『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
ID: Pass:

登録内容 (EID=329475)

EID=329475EID:329475, Map:0, LastModified:2018年12月18日(火) 14:21:13, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[藤野 裕道], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Anthony Todd L (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2.藤野 裕道 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.生命薬学系.生命薬理学])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Pierce Kristen L (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Yool Andrea J (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
5. (英) Regan John W (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) Differential regulation of Ca(2+)-dependent Cl- currents by FP prostanoid receptor isoforms in Xenopus oocytes.  (日)    [継承]
副題 (任意):
要約 (任意): (英) The FP(A) and FP(B) prostanoid receptor isoforms are G-protein-coupled receptors that are activated by prostaglandin F(2alpha) (PGF(2alpha)). Differences in their carboxyl termini prompted us to examine the intracellular calcium (Ca(2+)) signaling of these receptor isoforms using the Xenopus oocyte expression system. Protein expression was determined by immunofluorescence microscopy and whole cell binding with [3H]PGF(2alpha). Positive immunolabeling was observed on the outer membranes of oocytes expressing FLAG-tagged FP receptor isoforms, but not on control (water-injected) oocytes. Intracellular signaling was examined using a two-electrode voltage clamp. Specific whole-cell binding was also detected for both receptor isoforms. Bath application of 10 microM PGF(2alpha) to FP(A)-expressing oocytes produced a chloride (Cl-) current response similar to that of an injection of inositol 1,4,5-trisphosphate (InsP(3)) (5.76+/-0.6 microA, peak current; N=23) that returned to control levels within 25 min. In FP(B)-expressing oocytes the activation of the Cl- current was delayed or completely absent (1.38+/-0.2 microA, peak current; N=18). Control oocytes were not responsive to the application of PGF(2alpha) (0.87+/-0.1 microA, peak current; N=10). Activation of Cl- currents for both FP receptor isoforms was dependent upon intracellular Ca(2+) stores as a 30-min pretreatment with thapsigargin (1 microM; N=5) blocked the PGF(2alpha) induction of the Cl- current. These data indicate that the FP prostanoid receptor isoforms differ in their ability to activate Ca(2+)-dependent Cl- channels when expressed in Xenopus oocytes. The difference appears to be in the ability of the two FP prostanoid receptor isoforms to mobilize intracellular calcium.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Calcium (日) (読) [継承]
3. (英) Chloride Channels (日) (読) [継承]
4. (英) Dinoprost (日) (読) [継承]
5. (英) Enzyme Inhibitors (日) (読) [継承]
6. (英) Oocytes (日) (読) [継承]
7. (英) Phosphoric Monoester Hydrolases (日) (読) [継承]
8. (英) Protein Isoforms (日) (読) [継承]
9. (英) Receptors, Prostaglandin (日) (読) [継承]
10. (英) Xenopus laevis (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Biochemical Pharmacology ([Elsevier Science])
(pISSN: 0006-2952, eISSN: 1873-2968)

ISSN (任意): 0006-2952
ISSN: 0006-2952 (pISSN: 0006-2952, eISSN: 1873-2968)
Title: Biochemical pharmacology
Title(ISO): Biochem Pharmacol
Publisher: Elsevier BV
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 63 [継承]
(必須): 10 [継承]
(必須): 1797 1806 [継承]
都市 (任意):
年月日 (必須): 西暦 2002年 5月 15日 (平成 14年 5月 15日) [継承]
URL (任意):
DOI (任意): 10.1016/S0006-2952(02)00953-X    (→Scopusで検索) [継承]
PMID (任意): 12034364    (→Scopusで検索) [継承]
NAID (任意):
WOS (任意):
Scopus (任意):
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意): 1.(英) PublicationType: Journal Article  (日)    [継承]
2.(英) PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]
3.(英) PublicationType: Research Support, U.S. Gov't, P.H.S.  (日)    [継承]

標準的な表示

和文冊子 ● L Todd Anthony, Hiromichi Fujino, L Kristen Pierce, J Andrea Yool and W John Regan : Differential regulation of Ca(2+)-dependent Cl- currents by FP prostanoid receptor isoforms in Xenopus oocytes., Biochemical Pharmacology, Vol.63, No.10, 1797-1806, 2002.
欧文冊子 ● L Todd Anthony, Hiromichi Fujino, L Kristen Pierce, J Andrea Yool and W John Regan : Differential regulation of Ca(2+)-dependent Cl- currents by FP prostanoid receptor isoforms in Xenopus oocytes., Biochemical Pharmacology, Vol.63, No.10, 1797-1806, 2002.

関連情報

Number of session users = 5, LA = 0.26, Max(EID) = 383935, Max(EOID) = 1026852.