| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○著者 (必須): | 1. | (英) Wakayama Sayaka (日) (読)
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| 2. | (英) Ito Taiyu (日) (読)
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| 3. | (英) Kamada Yuko (日) (読)
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| 4. | 米村 重信 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.生理系.細胞生物学])
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| 5. | (英) Oosa Masatoshi (日) (読)
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| 6. | (英) Kishigami Satoshi (日) (読)
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| 7. | (英) Wakayama Teruhiko (日) (読)
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| ○題名 (必須): | □ | (英) Tolerance of the freeze-dried mouse sperm nucleus to temperatures ranging from -196°C to 150°C. (日)
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| ○要約 (任意): | □ | (英) It has long been believed that tolerance against extreme environments is possible only for 'lower' groups, such as archaea, bacteria or tardigrades, and not for more 'advanced' species. Here, we demonstrated that the mammalian sperm nucleus also exhibited strong tolerance to cold and hot temperatures. When mouse spermatozoa were freeze-dried (FD), similar to the anhydrobiosis of Tardigrades, all spermatozoa were ostensibly dead after rehydration. However, offspring were obtained from recovered FD sperm nuclei, even after repeated treatment with conditions from liquid nitrogen to room temperature. Conversely, when FD spermatozoa were heated at 95 °C, although the birth rate was decreased with increasing duration of the treatment, offspring were obtained even for FD spermatozoa that had been heat-treated for 2 h. This period was improved up to 6 h when glucose was replaced with trehalose in the freeze-drying medium, and the resistance temperature was extended up to 150 °C for short periods of treatment. Randomly selected offspring grew into healthy adults. Our results suggest that, when considering the sperm nucleus/DNA as the material that is used as a blueprint of life, rather than cell viability, a significant tolerance to extreme temperatures is present even in 'higher' species, such as mammals. (日)
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| ○誌名 (必須): | □ | Scientific Reports ([Nature Publishing Group])
(eISSN: 2045-2322)
| ○ISSN (任意): | □ | 2045-2322
ISSN: 2045-2322
(eISSN: 2045-2322) Title: Scientific reportsTitle(ISO): Sci RepPublisher: Nature Portfolio (NLM Catalog)
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| ○巻 (必須): | □ | 9
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| ○号 (必須): | □ | 1
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| ○頁 (必須): | □ | 5719 5719
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| ○年月日 (必須): | □ | 西暦 2019年 4月 5日 (平成 31年 4月 5日)
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| ○DOI (任意): | □ | 10.1038/s41598-019-42062-8 (→Scopusで検索)
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| ○PMID (任意): | □ | 30952922 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1038/s41598-019-42062-8 (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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