『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔微生物学 (2015年4月1日〜) [継承]
著者 (必須): 1. (英) Viducic Darija (日) (読)
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2.村上 圭史
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3.天羽 崇
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学籍番号 (推奨): **** [ユーザ]
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4.小野 恒子
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5.三宅 洋一郎
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題名 (必須): (英) RpoN Modulates Carbapenem Tolerance in Pseudomonas aeruginosa through Pseudomonas Quinolone Signal and PqsE.  (日)    [継承]
副題 (任意):
要約 (任意): (英) The ability of Pseudomonas aeruginosa to rapidly modulate its response to antibiotic stress and persist in the presence of antibiotics is closely associated with the process of cell-to-cell signaling. The alternative sigma factor RpoN ( (54)) is involved in the regulation of quorum sensing (QS) and plays an important role in the survival of stationary-phase cells in the presence of carbapenems. Here, we demonstrate that a poN mutant grown in nutrient-rich medium has increased expression of pqsA, pqsH, and pqsR throughout growth, resulting in the increased production of the Pseudomonas quinolone signal (PQS). The link between pqsA and its role in carbapenem tolerance was studied using a poN qsA mutant, in which the carbapenem-tolerant phenotype of the poN mutant was abolished. In addition, we demonstrate that another mechanism leading to carbapenem tolerance in the poN mutant is mediated through pqsE Exogenously supplied PQS abolished the biapenem-sensitive phenotype of the poN qsA mutant, and overexpression of pqsE failed to alter the susceptibility of the poN qsA mutant to biapenem. The mutations in the poN hlR mutant and the poN qsH mutant led to susceptibility to biapenem. Comparison of the changes in the expression of the genes involved in QS in wild-type PAO1 with their expression in the poN mutant and the poN mutant-derived strains demonstrated the regulatory effect of RpoN on the transcript levels of rhlR, vqsR, and rpoS The findings of this study demonstrate that RpoN negatively regulates the expression of PQS in nutrient-rich medium and provide evidence that RpoN interacts with pqsA, pqsE, pqsH, and rhlR in response to antibiotic stress.  (日)    [継承]
キーワード (推奨): 1. (英) Bacterial Proteins (日) (読) [継承]
2. (英) Carbapenems (日) (読) [継承]
3. (英) Drug Resistance, Bacterial (日) (読) [継承]
4. (英) Gene Expression Regulation, Bacterial (日) (読) [継承]
5. (英) Microbial Sensitivity Tests (日) (読) [継承]
6. (英) Mutation (日) (読) [継承]
7.緑膿菌 (Pseudomonas aeruginosa) [継承]
8. (英) Quinolones (日) (読) [継承]
9.クォーラムセンシング (quorum sensing) [継承]
10. (英) Sigma Factor (日) (読) [継承]
11.シグナル伝達 (signal transduction) [継承]
12. (英) Trans-Activators (日) (読) [継承]
発行所 (推奨):
誌名 (必須): Antimicrobial Agents and Chemotherapy ([アメリカ微生物学会])
(pISSN: 0066-4804, eISSN: 1098-6596)

ISSN (任意): 1098-6596
ISSN: 0066-4804 (pISSN: 0066-4804, eISSN: 1098-6596)
Title: Antimicrobial agents and chemotherapy
Title(ISO): Antimicrob Agents Chemother
Publisher: American Society for Microbiology
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
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(必須): 60 [継承]
(必須): 10 [継承]
(必須): 5752 5764 [継承]
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年月日 (必須): 西暦 2016年 9月 23日 (平成 28年 9月 23日) [継承]
URL (任意):
DOI (任意): 10.1128/AAC.00260-16    (→Scopusで検索) [継承]
PMID (任意): 27431228    (→Scopusで検索) [継承]
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Scopus (任意): 2-s2.0-84992643511 [継承]
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備考 (任意): 1.(英) Article.ELocationID: 10.1128/AAC.00260-16  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]

標準的な表示

和文冊子 ● Darija Viducic, Keiji Murakami, Takashi Amoh, Tsuneko Ono and Yoichiro Miyake : RpoN Modulates Carbapenem Tolerance in Pseudomonas aeruginosa through Pseudomonas Quinolone Signal and PqsE., Antimicrobial Agents and Chemotherapy, Vol.60, No.10, 5752-5764, 2016.
欧文冊子 ● Darija Viducic, Keiji Murakami, Takashi Amoh, Tsuneko Ono and Yoichiro Miyake : RpoN Modulates Carbapenem Tolerance in Pseudomonas aeruginosa through Pseudomonas Quinolone Signal and PqsE., Antimicrobial Agents and Chemotherapy, Vol.60, No.10, 5752-5764, 2016.

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