『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=337040EID:337040, Map:0, LastModified:2018年12月28日(金) 16:58:12, Operator:[三木 ちひろ], Avail:TRUE, Censor:0, Owner:[酒井 仁美], Read:継承, Write:継承, Delete:継承.
○種別 (必須): □学術論文 (審査論文) [継承]
○言語 (必須): □英語 [継承]
○招待 (推奨):
○審査 (推奨):
○カテゴリ (推奨):
○共著種別 (推奨):
○学究種別 (推奨):
○組織 (推奨):
○著者 (必須): 1. (英) Hatakeyama Tomomitsu (日) (読)
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○学籍番号 (推奨):
[継承]
2. (英) Ichise Ayaka (日) (読)
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3. (英) Unno Hideaki (日) (読)
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[継承]
4.郷田 秀一郎 ([長崎大学])
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5. (英) Oda Tatsuya (日) (読)
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6. (英) Tateno Hiroaki (日) (読)
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7. (英) Hirabayashi Jun (日) (読)
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8.酒井 仁美 ([徳島大学.技術支援部.常三島技術部門.地域協働グループ])
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○学籍番号 (推奨): □**** [ユーザ]
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9.中川 秀幸
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○題名 (必須): □(英) Carbohydrate recognition by the rhamnose-binding lectin SUL-I with a novel three-domain structure isolated from the venom of globiferous pedicellariae of the flower sea urchin Toxopneustes pileolus  (日)    [継承]
○副題 (任意):
○要約 (任意): □(英) The globiferous pedicellariae of the venomous sea urchin Toxopneustes pileolus contains several biologically active proteins. We have cloned the cDNA of one of the toxin components, SUL-I, which is a rhamnose-binding lectin (RBL) that acts as a mitogen through binding to carbohydrate chains on target cells. Recombinant SUL-I (rSUL-I) was produced in Escherichia coli cells, and its carbohydrate-binding specificity was examined with the glycoconjugate microarray analysis, which suggested that potential target carbohydrate structures are galactose-terminated N-glycans. rSUL-I exhibited mitogenic activity for murine splenocyte cells and toxicity against Vero cells. The three-dimensional structure of the rSUL-I/l-rhamnose complex was determined by X-ray crystallographic analysis at a 1.8 Å resolution. The overall structure of rSUL-I is composed of three distinctive domains with a folding structure similar to those of CSL3, a RBL from chum salmon (Oncorhynchus keta) eggs. The bound l-rhamnose molecules are mainly recognized by rSUL-I through hydrogen bonds between its 2-, 3-, and 4-hydroxy groups and Asp, Asn, and Glu residues in the binding sites, while Tyr and Ser residues participate in the recognition mechanism. It was also inferred that SUL-I may form a dimer in solution based on the molecular size estimated via dynamic light scattering as well as possible contact regions in its crystal structure.  (日)    [継承]
○キーワード (推奨): 1. (英) Amino Acid Sequence (日) (読) [継承]
2. (英) Animal Structures (日) (読) [継承]
3. (英) Animals (日) (読) [継承]
4. (英) Binding Sites (日) (読) [継承]
5. (英) Carbohydrate Sequence (日) (読) [継承]
6. (英) Cercopithecus aethiops (日) (読) [継承]
7. (英) Cloning, Molecular (日) (読) [継承]
8.X線結晶学 (X-ray crystallography) [継承]
9.大腸菌 (Escherichia coli) [継承]
10.遺伝子発現 (gene expression) [継承]
11. (英) Hydrogen Bonding (日) (読) [継承]
12. (英) Lectins (日) (読) [継承]
13. (英) Lymphocytes (日) (読) [継承]
14. (英) Marine Toxins (日) (読) [継承]
15. (英) Mice (日) (読) [継承]
16. (英) Microarray Analysis (日) (読) [継承]
17. (英) Mitogens (日) (読) [継承]
18. (英) Models, Molecular (日) (読) [継承]
19. (英) Polysaccharides (日) (読) [継承]
20. (英) Protein Binding (日) (読) [継承]
21. (英) Protein Interaction Domains and Motifs (日) (読) [継承]
22. (英) Protein Multimerization (日) (読) [継承]
23. (英) Protein Structure, Secondary (日) (読) [継承]
24. (英) Recombinant Proteins (日) (読) [継承]
25. (英) Rhamnose (日) (読) [継承]
26. (英) Sea Urchins (日) (読) [継承]
27. (英) Vero Cells (日) (読) [継承]
○発行所 (推奨):
○誌名 (必須): □Protein Science (Protein Society)
(pISSN: 0961-8368, eISSN: 1469-896X)

○ISSN (任意): □1469-896X
ISSN: 0961-8368 (pISSN: 0961-8368, eISSN: 1469-896X)
Title: Protein science : a publication of the Protein Society
Title(ISO): Protein Sci
Supplier: The Protein Society
Publisher: Cambridge University Press
 (NLM Catalog)   (Wiley)   (Scopus)   (CrossRef)  (Scopus information is found. [need login])
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○巻 (必須): □276 [継承]
○号 (必須): □8 [継承]
○頁 (必須): □1574 1583 [継承]
○都市 (任意):
○年月日 (必須): □西暦 2017年 5月 12日 (平成 29年 5月 12日) [継承]
○URL (任意):
○DOI (任意): □10.1002/pro.3185    (→Scopusで検索) [継承]
○PMID (任意): □28470711    (→Scopusで検索) [継承]
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○Scopus (任意): □2-s2.0-85018917301 [継承]
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○備考 (任意): 1.(英) Article.ELocationID: 10.1002/pro.3185  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) KeywordList.Keyword: X-ray crystallographic analysis  (日)    [継承]
4.(英) KeywordList.Keyword: lectin  (日)    [継承]
5.(英) KeywordList.Keyword: rhamnose  (日)    [継承]
6.(英) KeywordList.Keyword: sea urchin  (日)    [継承]
7.(英) KeywordList.Keyword: toxin  (日)    [継承]

標準的な表示

和文冊子 ● Tomomitsu Hatakeyama, Ayaka Ichise, Hideaki Unno, Shuichiro Goda, Tatsuya Oda, Hiroaki Tateno, Jun Hirabayashi, Hitomi Sakai and Hideyuki Nakagawa : Carbohydrate recognition by the rhamnose-binding lectin SUL-I with a novel three-domain structure isolated from the venom of globiferous pedicellariae of the flower sea urchin Toxopneustes pileolus, Protein Science, Vol.276, No.8, 1574-1583, 2017.
欧文冊子 ● Tomomitsu Hatakeyama, Ayaka Ichise, Hideaki Unno, Shuichiro Goda, Tatsuya Oda, Hiroaki Tateno, Jun Hirabayashi, Hitomi Sakai and Hideyuki Nakagawa : Carbohydrate recognition by the rhamnose-binding lectin SUL-I with a novel three-domain structure isolated from the venom of globiferous pedicellariae of the flower sea urchin Toxopneustes pileolus, Protein Science, Vol.276, No.8, 1574-1583, 2017.

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