| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○審査 (推奨): |
| ○カテゴリ (推奨): |
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | 佐藤 紀
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| 2. | 谷口 貴子
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| 3. | 合田 有一郎
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| 4. | 小坂 浩史
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| 5. | (英) Higashino Kosaku (日) (読)
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| 6. | 酒井 紀典 ([徳島大学.病院.中央診療施設等.高度先進整形外科診療部])
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| 7. | 加藤 真介
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| 8. | 安井 夏生
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| 9. | 西良 浩一 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.外科系.運動機能外科学])
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| 10. | 谷口 寿章
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| ○題名 (必須): | □ | (英) Proteomic Analysis of Human Tendon and Ligament: Solubilization and Analysis of Insoluble Extracellular Matrix in Connective Tissues. (日)
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| ○要約 (任意): | □ | (英) Connective tissues such as tendon, ligament and cartilage are mostly composed of extracellular matrix (ECM). These tissues are insoluble, mainly due to the highly cross-linked ECM proteins such as collagens. Difficulties obtaining suitable samples for mass spectrometric analysis render the application of modern proteomic technologies difficult. Complete solubilization of them would not only elucidate protein composition of normal tissues but also reveal pathophysiology of pathological tissues. Here we report complete solubilization of human Achilles tendon and yellow ligament, which is achieved by chemical digestion combined with successive protease treatment including elastase. The digestion mixture was subjected to liquid chromatography-mass spectrometry. The low specificity of elastase was overcome by accurate mass analysis achieved using FT-ICR-MS. In addition to the detailed proteome of both tissues, we also quantitatively determine the major protein composition of samples, by measuring peak area of some characteristic peptides detected in tissue samples and in purified proteins. As a result, differences between human Achilles tendon and yellow ligament were elucidated at molecular level. (日)
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| ○キーワード (推奨): |
| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Journal of Proteome Research ([アメリカ化学会])
(pISSN: 1535-3893, eISSN: 1535-3907)
| ○ISSN (任意): | □ | 1535-3907
ISSN: 1535-3893
(pISSN: 1535-3893, eISSN: 1535-3907) Title: Journal of proteome researchTitle(ISO): J Proteome ResPublisher: American Chemical Society (NLM Catalog)
(Scopus)
(CrossRef)
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| ○巻 (必須): | □ | 15
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| ○号 (必須): | □ | 12
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| ○頁 (必須): | □ | 4709 4721
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| ○年月日 (必須): | □ | 西暦 2016年 10月 26日 (平成 28年 10月 26日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1021/acs.jproteome.6b00806 (→Scopusで検索)
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| ○PMID (任意): | □ | 27748110 (→Scopusで検索)
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| ○備考 (任意): | 1. | (英) PublicationType: Journal Article (日)
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