『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=365396EID:365396, Map:0, LastModified:2020年7月31日(金) 14:18:59, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[吉田 賀弥], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨): 国際共著 (徳島大学内研究者と国外研究機関所属研究者との共同研究) [継承]
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Okusha Yuka (日) (読)
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[継承]
2. (英) Eguchi Takanori (日) (読)
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[継承]
3. (英) Tran T Manh (日) (読)
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[継承]
4. (英) Sogawa Chiharu (日) (読)
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5.吉田 賀弥 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.口腔保健学系.口腔保健教育学]/[徳島大学.歯学部.口腔保健学科.口腔保健基礎学講座])
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[継承]
6. (英) Itagaki Mami (日) (読)
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[継承]
7. (英) Taha A Eman (日) (読)
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[継承]
8. (英) Ono Kisho (日) (読)
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9. (英) Aoyama Eriko (日) (読)
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10.岡村 裕彦
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11. (英) Kozaki Ken-ichi (日) (読)
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12. (英) Calderwood K Stuart (日) (読)
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13. (英) Takigawa Masaharu (日) (読)
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14. (英) Okamoto Kuniaki (日) (読)
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題名 (必須): (英) Extracellular Vesicles Enriched With Moonlighting Metalloproteinase Are Highly Transmissive, Pro-Tumorigenic, and Trans-Activates Cellular Communication Network Factor ( CCN2/CTGF): CRISPR Against Cancer  (日)    [継承]
副題 (任意):
要約 (任意): (英) Matrix metalloproteinase 3 (MMP3) plays multiple roles in extracellular proteolysis as well as intracellular transcription, prompting a new definition of moonlighting metalloproteinase (MMP), according to a definition of protein moonlighting (or gene sharing), a phenomenon by which a protein can perform more than one function. Indeed, connective tissue growth factor (CTGF, aka cellular communication network factor 2 (CCN2)) is transcriptionally induced as well as cleaved by MMP3. Moreover, several members of the MMP family have been found within tumor-derived extracellular vesicles (EVs). We here investigated the roles of MMP3-rich EVs in tumor progression, molecular transmission, and gene regulation. EVs derived from a rapidly metastatic cancer cell line (LuM1) were enriched in MMP3 and a C-terminal half fragment of CCN2/CTGF. MMP3-rich, LuM1-derived EVs were disseminated to multiple organs through body fluid and were pro-tumorigenic in an allograft mouse model, which prompted us to define LuM1-EVs as oncosomes in the present study. Oncosome-derived MMP3 was transferred into recipient cell nuclei and thereby trans-activated the promoter, and induced CCN2/CTGF production in vitro. TRENDIC and other cis-elements in the promoter were essential for the oncosomal responsivity. The CRISPR/Cas9-mediated knockout of MMP3 showed significant anti-tumor effects such as the inhibition of migration and invasion of tumor cells, and a reduction in CCN2/CTGF promoter activity and fragmentations in vitro. A high expression level of MMP3 or CCN2/CTGF mRNA was prognostic and unfavorable in particular types of cancers including head and neck, lung, pancreatic, cervical, stomach, and urothelial cancers. These data newly demonstrate that oncogenic EVs-derived MMP is a transmissive trans-activator for the cellular communication network gene and promotes tumorigenesis at distant sites.  (日)    [継承]
キーワード (推奨):
発行所 (推奨):
誌名 (必須): Cancers (Basel)
(eISSN: 2072-6694)

ISSN (任意): 2072-6694
ISSN: 2072-6694 (eISSN: 2072-6694)
Title: Cancers
Title(ISO): Cancers (Basel)
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 12 [継承]
(必須): (4) [継承]
(必須): E881 E881 [継承]
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年月日 (必須): 西暦 2020年 4月 4日 (令和 2年 4月 4日) [継承]
URL (任意):
DOI (任意): 10.3390/cancers12040881    (→Scopusで検索) [継承]
PMID (任意): 32260433    (→Scopusで検索) [継承]
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Scopus (任意): 2-s2.0-85083239499 [継承]
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備考 (任意): 1.(英) Article.ELocationID: E881  (日)    [継承]
2.(英) Article.ELocationID: 10.3390/cancers12040881  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
4.(英) KeywordList.Keyword: CCN2/CTGF  (日)    [継承]
5.(英) KeywordList.Keyword: CRISPR  (日)    [継承]
6.(英) KeywordList.Keyword: cellular communication network factor  (日)    [継承]
7.(英) KeywordList.Keyword: extracellular vesicles  (日)    [継承]
8.(英) KeywordList.Keyword: genome editing  (日)    [継承]
9.(英) KeywordList.Keyword: matrix metalloproteinase  (日)    [継承]
10.(英) KeywordList.Keyword: moonlighting metalloproteinase (MMP)  (日)    [継承]
11.(英) KeywordList.Keyword: oncosome  (日)    [継承]
12.(英) KeywordList.Keyword: protein moonlighting  (日)    [継承]
13.(英) KeywordList.Keyword: transcription factor  (日)    [継承]

標準的な表示

和文冊子 ● Yuka Okusha, Takanori Eguchi, Manh T Tran, Chiharu Sogawa, Kaya Yoshida, Mami Itagaki, Eman A Taha, Kisho Ono, Eriko Aoyama, Hirohiko Okamura, Ken-ichi Kozaki, Stuart K Calderwood, Masaharu Takigawa and Kuniaki Okamoto : Extracellular Vesicles Enriched With Moonlighting Metalloproteinase Are Highly Transmissive, Pro-Tumorigenic, and Trans-Activates Cellular Communication Network Factor ( CCN2/CTGF): CRISPR Against Cancer, Cancers, Vol.12, No.(4), E881, 2020.
欧文冊子 ● Yuka Okusha, Takanori Eguchi, Manh T Tran, Chiharu Sogawa, Kaya Yoshida, Mami Itagaki, Eman A Taha, Kisho Ono, Eriko Aoyama, Hirohiko Okamura, Ken-ichi Kozaki, Stuart K Calderwood, Masaharu Takigawa and Kuniaki Okamoto : Extracellular Vesicles Enriched With Moonlighting Metalloproteinase Are Highly Transmissive, Pro-Tumorigenic, and Trans-Activates Cellular Communication Network Factor ( CCN2/CTGF): CRISPR Against Cancer, Cancers, Vol.12, No.(4), E881, 2020.

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