『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=342965EID:342965, Map:0, LastModified:2019年8月13日(火) 02:52:07, Operator:[内山 圭司], Avail:TRUE, Censor:0, Owner:[内山 圭司], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Kaneyoshi Kohei (日) (読)
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2.内山 圭司 ([徳島大学.先端酵素学研究所.基幹研究部門])
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3.鬼塚 正義 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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4.山野 範子
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5. (英) Koga Yuichi (日) (読)
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6.大政 健史
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題名 (必須): (英) Analysis of intracellular IgG secretion in Chinese hamster ovary cells to improve IgG production.  (日)    [継承]
副題 (任意):
要約 (任意): (英) The production of biopharmaceutical immunoglobulin G (IgG) using cultured mammalian cells, especially Chinese hamster ovary (CHO) cells is well established and has been markedly improved through the modification of cells and cell culture engineering technologies. The establishment of high-production cell lines remains a challenge. The intracellular secretion of IgG has been investigated to identify and solve the rate-limiting steps in antibody production. However, strategies that regulate the expression of proteins that are related to antibody secretory pathway have not consistently improved their production. In this study, key features and limitations of the antibody secretion process in recombinant CHO cells were analyzed to develop more efficient approaches for establishing high-production cells. By chase assay with protein translation inhibitors, IgG secretion reached a plateau when at least 20% of IgG remained in the cells. The secretion kinetics and retention ratio of IgG varied between IgG subclasses (two types of IgG1 and an IgG3 subclass). Immunofluorescent microscopy and size exclusion chromatography showed that the remaining intracellular IgG localized mainly within the endoplasmic reticulum (ER) and less with the cis-Golgi network, despite the formation of fully assembled IgG. These results show that remaining intracellular IgG is a target for enhancing antibody secretion, even in high-production CHO cells.  (日)    [継承]
キーワード (推奨):
発行所 (推奨):
誌名 (必須): Journal of Bioscience and Bioengineering ([日本生物工学会])
(pISSN: 1389-1723, eISSN: 1347-4421)

ISSN (任意): 1347-4421
ISSN: 1389-1723 (pISSN: 1389-1723, eISSN: 1347-4421)
Title: Journal of bioscience and bioengineering
Title(ISO): J Biosci Bioeng
Supplier: 公益社団法人日本生物工学会
Publisher: Elsevier BV
 (NLM Catalog  (Webcat Plus  (医中誌Web  (J-STAGE  (Scopus  (CrossRef (Scopus information is found. [need login])
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年月日 (必須): 西暦 2019年 1月 末日 (平成 31年 1月 末日) [継承]
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DOI (任意): 10.1016/j.jbiosc.2018.06.018    (→Scopusで検索) [継承]
PMID (任意): 30017708    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Kohei Kaneyoshi, Keiji Uchiyama, Masayoshi Onitsuka, Noriko Yamano, Yuichi Koga and Takeshi Omasa : Analysis of intracellular IgG secretion in Chinese hamster ovary cells to improve IgG production., Journal of Bioscience and Bioengineering, Vol.127, No.1, 107-113, 2019.
欧文冊子 ● Kohei Kaneyoshi, Keiji Uchiyama, Masayoshi Onitsuka, Noriko Yamano, Yuichi Koga and Takeshi Omasa : Analysis of intracellular IgG secretion in Chinese hamster ovary cells to improve IgG production., Journal of Bioscience and Bioengineering, Vol.127, No.1, 107-113, 2019.

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