| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○著者 (必須): | 1. | (英) Maki K (日) (読)
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| 2. | (英) Han SW (日) (読)
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| 3. | (英) Hirano Y (日) (読)
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| 4. | (英) Yonemura S (日) (読)
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| 5. | (英) Hakoshima T (日) (読)
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| 6. | (英) Adachi T. (日) (読)
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| 7. | 米村 重信 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.生理系.細胞生物学])
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| ○題名 (必須): | □ | (英) Mechano-adaptive sensory mechanism of -catenin under tension. (日)
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| ○要約 (任意): | □ | (英) The contractile forces in individual cells drive the tissue processes, such as morphogenesis and wound healing, and maintain tissue integrity. In these processes, α-catenin molecule acts as a tension sensor at cadherin-based adherens junctions (AJs), accelerating the positive feedback of intercellular tension. Under tension, α-catenin is activated to recruit vinculin, which recruits actin filaments to AJs. In this study, we revealed how α-catenin retains its activated state while avoiding unfolding under tension. Using single-molecule force spectroscopy employing atomic force microscopy (AFM), we found that mechanically activated α-catenin fragment had higher mechanical stability than a non-activated fragment. The results of our experiments using mutated and segmented fragments showed that the key intramolecular interactions acted as a conformational switch. We also found that the conformation of α-catenin was reinforced by vinculin binding. We demonstrate that α-catenin adaptively changes its conformation under tension to a stable intermediate state, binds to vinculin, and finally settles into a more stable state reinforced by vinculin binding. Our data suggest that the plastic characteristics of α-catenin, revealed in response to both mechanical and biochemical cues, enable the functional-structural dynamics at the cellular and tissue levels. (日)
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| ○キーワード (推奨): |
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| ○誌名 (必須): | □ | Scientific Reports ([Nature Publishing Group])
(eISSN: 2045-2322)
| ○ISSN (任意): | □ | 2045-2322
ISSN: 2045-2322
(eISSN: 2045-2322) Title: Scientific reportsTitle(ISO): Sci RepPublisher: Nature Portfolio (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 6
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| ○号 (必須): | □ |
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| ○頁 (必須): | □ | 24878 24878
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| ○年月日 (必須): | □ | 西暦 2016年 4月 25日 (平成 28年 4月 25日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1038/srep24878 (→Scopusで検索)
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| ○PMID (任意): | □ | 27109499 (→Scopusで検索)
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| ○機関リポジトリ : | □ | 112377
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| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1038/srep24878 (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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| 4. | (英) OtherID: PMC4843013 (日)
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