『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=350850EID:350850, Map:0, LastModified:2019年8月19日(月) 17:30:49, Operator:[鬼塚 正義], Avail:TRUE, Censor:0, Owner:[鬼塚 正義], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨): 国内共著 (徳島大学内研究者と国内(学外)研究者との共同研究 (国外研究者を含まない)) [継承]
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.鬼塚 正義 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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2. (英) Kadoya Yukinori (日) ⻆屋 行紀 (読) かどや ゆきのり
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学籍番号 (推奨): **** [ユーザ]
[継承]
3.大政 健史
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学籍番号 (推奨):
[継承]
題名 (必須): (英) Secretory leakage of IgG1 aggregates from recombinant Chinese hamster ovary cells  (日)    [継承]
副題 (任意):
要約 (任意): (英) Aggregation of therapeutic antibodies is one of the most important issues to be resolved in manufacturing processes because of reduced efficacy and immunogenicity. Despite aggregation studies in vitro, little is known about the aggregation mechanism in cell culture processes. In this study, we investigated the process of aggregate formation of IgG1 antibodies during the culture of Chinese hamster ovary (CHO) cells to determine how aggregation occurs. A recombinant CHO cell line was cultivated in a bioreactor, and purified IgG1 from daily culture supernatants was analyzed by size exclusion chromatography. We found a linear correlation between the peak plots of IgG1 by-products, dimeric and aggregated IgG1, and integrated viable cell density, indicating that these by-products were secreted from CHO cells at a constant secretion rate. In addition, aggregate formation was not reproduced in pseudo-culture experiments, and the solution structures of intracellular and extracellular IgG1 aggregates were similar. These results support the concept of secretory leakage of IgG1 by-products. Secreted aggregates appeared to be in an alternatively folded state, which can pass through the protein quality control system in mammalian cells.  (日)    [継承]
キーワード (推奨):
発行所 (推奨):
誌名 (必須): Journal of Bioscience and Bioengineering ([日本生物工学会])
(pISSN: 1389-1723, eISSN: 1347-4421)

ISSN (任意): 1347-4421
ISSN: 1389-1723 (pISSN: 1389-1723, eISSN: 1347-4421)
Title: Journal of bioscience and bioengineering
Title(ISO): J Biosci Bioeng
Supplier: 公益社団法人日本生物工学会
Publisher: Elsevier BV
 (NLM Catalog  (Webcat Plus  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 127 [継承]
(必須): 6 [継承]
(必須): 752 757 [継承]
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年月日 (必須): 西暦 2019年 6月 初日 (令和 元年 6月 初日) [継承]
URL (任意):
DOI (任意): 10.1016/j.jbiosc.2018.11.015    (→Scopusで検索) [継承]
PMID (任意): 30580968    (→Scopusで検索) [継承]
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Scopus (任意): 2-s2.0-85058714330 [継承]
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備考 (任意): 1.(英) Article.ELocationID: S1389-1723(18)30828-4  (日)    [継承]
2.(英) Article.ELocationID: 10.1016/j.jbiosc.2018.11.015  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
4.(英) KeywordList.Keyword: Antibody aggregation  (日)    [継承]
5.(英) KeywordList.Keyword: Antibody production  (日)    [継承]
6.(英) KeywordList.Keyword: Chinese hamster ovary cells  (日)    [継承]
7.(英) KeywordList.Keyword: Monoclonal antibody  (日)    [継承]
8.(英) KeywordList.Keyword: Protein quality control  (日)    [継承]
9.(英) KeywordList.Keyword: Protein secretion  (日)    [継承]

標準的な表示

和文冊子 ● Masayoshi Onitsuka, Yukinori Kadoya and Takeshi Omasa : Secretory leakage of IgG1 aggregates from recombinant Chinese hamster ovary cells, Journal of Bioscience and Bioengineering, Vol.127, No.6, 752-757, 2019.
欧文冊子 ● Masayoshi Onitsuka, Yukinori Kadoya and Takeshi Omasa : Secretory leakage of IgG1 aggregates from recombinant Chinese hamster ovary cells, Journal of Bioscience and Bioengineering, Vol.127, No.6, 752-757, 2019.

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