| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○カテゴリ (推奨): |
| ○共著種別 (推奨): | □ | 徳島大学以外での国際共著 (徳島大学以外での複数の国の研究機関による共同研究)
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Tsujioka Masatsune (日) (読)
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| 2. | (英) Uyeda QP Taro (日) (読)
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| 3. | (英) Iwadate Yoshiaki (日) (読)
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| 4. | (英) Patel Hitesh (日) (読)
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| 5. | (英) Shibata Keitaro (日) (読)
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| 6. | (英) Yumoto Tenji (日) (読)
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| 7. | 米村 重信 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.生理系.細胞生物学])
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| ○題名 (必須): | □ | (英) Actin-binding domains mediate the distinct distribution of two Dictyostelium Talins through different affinities to specific subsets of actin filaments during directed cell migration. (日)
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| ○要約 (任意): | □ | (英) Although the distinct distribution of certain molecules along the anterior or posterior edge is essential for directed cell migration, the mechanisms to maintain asymmetric protein localization have not yet been fully elucidated. Here, we studied a mechanism for the distinct localizations of two Dictyostelium talin homologues, talin A and talin B, both of which play important roles in cell migration and adhesion. Using GFP fusion, we found that talin B, as well as its C-terminal actin-binding region, which consists of an I/LWEQ domain and a villin headpiece domain, was restricted to the leading edge of migrating cells. This is in sharp contrast to talin A and its C-terminal actin-binding domain, which co-localized with myosin II along the cell posterior cortex, as reported previously. Intriguingly, even in myosin II-null cells, talin A and its actin-binding domain displayed a specific distribution, co-localizing with stretched actin filaments. In contrast, talin B was excluded from regions rich in stretched actin filaments, although a certain amount of its actin-binding region alone was present in those areas. When cells were sucked by a micro-pipette, talin B was not detected in the retracting aspirated lobe where acto-myosin, talin A, and the actin-binding regions of talin A and talin B accumulated. Based on these results, we suggest that talin A predominantly interacts with actin filaments stretched by myosin II through its C-terminal actin-binding region, while the actin-binding region of talin B does not make such distinctions. Furthermore, talin B appears to have an additional, unidentified mechanism that excludes it from the region rich in stretched actin filaments. We propose that these actin-binding properties play important roles in the anterior and posterior enrichment of talin B and talin A, respectively, during directed cell migration. (日)
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| ○キーワード (推奨): |
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| ○誌名 (必須): | □ | PLoS ONE (Public Library of Science)
(eISSN: 1932-6203)
| ○ISSN (任意): | □ | 1932-6203
ISSN: 1932-6203
(eISSN: 1932-6203) Title: PloS oneTitle(ISO): PLoS OnePublisher: PLOS (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 14
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| ○号 (必須): | □ | 4
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| ○頁 (必須): | □ | e0214736 e0214736
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2019年 4月 4日 (平成 31年 4月 4日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1371/journal.pone.0214736 (→Scopusで検索)
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| ○PMID (任意): | □ | 30946777 (→Scopusで検索)
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| ○Scopus (任意): | □ | 2-s2.0-85063968594
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| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1371/journal.pone.0214736 (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) CoiStatement: The authors have declared that no competing interests exist. (日)
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