| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Konishi S (日) (読)
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| 2. | (英) Yano T (日) (読)
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| 3. | (英) Tanaka H (日) (読)
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| 4. | (英) Mizuno T (日) (読)
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| 5. | (英) Kanoh H (日) (読)
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| 6. | (英) Tsukita K (日) (読)
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| 7. | (英) Namba T (日) (読)
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| 8. | (英) Tamura A (日) (読)
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| 9. | 米村 重信 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.生理系.細胞生物学])
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| 10. | (英) Gotoh S (日) (読)
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| 11. | (英) Matsumoto H (日) (読)
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| 12. | (英) Hirai T (日) (読)
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| 13. | (英) Tsukita S (日) (読)
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| ○題名 (必須): | □ | (英) Vinculin is critical for the robustness of the epithelial cell sheet paracellular barrier for ions. (日)
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| ○要約 (任意): | □ | (英) The paracellular barrier function of tight junctions (TJs) in epithelial cell sheets is robustly maintained against mechanical fluctuations, by molecular mechanisms that are poorly understood. Vinculin is an adaptor of a mechanosensory complex at the adherens junction. Here, we generated vinculin KO Eph4 epithelial cells and analyzed their confluent cell-sheet properties. We found that vinculin is dispensable for the basic TJ structural integrity and the paracellular barrier function for larger solutes. However, vinculin is indispensable for the paracellular barrier function for ions. In addition, TJs stochastically showed dynamically distorted patterns in vinculin KO cell sheets. These KO phenotypes were rescued by transfecting full-length vinculin and by relaxing the actomyosin tension with blebbistatin, a myosin II ATPase activity inhibitor. Our findings indicate that vinculin resists mechanical fluctuations to maintain the TJ paracellular barrier function for ions in epithelial cell sheets. (日)
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| ○キーワード (推奨): | 1. | (英) Actomyosin (日) (読)
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| 2. | (英) Cell Line (日) (読)
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| 3. | (英) Epithelial Cells (日) (読)
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| 4. | (英) Gene Knockout Techniques (日) (読)
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| 5. | (英) HEK293 Cells (日) (読)
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| 6. | (英) Heterocyclic Compounds, 4 or More Rings (日) (読)
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| 7. | (英) Humans (日) (読)
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| 8. | (英) Ions (日) (読)
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| 9. | (英) Stochastic Processes (日) (読)
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| 10. | (英) Tight Junctions (日) (読)
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| 11. | (英) Vinculin (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Life Science Alliance (Life Science Alliance LLC/[The Rockefeller University Press]/Cold Spring Harbor Laboratory Press)
(eISSN: 2575-1077)
| ○ISSN (任意): | □ | 2575-1077
ISSN: 2575-1077
(eISSN: 2575-1077) Title: Life science allianceTitle(ISO): Life Sci AlliancePublisher: Life Science Alliance, LLC (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 2
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| ○号 (必須): | □ | 4
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| ○頁 (必須): |
| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2019年 8月 9日 (令和 元年 8月 9日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.26508/lsa.201900414 (→Scopusで検索)
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| ○PMID (任意): | □ | 31399484 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.ELocationID: e201900414 (日)
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| 2. | (英) Article.ELocationID: 10.26508/lsa.201900414 (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 4. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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