| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Iwasaki Y (日) (読)
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| 2. | (英) Sugita S (日) (読)
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| 3. | (英) Mandai M (日) (読)
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| 4. | (英) Yonemura S (日) (読)
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| 5. | (英) Onishi A (日) (読)
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| 6. | (英) Ito S (日) (読)
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| 7. | (英) Mochizuki M (日) (読)
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| 8. | (英) Ohno-Matsui K (日) (読)
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| 9. | (英) Takahashi M. (日) (読)
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| 10. | 米村 重信 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.生理系.細胞生物学])
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| ○題名 (必須): | □ | (英) Differentiation/Purification Protocol for Retinal Pigment Epithelium from Mouse Induced Pluripotent Stem Cells as a Research Tool. (日)
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| ○要約 (任意): | □ | (英) To establish a novel protocol for differentiation of retinal pigment epithelium (RPE) with high purity from mouse induced pluripotent stem cells (iPSC). Retinal progenitor cells were differentiated from mouse iPSC, and RPE differentiation was then enhanced by activation of the Wnt signaling pathway, inhibition of the fibroblast growth factor signaling pathway, and inhibition of the Rho-associated, coiled-coil containing protein kinase signaling pathway. Expanded pigmented cells were purified by plate adhesion after Accutase® treatment. Enriched cells were cultured until they developed a cobblestone appearance with cuboidal shape. The characteristics of iPS-RPE were confirmed by gene expression, immunocytochemistry, and electron microscopy. Functions and immunologic features of the iPS-RPE were also evaluated. We obtained iPS-RPE at high purity (approximately 98%). The iPS-RPE showed apical-basal polarity and cellular structure characteristic of RPE. Expression levels of several RPE markers were lower than those of freshly isolated mouse RPE but comparable to those of primary cultured RPE. The iPS-RPE could form tight junctions, phagocytose photoreceptor outer segments, express immune antigens, and suppress lymphocyte proliferation. We successfully developed a differentiation/purification protocol to obtain mouse iPS-RPE. The mouse iPS-RPE can serve as an attractive tool for functional and morphological studies of RPE. (日)
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| ○キーワード (推奨): | 1. | (英) Animals (日) (読)
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| 2. | (英) Cell Communication (日) (読)
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| 3. | (英) Cell Culture Techniques (日) (読)
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| 4. | (英) Cell Differentiation (日) (読)
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| 5. | (英) Cell Proliferation (日) (読)
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| 6. | (英) Cells, Cultured (日) (読)
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| 7. | (英) Collagenases (日) (読)
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| 8. | (英) Embryonic Stem Cells (日) (読)
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| 9. | (英) Induced Pluripotent Stem Cells (日) (読)
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| 10. | (英) Lymphocytes (日) (読)
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| 11. | (英) Mice (日) (読)
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| 12. | (英) Peptide Hydrolases (日) (読)
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| 13. | (英) Phagocytosis (日) (読)
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| 14. | (英) Retinal Pigment Epithelium (日) (読)
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| 15. | (英) Rod Cell Outer Segment (日) (読)
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| 16. | (英) Signal Transduction (日) (読)
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| 17. | (英) rho-Associated Kinases (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | PLoS ONE (Public Library of Science)
(eISSN: 1932-6203)
| ○ISSN (任意): | □ | 1932-6203
ISSN: 1932-6203
(eISSN: 1932-6203) Title: PloS oneTitle(ISO): PLoS OnePublisher: PLOS (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 11
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| ○号 (必須): | □ | 7
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| ○頁 (必須): | □ | e0158282 e0158282
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2016年 7月 6日 (平成 28年 7月 6日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1371/journal.pone.0158282 (→Scopusで検索)
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| ○PMID (任意): | □ | 27385038 (→Scopusで検索)
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| ○機関リポジトリ : | □ | 112340
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| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1371/journal.pone.0158282 (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) OtherID: PMC4934919 (日)
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