『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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審査 (推奨): Peer Review [継承]
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共著種別 (推奨): 国内共著 (徳島大学内研究者と国内(学外)研究者との共同研究 (国外研究者を含まない)) [継承]
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Kaneyoshi Kohei (日) (読)
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2. (英) Kuroda Kouki (日) (読)
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3.内山 圭司 ([徳島大学.先端酵素学研究所.基幹研究部門])
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4.鬼塚 正義 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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5. (英) Yamano-Adachi Noriko (日) (読)
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6. (英) Koga Yuichi (日) (読)
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7.大政 健史
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題名 (必須): (英) Secretion analysis of intracellular difficult-to-express immunoglobulin G (IgG) in Chinese hamster ovary (CHO) cells  (日)    [継承]
副題 (任意):
要約 (任意): (英) The Chinese hamster ovary (CHO) cell line is the most widely used host cell for therapeutic antibody production. Although its productivity has been improved by various strategies to satisfy the growing global demand, some difficult-to-express (DTE) antibodies remain at low secretion levels. To improve the production of various therapeutic antibodies, it is necessary to determine possible rate-limiting steps in DTE antibody secretion in comparison with other high IgG producers. Here, we analyzed the protein secretion process in CHO cells producing the DTE immunoglobulin G (IgG) infliximab. The results from chase assays using a translation inhibitor revealed that infliximab secretion could be nearly completed within 2 h, at which time the cells still retained about 40% of heavy chains and 65% of light chains. Using fluorescent microscopy, we observed that these IgG chains remained in the endoplasmic reticulum and Golgi apparatus. The cells inefficiently form fully assembled heterodimer IgG by making LC aggregates, which may be the most serious bottleneck in the production of DTE infliximab compared with other IgG high producers. Our study could contribute to establish the common strategy for constructing DTE high-producer cells on the basis of rate-limiting step analysis.  (日)    [継承]
キーワード (推奨):
発行所 (推奨):
誌名 (必須): Cytotechnology (Springer Netherlands)
(pISSN: 0920-9069, eISSN: 1573-0778)

ISSN (任意): 0920-9069
ISSN: 0920-9069 (pISSN: 0920-9069, eISSN: 1573-0778)
Title: Cytotechnology
Title(ISO): Cytotechnology
Supplier: Kluwer Online
Publisher: Springer Netherlands
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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年月日 (必須): 西暦 2019年 2月 初日 (平成 31年 2月 初日) [継承]
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DOI (任意): 10.1007/s10616-018-0286-5    (→Scopusで検索) [継承]
PMID (任意): 30637508    (→Scopusで検索) [継承]
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Scopus (任意): 2-s2.0-85060048909 [継承]
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備考 (任意): 1.(英) Article.ELocationID: 10.1007/s10616-018-0286-5  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) KeywordList.Keyword: Animal cell culture  (日)    [継承]
4.(英) KeywordList.Keyword: Chinese hamster ovary cell  (日)    [継承]
5.(英) KeywordList.Keyword: Difficult-to-express IgG  (日)    [継承]
6.(英) KeywordList.Keyword: Protein secretion  (日)    [継承]
7.(英) KeywordList.Keyword: Therapeutic antibody production  (日)    [継承]

標準的な表示

和文冊子 ● Kohei Kaneyoshi, Kouki Kuroda, Keiji Uchiyama, Masayoshi Onitsuka, Noriko Yamano-Adachi, Yuichi Koga and Takeshi Omasa : Secretion analysis of intracellular difficult-to-express immunoglobulin G (IgG) in Chinese hamster ovary (CHO) cells, Cytotechnology, Vol.71, No.1, 305-316, 2019.
欧文冊子 ● Kohei Kaneyoshi, Kouki Kuroda, Keiji Uchiyama, Masayoshi Onitsuka, Noriko Yamano-Adachi, Yuichi Koga and Takeshi Omasa : Secretion analysis of intracellular difficult-to-express immunoglobulin G (IgG) in Chinese hamster ovary (CHO) cells, Cytotechnology, Vol.71, No.1, 305-316, 2019.

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