『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (紀要その他) [継承]
言語 (必須): 日本語 [継承]
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著者 (必須): 1. (英) Kawano Tomoharu (日) 河野 友晴 (読)
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2.松久 宗英 ([徳島大学.先端酵素学研究所.基幹研究部門]/徳島大学病院アンチエイジングセンター センター長(併任))
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3.橋田 誠一 ([宮崎医科大学])
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[継承]
4. (英) Numata Satoshi (日) 沼田 聡 (読)
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5.藤本 一幸
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6.黒田 暁生 ([徳島大学.先端酵素学研究所.基幹研究部門])
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7. (英) Yasuda Tetsuyuki (日) 安田 哲行 (読)
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8. (英) Miyashita Kazuyuki (日) 宮下 和幸 (読)
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9. (英) Sakamoto Fumie (日) 坂本 扶美枝 (読)
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10. (英) Katakami Naoto (日) 片上 直人 (読)
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11. (英) Matsuoka Taka-aki (日) 松岡 孝昭 (読)
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題名 (必須): (英) Development of highly sensitive enzyme immunoassay (ICT-EIA) for IA-2 autoantibody and application for detection of three islet-specific autoantibodies ( GADA, IA-2A and IAA) in type I diabetes mellitus  (日) IA-2抗体の高感度検出法(ICT-EIA法)の開発と長期罹患若年性1型糖尿病患者における3種膵島関連自己抗体(GADA,IA-2A,IAA)の検出について   [継承]
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要約 (任意): (英) <p>Development of highly sensitive enzyme immunoassay (ICT-EIA) for IA-2 autoantibody and application for detection of three islet-specific autoantibodies ( GADA, IA-2A and IAA) in type I diabetes mellitus Tomoharu Kawano, Satoshi Numata, Yuki Fujimoto, Akio Kuroda, Tetsuyuki Yasuda,Kazuyuki Miyashita, Fumie Sakamoto, Naoto Katakami, Taka-aki Matsuoka, Munehide Matsuhisa and Seiichi Hashida Summary Detection of pancreatic islet-related autoantibodies( insulin antibody [IAA]; glutamic acid decarboxylase antibody [GADA]; and islet antigen-2 antibody [IA-2A], is essential for the diagnosis and prediction of the onset of type I diabetes mellitus. Depending on the disease duration, the detection rate of each autoantibody is different, and using only one kind of autoantibody detection risks overlooking a positive result. The detection precision of type I diabetes mellitus would thus be improved by measuring all three kinds of autoantibodies. We developed a highly sensitive enzyme immunoassay( ICT-EIA) for IA-2 autoantibody. ICT-EIAs for IAA and GADA have already been developed. Using three ICT-EIAs, we attempted to measure autoantibodies in sera from patients with type I diabetes mellitus. Patients comprised 25 males and 51 females( mean age, 33.3±5.7 years; disease duration, 23.9±6.7 years) with type I diabetes mellitus who had been undergoing treatment with insulin for between 11 and 40 years. Among these patients, urinary C-peptide-positive results were seen in three patients. IAA, GADA and IA-2A in sera were measured by ICT-EIA. The newly developed ICT-EIA for IA-2 antibodies was approximately 100-times more sensitive than the conventional ELISA. The positive ratio was 77.6%, representing a high rate of detection. In addition, even in patients with a disease duration >30 years, the positive rate was 64.3%. ICT-EIA was thus suggested to be useful in combination assays for pancreatic islet-related autoantibodies (IAA, GADA and IA-2A).</p><p> </p><p></p>  (日) <p>Development of highly sensitive enzyme immunoassay (ICT-EIA) for IA-2 autoantibody and application for detection of three islet-specific autoantibodies ( GADA, IA-2A and IAA) in type I diabetes mellitus Tomoharu Kawano, Satoshi Numata, Yuki Fujimoto, Akio Kuroda, Tetsuyuki Yasuda,Kazuyuki Miyashita, Fumie Sakamoto, Naoto Katakami, Taka-aki Matsuoka, Munehide Matsuhisa and Seiichi Hashida Summary Detection of pancreatic islet-related autoantibodies( insulin antibody [IAA]; glutamic acid decarboxylase antibody [GADA]; and islet antigen-2 antibody [IA-2A], is essential for the diagnosis and prediction of the onset of type I diabetes mellitus. Depending on the disease duration, the detection rate of each autoantibody is different, and using only one kind of autoantibody detection risks overlooking a positive result. The detection precision of type I diabetes mellitus would thus be improved by measuring all three kinds of autoantibodies. We developed a highly sensitive enzyme immunoassay( ICT-EIA) for IA-2 autoantibody. ICT-EIAs for IAA and GADA have already been developed. Using three ICT-EIAs, we attempted to measure autoantibodies in sera from patients with type I diabetes mellitus. Patients comprised 25 males and 51 females( mean age, 33.3±5.7 years; disease duration, 23.9±6.7 years) with type I diabetes mellitus who had been undergoing treatment with insulin for between 11 and 40 years. Among these patients, urinary C-peptide-positive results were seen in three patients. IAA, GADA and IA-2A in sera were measured by ICT-EIA. The newly developed ICT-EIA for IA-2 antibodies was approximately 100-times more sensitive than the conventional ELISA. The positive ratio was 77.6%, representing a high rate of detection. In addition, even in patients with a disease duration >30 years, the positive rate was 64.3%. ICT-EIA was thus suggested to be useful in combination assays for pancreatic islet-related autoantibodies (IAA, GADA and IA-2A).</p><p> </p><p></p>   [継承]
キーワード (推奨):
発行所 (推奨): 徳島文理大学 [継承]
誌名 (必須): 徳島文理大学研究紀要 ([徳島文理大学])
(pISSN: 0286-9829)

ISSN (任意): 0286-9829
ISSN: 0286-9829 (pISSN: 0286-9829)
Title: 徳島文理大学研究紀要
Supplier: 徳島文理大学
 (Webcat Plus  (医中誌Web (No Scopus information.)
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(必須): 96 [継承]
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(必須): 35 44 [継承]
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年月日 (必須): 西暦 2018年 9月 初日 (平成 30年 9月 初日) [継承]
URL (任意): https://ci.nii.ac.jp/naid/130007602165/ [継承]
DOI (任意): 10.24596/tokusimabunriu.96.0_35    (→Scopusで検索) [継承]
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NAID (任意): 130007602165 [継承]
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和文冊子 ● 河野 友晴, 松久 宗英, 橋田 誠一, 沼田 聡, 藤本 一幸, 黒田 暁生, 安田 哲行, 宮下 和幸, 坂本 扶美枝, 片上 直人, 松岡 孝昭 : IA-2抗体の高感度検出法(ICT-EIA法)の開発と長期罹患若年性1型糖尿病患者における3種膵島関連自己抗体(GADA,IA-2A,IAA)の検出について, 徳島文理大学研究紀要, Vol.96, 35-44, 2018年.
欧文冊子 ● Tomoharu Kawano, Munehide Matsuhisa, Seiichi Hashida, Satoshi Numata, Kazuyuki Fujimoto, Akio Kuroda, Tetsuyuki Yasuda, Kazuyuki Miyashita, Fumie Sakamoto, Naoto Katakami and Taka-aki Matsuoka : Development of highly sensitive enzyme immunoassay (ICT-EIA) for IA-2 autoantibody and application for detection of three islet-specific autoantibodies ( GADA, IA-2A and IAA) in type I diabetes mellitus, Research bulletin of Tokushima Bunri University, Vol.96, 35-44, 2018.

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