6. | (英) Kuboki Takuo (日) (読)
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| 7. | (英) Matsumoto Takuya (日) (読)
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| ○題名 (必須): | □ | (英) Fibronectin-induced ductal formation in salivary gland self-organization model (日)
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| ○要約 (任意): | □ | (英) Recent advances in tissue regeneration approaches including 3D organoids, were based on various 3D organogenesis models. However, 3D models are generally technique-sensitive and time-consuming. Thus, we utilized an existing model of submandibular salivary gland (SMG) to modify a simple and highly reproducible in vitro 3D culture model of primary SMG cells self-organization into a well-developed cell spheroid inside Matrigel substrate. We used this model to observe the collective multicellular behavior during spheroid formation. Further, we applied various quantitative approaches including real-time live imaging and immune histochemical image analysis to dissect the cellular dynamics during tissue patterning. On a time-scale of hours, we observed marked size and shape transformations in the developed 3D spheroid which resulted in a spatially-controlled growth differential from the canter to the periphery of the formed aggregates. Moreover, we investigated the effect of fibronectin (FN) on SMG cells self-organization using our simplified culture model. Interestingly, we discovered a novel role of FN in inducing duct-like elongation during initial stages of SMG bud formation. This in vitro model provides an excellent tool for analyzing the intercellular dynamics during early SMG tissue development as well as revealing a novel role of FN in SMG ductal expansion. (日)
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| ○キーワード (推奨): | 1. | (英) Animals (日) (読)
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| 2. | (英) Cell Culture Techniques (日) (読)
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| 3. | (英) Cells, Cultured (日) (読)
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| 4. | コラーゲン (collagen)
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| 5. | (英) Drug Combinations (日) (読)
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| 6. | (英) Fibronectins (日) (読)
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| 7. | (英) Laminin (日) (読)
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| 8. | (英) Mice (日) (読)
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| 9. | (英) Organogenesis (日) (読)
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| 10. | (英) Proteoglycans (日) (読)
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| 11. | (英) Salivary Ducts (日) (読)
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| 12. | 唾液腺 (salivary glands/->キーワード[唾液腺])
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| 13. | (英) Spheroids, Cellular (日) (読)
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| 14. | (英) Submandibular Gland (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Developmental Dynamics (American Association of Anatomists)
(pISSN: 1058-8388, eISSN: 1097-0177)
| ○ISSN (任意): | □ | 1097-0177
ISSN: 1058-8388
(pISSN: 1058-8388, eISSN: 1097-0177) Title: Developmental dynamics : an official publication of the American Association of AnatomistsTitle(ISO): Dev DynSupplier: American Association for AnatomyPublisher: Wiley (NLM Catalog)
(Wiley)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 248
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| ○号 (必須): | □ | 9
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| ○頁 (必須): | □ | 813 825
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2019年 9月 初日 (令和 元年 9月 初日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1002/dvdy.78 (→Scopusで検索)
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| ○PMID (任意): | □ | 31237723 (→Scopusで検索)
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| ○CRID (任意): |
| ○WOS (任意): |
| ○Scopus (任意): | □ | 2-s2.0-85069903335
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| ○評価値 (任意): |
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| ○指導教員 (推奨): |
| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1002/dvdy.78 (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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| 4. | (英) KeywordList.Keyword: 3D organoids (日)
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| 5. | (英) KeywordList.Keyword: ductal formation (日)
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| 6. | (英) KeywordList.Keyword: extracellular matrix (日)
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| 7. | (英) KeywordList.Keyword: fibronectin (日)
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| 8. | (英) KeywordList.Keyword: salivary gland (日)
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| 9. | (英) KeywordList.Keyword: self-organization (日)
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