| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○カテゴリ (推奨): |
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| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Hashimoto Muneaki (日) (読)
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| 2. | 板東 美香 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.臨床歯学系.歯周歯内治療学]/[徳島大学.病院.診療科.歯科.歯周病科(第二保存科)])
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| 3. | 木戸 淳一
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| 4. | (英) Yokota Kazumichi (日) (読)
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| 5. | (英) Mita Toshihiro (日) (読)
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| 6. | (英) Kajimoto Kazuaki (日) (読)
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| 7. | 片岡 正俊
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| ○題名 (必須): | □ | (英) Nucleic acid purification from dried blood spot on FTA elute card provides template for polymerase chain reaction for highly sensitive Plasmodium detection (日)
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| ○要約 (任意): | □ | (英) Polymerase chain reaction (PCR) is an essential diagnostic method for highly sensitive detection of Plasmodium-infected erythrocytes in patients with malaria. This study compared the performance of filter papers used for the preparation of dried blood spots (DBS) in detecting Plasmodium by PCR. Whole blood spiked with P. falciparum-infected erythrocytes to obtain samples with various levels of parasitemia were applied to Whatman 3MM Chr papers, FTA Cards, or FTA Elute Cards to prepare the DBS. DNA was purified from the DBS using a DNA purification kit and used as the template for nested PCR. In probit analysis, the estimated limit of detection (LoD) was 5.5 parasites/μL blood for Whatman 3MM Chr papers and FTA Cards and 1.6 parasites/μL blood for the FTA Elute Card. This result suggested that the DBS prepared on an FTA Elute Card yield the best template DNA for subsequent high-sensitivity PCR-based detection of P. falciparum-infected erythrocytes. This finding can help improve the accuracy of malarial diagnostic tests. (日)
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| ○キーワード (推奨): | 1. | (英) DNA, Protozoan (日) (読)
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| 2. | (英) Diagnostic Tests, Routine (日) (読)
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| 3. | (英) Dried Blood Spot Testing (日) (読)
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| 4. | (英) Humans (日) (読)
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| 5. | (英) Limit of Detection (日) (読)
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| 6. | (英) Malaria, Falciparum (日) (読)
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| 7. | (英) Plasmodium falciparum (日) (読)
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| 8. | (英) Polymerase Chain Reaction (日) (読)
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| 9. | (英) RNA, Ribosomal, 18S (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Parasitology International ([日本寄生虫学会])
(pISSN: 1383-5769, eISSN: 1873-0329)
| ○ISSN (任意): | □ | 1873-0329
ISSN: 1383-5769
(pISSN: 1383-5769, eISSN: 1873-0329) Title: Parasitology internationalTitle(ISO): Parasitol IntPublisher: Elsevier Ireland Ltd (NLM Catalog)
(医中誌Web)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 73
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| ○号 (必須): | □ |
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| ○頁 (必須): | □ | 101941 101941
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| ○年月日 (必須): | □ | 西暦 2019年 8月 20日 (令和 元年 8月 20日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1016/j.parint.2019.101941 (→Scopusで検索)
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| ○PMID (任意): | □ | 31442664 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.ELocationID: S1383-5769(19)30137-0 (日)
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| 2. | (英) Article.ELocationID: 10.1016/j.parint.2019.101941 (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Comparative Study (日)
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| 4. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 5. | (英) KeywordList.Keyword: Dried blood spots (日)
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| 6. | (英) KeywordList.Keyword: FTA Elute Card (日)
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| 7. | (英) KeywordList.Keyword: Limit of detection (日)
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| 8. | (英) KeywordList.Keyword: Malaria (日)
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| 9. | (英) KeywordList.Keyword: PCR (日)
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