『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=332649)

EID=332649EID:332649, Map:0, LastModified:2017年8月29日(火) 09:27:54, Operator:[佐藤 康史], Avail:TRUE, Censor:0, Owner:[佐藤 康史], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Sato Tsutomu (日) (読)
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[継承]
2. (英) Machida Takuro (日) (読)
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[継承]
3. (英) Takahashi Sho (日) (読)
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[継承]
4. (英) Iyama Satoshi (日) (読)
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[継承]
5.佐藤 康史 ([徳島大学.大学院医歯薬学研究部.医学域.連携研究部門(医学域).寄附講座系(医学域).地域消化器・総合内科学])
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6. (英) Kuribayashi Kageaki (日) (読)
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[継承]
7. (英) Takada Kohichi (日) (読)
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8. (英) Oku Takatomi (日) (読)
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9. (英) Kawano Yutaka (日) (読)
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10. (英) Okamoto Tetsuro (日) (読)
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11. (英) Takimoto Rishu (日) (読)
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12. (英) Matsunaga Takuya (日) (読)
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13.高山 哲治 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.内科系.消化器内科学])
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14. (英) Takahashi Minoru (日) (読)
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15. (英) Kato Junji (日) (読)
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16. (英) Niitsu Yoshiro (日) (読)
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題名 (必須): (英) Fas-mediated apoptosome formation is dependent on reactive oxygen species derived from mitochondrial permeability transition in Jurkat cells.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Generation of reactive oxygen species (ROS) and activation of caspase cascade are both indispensable in Fas-mediated apoptotic signaling. Although ROS was presumed to affect the activity of the caspase cascade on the basis of findings that antioxidants inhibited the activation of caspases and that the stimulation of ROS by itself activated caspases, the mechanism by which these cellular events are integrated in Fas signaling is presently unclear. In this study, using human T cell leukemia Jurkat cells as well as an in vitro reconstitution system, we demonstrate that ROS are required for the formation of apoptosome. We first showed that ROS derived from mitochondrial permeability transition positively regulated the apoptotic events downstream of mitochondrial permeability transition. Then, we revealed that apoptosome formation in Fas-stimulated Jurkat cells was clearly inhibited by N-acetyl-L-cysteine and manganese superoxide dismutase by using both the immunoprecipitation and size-exclusion chromatography methods. To confirm these in vivo findings, we next used an in vitro reconstitution system in which in vitro-translated apoptotic protease-activating factor 1 (Apaf-1), procaspase-9, and cytochrome c purified from human placenta were activated by dATP to form apoptosome; the formation of apoptosome was markedly inhibited by reducing reagents such as DTT or reduced glutathione (GSH), whereas hydrogen peroxide prevented this inhibition. We also found that apoptosome formation was substantially impaired by GSH-pretreated Apaf-1, but not GSH-pretreated procaspase-9 or GSH-pretreated cytochrome c. Collectively, these results suggest that ROS plays an essential role in apoptosome formation by oxidizing Apaf-1 and the subsequent activation of caspase-9 and -3.  (日)    [継承]
キーワード (推奨): 1. (英) Antigens, CD95 (日) (読) [継承]
2. (英) Apoptosis (日) (読) [継承]
3. (英) Apoptotic Protease-Activating Factor 1 (日) (読) [継承]
4. (英) Caspase 3 (日) (読) [継承]
5. (英) Caspase 9 (日) (読) [継承]
6. (英) Caspases (日) (読) [継承]
7. (英) Cytochromes c (日) (読) [継承]
8. (英) Humans (日) (読) [継承]
9. (英) Hydrogen Peroxide (日) (読) [継承]
10. (英) Jurkat Cells (日) (読) [継承]
11. (英) Mitochondria (日) (読) [継承]
12. (英) Permeability (日) (読) [継承]
13. (英) Proteins (日) (読) [継承]
14. (英) Reactive Oxygen Species (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Immunology ([The American Association of Immunologists])
(pISSN: 0022-1767, eISSN: 1550-6606)

ISSN (任意): 0022-1767
ISSN: 0022-1767 (pISSN: 0022-1767, eISSN: 1550-6606)
Title: Journal of immunology (Baltimore, Md. : 1950)
Title(ISO): J Immunol
Publisher: American Association of Immunologists
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年月日 (必須): 西暦 2004年 7月 1日 (平成 16年 7月 1日) [継承]
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PMID (任意): 15210786    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) PublicationType: Journal Article  (日)    [継承]
2.(英) PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Tsutomu Sato, Takuro Machida, Sho Takahashi, Satoshi Iyama, Yasushi Sato, Kageaki Kuribayashi, Kohichi Takada, Takatomi Oku, Yutaka Kawano, Tetsuro Okamoto, Rishu Takimoto, Takuya Matsunaga, Tetsuji Takayama, Minoru Takahashi, Junji Kato and Yoshiro Niitsu : Fas-mediated apoptosome formation is dependent on reactive oxygen species derived from mitochondrial permeability transition in Jurkat cells., The Journal of Immunology, Vol.173, No.1, 285-296, 2004.
欧文冊子 ● Tsutomu Sato, Takuro Machida, Sho Takahashi, Satoshi Iyama, Yasushi Sato, Kageaki Kuribayashi, Kohichi Takada, Takatomi Oku, Yutaka Kawano, Tetsuro Okamoto, Rishu Takimoto, Takuya Matsunaga, Tetsuji Takayama, Minoru Takahashi, Junji Kato and Yoshiro Niitsu : Fas-mediated apoptosome formation is dependent on reactive oxygen species derived from mitochondrial permeability transition in Jurkat cells., The Journal of Immunology, Vol.173, No.1, 285-296, 2004.

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