| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○カテゴリ (推奨): | □ | 研究
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| ○著者 (必須): | 1. | (英) Irie, Atsushi (日) (読)
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| 2. | 山本 圭 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.応用生命科学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命コース])
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| 3. | (英) Miki, Yoshimi (日) (読)
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| 4. | (英) Murakami, Makoto (日) (読)
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| ○題名 (必須): | □ | (英) Phosphatidylethanolamine dynamics are required for osteoclast fusion. (日)
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| ○要約 (任意): | □ | (英) Osteoclasts, responsible for bone resorption, are multinucleated cells formed by cell-cell fusion of mononuclear pre-osteoclasts. Although osteoclast fusion is a pivotal step for osteoclastogenesis, little is known about the mechanism involved. To clarify the underlying process, we investigated dynamics of membrane phospholipids during osteoclastogenesis in vitro. We found that the cellular content of phospholipids, phosphatidylethanolamine (PE) in particular, was increased during osteoclast differentiation. Furthermore, PE was greatly increased in the outer leaflet of the plasma membrane bilayer during osteoclastogenesis, being concentrated in filopodia involved in cell-cell fusion. Immobilisation of the cell surface PE blocked osteoclast fusion, revealing the importance of PE abundance and distribution. To identify the molecules responsible for these PE dynamics, we screened a wide array of lipid-related genes by quantitative PCR and shRNA-mediated knockdown. Among them, a PE-biosynthetic enzyme, acyl-CoA:lysophosphatidylethanolamine acyltransferase 2 (LPEAT2), and two ATP-binding cassette (ABC) transporters, ABCB4 and ABCG1, were markedly increased during osteoclastogenesis, and their knockdown in pre-osteoclasts led to reduction in PE exposure on the cell surface and subsequent osteoclast fusion. These findings demonstrate that the PE dynamics play an essential role in osteoclast fusion, in which LPEAT2, ABCB4 and ABCG1 are key players for PE biosynthesis and redistribution. (日)
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| ○キーワード (推奨): |
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| ○誌名 (必須): | □ | Scientific Reports ([Nature Publishing Group])
(eISSN: 2045-2322)
| ○ISSN (任意): | □ | 2045-2322
ISSN: 2045-2322
(eISSN: 2045-2322) Title: Scientific reportsTitle(ISO): Sci RepPublisher: Nature Portfolio (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 7
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| ○号 (必須): | □ |
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| ○頁 (必須): | □ | 46715 46715
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2017年 4月 24日 (平成 29年 4月 24日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1038/srep46715 (→Scopusで検索)
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| ○PMID (任意): | □ | 28436434 (→Scopusで検索)
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| ○Scopus (任意): | □ | 2-s2.0-85038817184
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| ○機関リポジトリ : | □ | 112352
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| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1038/srep46715 (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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