| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○カテゴリ (推奨): |
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| ○著者 (必須): | 1. | (英) Akiyama Toshihiro (日) (読)
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| 2. | (英) Inoko Akihito (日) (読)
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| 3. | (英) Kaji Yuichi (日) (読)
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| 4. | 米村 重信 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.生理系.細胞生物学])
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| 5. | (英) Kakiguchi Kisa (日) (読)
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| 6. | (英) Segawa Hiroki (日) (読)
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| 7. | (英) Ishitsuka Kei (日) (読)
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| 8. | (英) Yoshida Masaki (日) (読)
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| 9. | (英) Numata Osamu (日) (読)
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| 10. | (英) Leproux Philippe (日) (読)
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| 11. | (英) Couderc Vincent (日) (読)
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| 12. | (英) Oshika Tetsuro (日) (読)
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| 13. | (英) Kano Hideaki (日) (読)
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| ○題名 (必須): | □ | (英) SHG-specificity of cellular Rootletin filaments enables naïve imaging with universal conservation. (日)
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| ○要約 (任意): | □ | (英) Despite growing demand for truly naïve imaging, label-free observation of cilium-related structure remains challenging, and validation of the pertinent molecules is correspondingly difficult. In this study, in retinas and cultured cells, we distinctively visualized Rootletin filaments in rootlets in the second harmonic generation (SHG) channel, integrated in custom coherent nonlinear optical microscopy (CNOM) with a simple, compact, and ultra-broadband supercontinuum light source. This SHG signal was primarily detected on rootlets of connecting cilia in the retinal photoreceptor and was validated by colocalization with anti-Rootletin staining. Transfection of cells with Rootletin fragments revealed that the SHG signal can be ascribed to filaments assembled from the R234 domain, but not to cross-striations assembled from the R123 domain. Consistent with this, Rootletin-depleted cells lacked SHG signal expected as centrosome linker. As a proof of concept, we confirmed that similar fibrous SHG was observed even in unicellular ciliates. These findings have potential for broad applications in clinical diagnosis and biophysical experiments with various organisms. (日)
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| ○キーワード (推奨): |
| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Scientific Reports ([Nature Publishing Group])
(eISSN: 2045-2322)
| ○ISSN (任意): | □ | 2045-2322
ISSN: 2045-2322
(eISSN: 2045-2322) Title: Scientific reportsTitle(ISO): Sci RepPublisher: Nature Portfolio (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 7
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| ○号 (必須): | □ |
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| ○頁 (必須): | □ | 39967 39967
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2017年 1月 6日 (平成 29年 1月 6日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1038/srep39967 (→Scopusで検索)
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| ○PMID (任意): | □ | 28059168 (→Scopusで検索)
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| ○機関リポジトリ : | □ | 112380
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| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1038/srep39967 (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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