『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=336852EID:336852, Map:0, LastModified:2019年3月2日(土) 20:40:50, Operator:[[ADMIN]], Avail:TRUE, Censor:0, Owner:[野間 隆文], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
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著者 (必須): 1. (英) Arinawati Dian Yosi (日) Dian Yosi Arinawati (読) でぃあん よし ありなわてぃ
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学籍番号 (推奨): **** [ユーザ]
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2.三好 圭子 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.分子医化学])
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3.谷村 綾子
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4.堀口 大吾 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.分子医化学])
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5. (英) Hagita Hiroko (日) (読)
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6.野間 隆文 ([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.分子医化学])
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題名 (必須): (英) Deciphering defective amelogenesis using invitro culture systems.  (日)    [継承]
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要約 (任意): (英)   (日) The conventional two-dimensional (2D) in vitro culture system is frequently used to analyze the gene expression with or without extracellular signals. However, the cells derived from primary culture and cell lines frequently deviate the gene expression profile compared to the corresponding in vivo samples, which sometimes misleads the actual gene regulation in vivo. To overcome this gap, we developed the comparative 2D and 3D in vitro culture systems and applied them to the genetic study of amelogenesis imperfecta (AI) as a model. Recently, we found specificity protein 6 (Sp6) mutation in an autosomal-recessive AI rat that was previously named AMI. We constructed 3D structure of ARE-B30 cells (AMI-derived rat dental epithelial cells) or G5 (control wild type cells) combined with RPC-C2A cells (rat pulp cell line) separated by the collagen membrane, while in 2D structure, ARE-B30 or G5 was cultured with or without the collagen membrane. Comparative analysis of amelogenesis-related gene expression in ARE-B30 and G5 using our 2D and 3D in vitro systems revealed distinct expression profiles, showing the causative outcomes. Bone morphogenetic protein 2 and follistatin were reciprocally expressed in G5, but not in ARE-B30 cells. All-or-none expression of amelotin, kallikrein-related peptidase 4, and nerve growth factor receptor was observed in both cell types. In conclusion, our in vitro culture systems detected the phenotypical differences in the expression of the stage-specific amelogenesis-related genes. Parallel analysis with 2D and 3D culture systems may provide a platform to understand the molecular basis for defective amelogenesis caused by Sp6 mutation.   [継承]
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誌名 (必須): Journal of Bioscience and Bioengineering ([日本生物工学会])
(pISSN: 1389-1723, eISSN: 1347-4421)

ISSN (任意): 1347-4421
ISSN: 1389-1723 (pISSN: 1389-1723, eISSN: 1347-4421)
Title: Journal of bioscience and bioengineering
Title(ISO): J. Biosci. Bioeng.
Supplier: 公益社団法人日本生物工学会
Publisher: Elsevier BV
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(必須): Volume 125 [継承]
(必須): Issue 4 [継承]
(必須): 365 496 [継承]
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年月日 (必須): 西暦 2018年 4月 1日 (平成 30年 4月 1日) [継承]
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DOI (任意): 10.1016/j.jbiosc.2017.11.009    (→Scopusで検索) [継承]
PMID (任意): 29397320    (→Scopusで検索) [継承]
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機関リポジトリ : 112952 [継承]
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備考 (任意): 1.(英) PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Yosi Dian Arinawati, Keiko Miyoshi, Ayako Tanimura, Taigo Horiguchi, Hiroko Hagita and Takafumi Noma : Deciphering defective amelogenesis using invitro culture systems., Journal of Bioscience and Bioengineering, Vol.Volume 125, No.Issue 4, 365-496, 2018.
欧文冊子 ● Yosi Dian Arinawati, Keiko Miyoshi, Ayako Tanimura, Taigo Horiguchi, Hiroko Hagita and Takafumi Noma : Deciphering defective amelogenesis using invitro culture systems., Journal of Bioscience and Bioengineering, Vol.Volume 125, No.Issue 4, 365-496, 2018.

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