『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=342279EID:342279, Map:0, LastModified:2019年1月21日(月) 17:11:52, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[川上 竜巳], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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審査 (推奨): Peer Review [継承]
カテゴリ (推奨): 研究 [継承]
共著種別 (推奨): 国内共著 (徳島大学内研究者と国内(学外)研究者との共同研究 (国外研究者を含まない)) [継承]
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著者 (必須): 1.川上 竜巳 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.食料科学系.食料科学分野]/[徳島大学.生物資源産業学部])
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2. (英) Ohshida Tatsuya (日) 大志田 達也 (読)
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3.櫻庭 春彦
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4.大島 敏久 ([九州大学])
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題名 (必須): (英) A novel PLP-dependent Alanine/Serine racemase from the hyperthermophilic archaeon Pyrococcus horikoshii OT-3  (日)    [継承]
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要約 (任意): (英) We recently identified and characterized a novel broad substrate specificity amino acidracemase (BAR) from the hyperthermophilic archaeon Pyrococcus horikoshii OT-3.Three genes, PH0782, PH1423, and PH1501, encoding homologs exhibiting about45% sequence identity with BAR were present in the P. horikoshii genome. In this study,we detected pyridoxal 50-phosphate (PLP)-dependent amino acid racemase activity inthe protein encoded by PH0782. The enzyme showed activity toward Ala, Ser, Thr,and Val, but the catalytic efficiency with Thr or Val was much lower than with Ala orSer. The enzyme was therefore designated Ala/Ser racemase (ASR). Like BAR, ASRwas highly stable at high temperatures and over a wide range of pHs, though itshexameric structure differed from the dimeric structure of BAR. No activity was detectedin K291A or D234A ASR mutants. This suggests that, as in Ile 2-epimerase (ILEP) fromLactobacillus buchneri JCM1115, these residues are involved in Schiff base formationand substrate interaction, respectively. Unlike BAR, enhanced ASR activity was notdetected in P. horikoshii cells cultivated in the presence of D-Ala or D-Ser. This is thefirst description of a PLP-dependent fold type I ASR in archaea.  (日)    [継承]
キーワード (推奨): 1. (英) Ala racemase (日) (読) [継承]
2. (英) Ser racemase (日) (読) [継承]
3. (英) PLP-dependent enzyme (日) (読) [継承]
4. (英) Pyrococcus horikoshii (日) (読) [継承]
5. (英) hyperthermophilic archaea (日) (読) [継承]
6. (英) D-amino acid (日) (読) [継承]
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誌名 (必須): Frontiers in Microbiology (Frontiers Media S.A.)
(eISSN: 1664-302X)

ISSN (任意): 1664-302X
ISSN: 1664-302X (eISSN: 1664-302X)
Title: Frontiers in microbiology
Title(ISO): Front Microbiol
Publisher: Frontiers Media S.A.
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年月日 (必須): 西暦 2018年 7月 9日 (平成 30年 7月 9日) [継承]
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DOI (任意): 10.3389/fmicb.2018.01481    (→Scopusで検索) [継承]
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Scopus (任意): 2-s2.0-85049861690 [継承]
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和文冊子 ● Ryushi Kawakami, Tatsuya Ohshida, Haruhiko Sakuraba and Toshihisa Ohshima : A novel PLP-dependent Alanine/Serine racemase from the hyperthermophilic archaeon Pyrococcus horikoshii OT-3, Frontiers in Microbiology, 2018.
欧文冊子 ● Ryushi Kawakami, Tatsuya Ohshida, Haruhiko Sakuraba and Toshihisa Ohshima : A novel PLP-dependent Alanine/Serine racemase from the hyperthermophilic archaeon Pyrococcus horikoshii OT-3, Frontiers in Microbiology, 2018.

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