| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): |
| ○共著種別 (推奨): |
| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Okada Yasuyuki (日) 岡田 泰行 (読) おかだ やすゆき
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): | □ | ****
| [ユーザ] |
| [継承] |
| 2. | 木村 哲夫
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 3. | 中川 忠彦
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 4. | 岡本 耕一 ([徳島大学.病院.診療科.内科.消化器内科])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 5. | (英) Fukuya Akira (日) 福家 慧 (読) ふくや あきら
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): | □ | ****
| [ユーザ] |
| [継承] |
| 6. | 郷司 敬洋
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 7. | (英) Fujimoto Shota (日) 藤本 将太 (読) ふじもと しょうた
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): | □ | ****
| [ユーザ] |
| [継承] |
| 8. | 曽我部 正弘 ([徳島大学.キャンパスライフ健康支援センター])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 9. | 宮本 弘志 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.内科系.消化器内科学]/[徳島大学.病院.診療科.内科.消化器内科])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 10. | 六車 直樹
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 11. | (英) Tsuji Yasushi (日) (読)
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 12. | 岡久 稔也 ([徳島大学.大学院医歯薬学研究部.医学域.連携研究部門(医学域).寄附講座系(医学域).地域総合医療学]/[徳島大学.病院.診療科.内科.消化器内科])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| 13. | 高山 哲治 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.内科系.消化器内科学])
| ○役割 (任意): |
| ○貢献度 (任意): |
| ○学籍番号 (推奨): |
| [継承] |
| ○題名 (必須): | □ | (英) EGFR Down-regulation After anti-EGFR Therapy Predicts the Anti-tumor Effect in Colorectal Cancer. (日)
| [継承] |
| ○副題 (任意): |
| ○要約 (任意): | □ | (英) Anti-EGFR mAb is reported to induce EGFR internalization in colorectal cancer cells. However, the biological relevance of EGFR internalization with anti-EGFR mAb is unknown. Therefore, the relevance of EGFR downregulation with anti-EGFR mAb to antitumor activity in colorectal cancer cells was investigated. Quantification of EGFR on the cell surface before cetuximab treatment was assessed by flow cytometry, and its growth-inhibitory effects were measured by Trypan blue exclusion, in 10 wild-type colorectal cancer cell lines, but there was no significant correlation between EGFR number and its growth-inhibitory effect. However, a significant correlation existed between the percentage decrease in the number of EGFRs after cetuximab treatment and its growth-inhibitory effect in those cell lines. Treatment with TGFα, a ligand for EGFR, induced EGFR internalization in colorectal cancer cells, but most EGFRs subsequently recycled to the cell surface, consistent with previous studies. While cetuximab treatment induced EGFR internalization, most receptors subsequently translocated into the late endosome, leading to lysosomal degradation, as revealed by immunoblotting and double immunofluorescence. Cetuximab-sensitive colorectal cancer cells showed greater EGFR internalization, stronger cell growth inhibition, and more augmented apoptotic signals than nonsensitive cells. IHC for EGFR, performed using an EGFR pharmDx Kit (mouse anti-human EGFR mAb clone 2-18C9), in clinical specimens before and after anti-EGFR mAb therapy in 13 colorectal cancer patients showed a significant correlation between the response to anti-EGFR mAb and decreased staining after therapy. This report clearly demonstrates that anti-EGFR mAb facilitates internalization and subsequent degradation of EGFRs in lysosomes, which is an important determinant of the efficacy of anti-EGFR mAb treatment for colorectal cancer. . (日)
| [継承] |
| ○キーワード (推奨): | 1. | (英) Adult (日) (読)
| [継承] |
| 2. | (英) Aged (日) (読)
| [継承] |
| 3. | (英) Antineoplastic Agents, Immunological (日) (読)
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| 4. | (英) Caco-2 Cells (日) (読)
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| 5. | (英) Cell Line, Tumor (日) (読)
| [継承] |
| 6. | (英) Cell Survival (日) (読)
| [継承] |
| 7. | (英) Cetuximab (日) (読)
| [継承] |
| 8. | (英) Colorectal Neoplasms (日) (読)
| [継承] |
| 9. | (英) Down-Regulation (日) (読)
| [継承] |
| 10. | (英) Endosomes (日) (読)
| [継承] |
| 11. | (英) Female (日) (読)
| [継承] |
| 12. | (英) HCT116 Cells (日) (読)
| [継承] |
| 13. | (英) HT29 Cells (日) (読)
| [継承] |
| 14. | (英) Humans (日) (読)
| [継承] |
| 15. | (英) Male (日) (読)
| [継承] |
| 16. | (英) Middle Aged (日) (読)
| [継承] |
| 17. | (英) Proto-Oncogene Proteins B-raf (日) (読)
| [継承] |
| 18. | (英) Receptor, Epidermal Growth Factor (日) (読)
| [継承] |
| 19. | (英) Transforming Growth Factor alpha (日) (読)
| [継承] |
| 20. | (英) ras Proteins (日) (読)
| [継承] |
| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Molecular Cancer Research : MCR ([American Association for Cancer Research])
(pISSN: 1541-7786, eISSN: 1557-3125)
| ○ISSN (任意): | □ | 1557-3125
ISSN: 1541-7786
(pISSN: 1541-7786, eISSN: 1557-3125) Title: Molecular cancer research : MCRTitle(ISO): Mol Cancer ResPublisher: American Association for Cancer Research (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
| [継承] |
| ○巻 (必須): | □ | 15
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| ○号 (必須): | □ | 10
| [継承] |
| ○頁 (必須): | □ | 1445 1454
| [継承] |
| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2017年 10月 初日 (平成 29年 10月 初日)
| [継承] |
| ○URL (任意): |
| ○DOI (任意): | □ | 10.1158/1541-7786.MCR-16-0383 (→Scopusで検索)
| [継承] |
| ○PMID (任意): | □ | 28698359 (→Scopusで検索)
| [継承] |
| ○CRID (任意): |
| ○WOS (任意): |
| ○Scopus (任意): |
| ○評価値 (任意): |
| ○被引用数 (任意): |
| ○指導教員 (推奨): |
| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1158/1541-7786.MCR-16-0383 (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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