『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=354464EID:354464, Map:0, LastModified:2019年8月20日(火) 18:52:52, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[山本 武範], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
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組織 (推奨):
著者 (必須): 1.山本 武範
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2. (英) Ozono Mizune (日) 大園 瑞音 (読) おおぞの みずね
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学籍番号 (推奨): **** [ユーザ]
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3. (英) Watanabe Akira (日) 渡辺 朗 (読) わたなべ あきら
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学籍番号 (推奨): **** [ユーザ]
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4. (英) Maeda Kosuke (日) 前田 康輔 (読) まえだ こうすけ
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学籍番号 (推奨): **** [ユーザ]
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5. (英) Nara Atsushi (日) 奈良 篤 (読) なら あつし
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学籍番号 (推奨): **** [ユーザ]
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6. (英) Hashida Mei (日) 橋田 芽依 (読) はしだ めい
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学籍番号 (推奨): **** [ユーザ]
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7.井戸 佑介
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8.廣島 佑香 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔微生物学])
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9.山田 安希子
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10.寺田 弘 ([東京理科大学])
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11.篠原 康雄 ([徳島大学.先端酵素学研究所.基幹研究部門])
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題名 (必須): (英) Functional analysis of coiled-coil domains of MCU in mitochondrial calcium uptake  (日)    [継承]
副題 (任意):
要約 (任意): (英) The mitochondrial calcium uniporter (MCU) complex is a highly-selective calcium channel. This complex consists of MCU, mitochondrial calcium uptake proteins (MICUs), MCU regulator 1 (MCUR1), essential MCU regulator element (EMRE), etc. MCU, which is the pore-forming subunit, has 2 highly conserved coiled-coil domains (CC1 and CC2); however, their functional roles are unknown. The yeast expression system of mammalian MCU and EMRE enables precise reconstitution of the properties of the mammalian MCU complex in yeast mitochondria. Using the yeast expression system, we here showed that, when MCU mutant lacking CC1 or CC2 was expressed together with EMRE in yeast, their mitochondrial Ca-uptake function was lost. Additionally, point mutations in CC1 or CC2, which were expected to prevent the formation of the coiled coil, also disrupted the Ca-uptake function. Thus, it is essential for the Ca uptake function of MCU that the coiled-coil structure be formed in CC1 and CC2. The loss of function of those mutated MCUs was also observed in the mitochondria of a yeast strain lacking the yeast MCUR1 homolog. Also, in the D. discoideum MCU, which has EMRE-independent Ca-uptake function, the deletion of either CC1 or CC2 caused the loss of function. These results indicated that the critical functions of CC1 and CC2 were independent of other regulatory subunits such as MCUR1 and EMRE, suggesting that CC1 and CC2 might be essential for pore formation by MCUs themselves. Based on the tetrameric structure of MCU, we discussed the functional roles of the coiled-coil domains of MCU.  (日)    [継承]
キーワード (推奨): 1.カルシウム (calcium) [継承]
2.イオンチャネル (ion channel) [継承]
3.ミトコンドリア (mitochondria) [継承]
4.コイルドコイルモチーフ (coiled-coil motif) [継承]
5. (英) yeast (日) 酵母 (読) こうぼ [継承]
発行所 (推奨):
誌名 (必須): Biochimica et Biophysica Acta (BBA) - Bioenergetics ([Elsevier Science])
(pISSN: 0005-2728, eISSN: 1879-2650)

ISSN (任意): 1879-2650
ISSN: 0005-2728 (pISSN: 0005-2728, eISSN: 1879-2650)
Title: Biochimica et biophysica acta. Bioenergetics
Title(ISO): Biochim Biophys Acta Bioenerg
Publisher: Elsevier BV
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 148061 148061 [継承]
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年月日 (必須): 西暦 2019年 8月 3日 (令和 元年 8月 3日) [継承]
URL (任意):
DOI (任意): 10.1016/j.bbabio.2019.148061    (→Scopusで検索) [継承]
PMID (任意): 31394096    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) Article.ELocationID: S0005-2728(19)30100-8  (日)    [継承]
2.(英) Article.ELocationID: 10.1016/j.bbabio.2019.148061  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
4.(英) KeywordList.Keyword: Calcium uniporter  (日)    [継承]
5.(英) KeywordList.Keyword: Coiled coil  (日)    [継承]
6.(英) KeywordList.Keyword: Ion channel  (日)    [継承]
7.(英) KeywordList.Keyword: MCU  (日)    [継承]
8.(英) KeywordList.Keyword: Mitochondria  (日)    [継承]
9.(英) KeywordList.Keyword: Yeast  (日)    [継承]

標準的な表示

和文冊子 ● Takenori Yamamoto, Mizune Ozono, Akira Watanabe, Kosuke Maeda, Atsushi Nara, Mei Hashida, Yusuke Ido, Yuka Hiroshima, Akiko Yamada, Hiroshi Terada and Yasuo Shinohara : Functional analysis of coiled-coil domains of MCU in mitochondrial calcium uptake, Biochimica et Biophysica Acta (BBA) - Bioenergetics, 148061, 2019.
欧文冊子 ● Takenori Yamamoto, Mizune Ozono, Akira Watanabe, Kosuke Maeda, Atsushi Nara, Mei Hashida, Yusuke Ido, Yuka Hiroshima, Akiko Yamada, Hiroshi Terada and Yasuo Shinohara : Functional analysis of coiled-coil domains of MCU in mitochondrial calcium uptake, Biochimica et Biophysica Acta (BBA) - Bioenergetics, 148061, 2019.

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