『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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EID=329455EID:329455, Map:0, LastModified:2017年8月17日(木) 16:46:15, Operator:[藤野 裕道], Avail:TRUE, Censor:0, Owner:[藤野 裕道], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Taniguchi Tomoko (日) (読)
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[継承]
2. (英) Shimizu Masaya (日) (読)
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[継承]
3. (英) Nakamura Hiroyuki (日) (読)
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学籍番号 (推奨):
[継承]
4. (英) Hirabayashi Tetsuya (日) (読)
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[継承]
5.藤野 裕道 ([徳島大学.大学院医歯薬学研究部.薬学域.薬科学部門.生命薬学系.生命薬理学])
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学籍番号 (推奨):
[継承]
6. (英) Murayama Toshihiko (日) (読)
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学籍番号 (推奨):
[継承]
題名 (必須): (英) Hydrogen peroxide-induced arachidonic acid release in L929 cells; roles of Src, protein kinase C and cytosolic phospholipase A2alpha.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Hydrogen peroxide (H(2)O(2)) stimulates the release of arachidonic acid from cells, but the signaling mechanism(s) involved remains to be elucidated. We investigated the roles of alpha-type cytosolic phospholipase A(2) (cPLA(2)alpha), Src family kinases (Src) and protein kinase C (PKC) in the release of arachidonic acid from L929 cells (a murine fibroblast cell line), C12 cells (a variant of L929 that lacks cPLA(2)alpha) and a stable clone of C12 cells expressing cPLA(2)alpha (C12-cPLA(2)alpha cells). In the presence of 10 muM A23187, 100 nM phorbol myristate acetate (PMA) and 1 mM H(2)O(2) synergistically stimulated arachidonic acid release from L929 cells and C12-cPLA(2)alpha cells, and to a much lesser extent from C12 cells. The reagents alone and co-treatment with PMA and H(2)O(2) without A23187 had marginal effects. No arachidonic acid was released by PMA/A23187 or H(2)O(2)/A23187 in CaCl(2)-free buffer and the release was inhibited by a selective cPLA(2)alpha inhibitor (3 microM pyrrophenone). Addition of 10 microM H(2)O(2), which did not stimulate arachidonic acid release with A23187, enhanced the response to PMA/A23187. The release induced by PMA/A23187 and by H(2)O(2)/A23187 was significantly inhibited by a PKC inhibitor (10 microM GF109203X) and in PKC-depleted cells, and by a Src inhibitor (2 microM PP2). The phosphorylation of extracellular signal-regulated kinase 1/2 induced by PMA/A23187 and H(2)O(2)/A23187 was significantly decreased by inhibitors of PKC and Src. These findings suggest that H(2)O(2) with Ca(2+) stimulates arachidonic acid release via cPLA(2)alpha in a Src- and PKC-dependent manner in L929 cells. The role of cross-talk between Src and PKC in arachidonic acid release is discussed.  (日)    [継承]
キーワード (推奨): 1. (英) Animals (日) (読) [継承]
2. (英) Arachidonic Acid (日) (読) [継承]
3. (英) Calcimycin (日) (読) [継承]
4. (英) Calcium (日) (読) [継承]
5. (英) Cell Line, Tumor (日) (読) [継承]
6. (英) Cytosol (日) (読) [継承]
7. (英) Dose-Response Relationship, Drug (日) (読) [継承]
8. (英) Enzyme Inhibitors (日) (読) [継承]
9. (英) Fibroblasts (日) (読) [継承]
10. (英) Group IV Phospholipases A2 (日) (読) [継承]
11. (英) Humans (日) (読) [継承]
12. (英) Hydrogen Peroxide (日) (読) [継承]
13. (英) Indoles (日) (読) [継承]
14. (英) Ionophores (日) (読) [継承]
15. (英) Maleimides (日) (読) [継承]
16. (英) Mice (日) (読) [継承]
17. (英) Phospholipases A (日) (読) [継承]
18. (英) Protein Kinase C (日) (読) [継承]
19. (英) Protein Kinase C-alpha (日) (読) [継承]
20. (英) Protein Transport (日) (読) [継承]
21. (英) Pyrimidines (日) (読) [継承]
22. (英) Pyrrolidines (日) (読) [継承]
23. (英) Signal Transduction (日) (読) [継承]
24. (英) Tetradecanoylphorbol Acetate (日) (読) [継承]
25. (英) Transfection (日) (読) [継承]
26. (英) src-Family Kinases (日) (読) [継承]
発行所 (推奨):
誌名 (必須): European Journal of Pharmacology ([Elsevier])
(pISSN: 0014-2999, eISSN: 1879-0712)

ISSN (任意): 0014-2999
ISSN: 0014-2999 (pISSN: 0014-2999, eISSN: 1879-0712)
Title: European journal of pharmacology
Title(ISO): Eur J Pharmacol
Publisher: Elsevier BV
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(必須): 546 [継承]
(必須): 1-3 [継承]
(必須): 1 10 [継承]
都市 (任意):
年月日 (必須): 西暦 2006年 7月 5日 (平成 18年 7月 5日) [継承]
URL (任意):
DOI (任意): 10.1016/j.ejphar.2006.06.071    (→Scopusで検索) [継承]
PMID (任意): 16914136    (→Scopusで検索) [継承]
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備考 (任意): 1.(英) PublicationType: Comparative Study  (日)    [継承]
2.(英) PublicationType: Journal Article  (日)    [継承]
3.(英) PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Tomoko Taniguchi, Masaya Shimizu, Hiroyuki Nakamura, Tetsuya Hirabayashi, Hiromichi Fujino and Toshihiko Murayama : Hydrogen peroxide-induced arachidonic acid release in L929 cells; roles of Src, protein kinase C and cytosolic phospholipase A2alpha., European Journal of Pharmacology, Vol.546, No.1-3, 1-10, 2006.
欧文冊子 ● Tomoko Taniguchi, Masaya Shimizu, Hiroyuki Nakamura, Tetsuya Hirabayashi, Hiromichi Fujino and Toshihiko Murayama : Hydrogen peroxide-induced arachidonic acid release in L929 cells; roles of Src, protein kinase C and cytosolic phospholipase A2alpha., European Journal of Pharmacology, Vol.546, No.1-3, 1-10, 2006.

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