『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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登録内容 (EID=329476)

EID=329476EID:329476, Map:0, LastModified:2017年8月17日(木) 16:53:43, Operator:[藤野 裕道], Avail:TRUE, Censor:0, Owner:[藤野 裕道], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1.藤野 裕道 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.生命薬学系.生命薬理学])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2. (英) West Kimberly A (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Regan John W (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Phosphorylation of glycogen synthase kinase-3 and stimulation of T-cell factor signaling following activation of EP2 and EP4 prostanoid receptors by prostaglandin E2.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Recently we have shown that the FP(B) prostanoid receptor, a G-protein-coupled receptor that couples to Galpha(q), activates T-cell factor (Tcf)/lymphoid enhancer factor (Lef)-mediated transcriptional activation (Fujino, H., and Regan, J. W. (2001) J. Biol. Chem. 276, 12489-12492). We now report that the EP(2) and EP(4) prostanoid receptors, which couple to Galpha(s), also activate Tcf/Lef signaling. By using a Tcf/Lef-responsive luciferase reporter gene, transcriptional activity was stimulated approximately 10-fold over basal by 1 h of treatment with prostaglandin E(2) (PGE(2)) in HEK cells that were stably transfected with the human EP(2) and EP(4) receptors. This stimulation of reporter gene activity was accompanied by a PGE(2)-dependent increase in the phosphorylation of both glycogen synthase kinase-3 (GSK-3) and Akt kinase. H-89, an inhibitor of protein kinase A (PKA), completely blocked the agonist-dependent phosphorylation of GSK-3 in both EP(2)- and EP(4)-expressing cells. However, H-89 pretreatment only blocked PGE(2)-stimulated Lef/Tcf reporter gene activity by 20% in EP(4)-expressing cells compared with 65% inhibition in EP(2)-expressing cells. On the other hand wortmannin, an inhibitor of phosphatidylinositol 3-kinase, had the opposite effect and inhibited PGE(2)-stimulated reporter gene activity to a much greater extent in EP(4)-expressing cells as compared with EP(2)-expressing cells. These findings indicate that the activation of Tcf/Lef signaling by EP(2) receptors occurs primarily through a PKA-dependent pathway, whereas EP(4) receptors activate Tcf/Lef signaling mainly through a phosphatidylinositol 3-kinase-dependent pathway. This is the first indication of a fundamental difference in the signaling potential of EP(2) and EP(4) prostanoid receptors.  (日)    [継承]
キーワード (推奨): 1. (英) Androstadienes (日) (読) [継承]
2. (英) Binding, Competitive (日) (読) [継承]
3. (英) Blotting, Western (日) (読) [継承]
4. (英) Calcium-Calmodulin-Dependent Protein Kinases (日) (読) [継承]
5. (英) Cell Differentiation (日) (読) [継承]
6. (英) Cell Division (日) (読) [継承]
7. (英) Cell Line (日) (読) [継承]
8. (英) Cyclic AMP-Dependent Protein Kinases (日) (読) [継承]
9. (英) DNA-Binding Proteins (日) (読) [継承]
10. (英) Dinoprostone (日) (読) [継承]
11. (英) Dose-Response Relationship, Drug (日) (読) [継承]
12. (英) Enzyme Inhibitors (日) (読) [継承]
13. (英) Genes, Reporter (日) (読) [継承]
14. (英) Glycogen Synthase Kinase 3 (日) (読) [継承]
15. (英) Glycogen Synthase Kinases (日) (読) [継承]
16. (英) Humans (日) (読) [継承]
17. (英) Isoquinolines (日) (読) [継承]
18. (英) Luciferases (日) (読) [継承]
19. (英) Lymphoid Enhancer-Binding Factor 1 (日) (読) [継承]
20. (英) Phosphatidylinositol 3-Kinases (日) (読) [継承]
21. (英) Phosphorylation (日) (読) [継承]
22. (英) Protein Binding (日) (読) [継承]
23. (英) Radioligand Assay (日) (読) [継承]
24. (英) Receptors, Prostaglandin E (日) (読) [継承]
25. (英) Receptors, Prostaglandin E, EP2 Subtype (日) (読) [継承]
26. (英) Receptors, Prostaglandin E, EP4 Subtype (日) (読) [継承]
27. (英) Signal Transduction (日) (読) [継承]
28. (英) Sulfonamides (日) (読) [継承]
29. (英) Time Factors (日) (読) [継承]
30. (英) Transcription Factors (日) (読) [継承]
31. (英) Transcription, Genetic (日) (読) [継承]
32. (英) Transfection (日) (読) [継承]
発行所 (推奨):
誌名 (必須): The Journal of Biological Chemistry ([The American Society for Biochemistry and Molecular Biology])
(pISSN: 0021-9258, eISSN: 1083-351X)

ISSN (任意): 0021-9258
ISSN: 0021-9258 (pISSN: 0021-9258, eISSN: 1083-351X)
Title: The Journal of biological chemistry
Title(ISO): J Biol Chem
Publisher: American Society for Biochemistry and Molecular Biology
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 277 [継承]
(必須): 4 [継承]
(必須): 2614 2619 [継承]
都市 (任意):
年月日 (必須): 西暦 2001年 11月 12日 (平成 13年 11月 12日) [継承]
URL (任意):
DOI (任意): 10.1074/jbc.M109440200    (→Scopusで検索) [継承]
PMID (任意): 11706038    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Hiromichi Fujino, A Kimberly West and W John Regan : Phosphorylation of glycogen synthase kinase-3 and stimulation of T-cell factor signaling following activation of EP2 and EP4 prostanoid receptors by prostaglandin E2., The Journal of Biological Chemistry, Vol.277, No.4, 2614-2619, 2001.
欧文冊子 ● Hiromichi Fujino, A Kimberly West and W John Regan : Phosphorylation of glycogen synthase kinase-3 and stimulation of T-cell factor signaling following activation of EP2 and EP4 prostanoid receptors by prostaglandin E2., The Journal of Biological Chemistry, Vol.277, No.4, 2614-2619, 2001.

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