| ○種別 (必須): | □ | 学術論文 (審査論文)
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| ○言語 (必須): | □ | 英語
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| ○招待 (推奨): |
| ○審査 (推奨): |
| ○カテゴリ (推奨): |
| ○共著種別 (推奨): | □ | 徳島大学以外での国内共著 (徳島大学以外でのある一つの国に属する複数機関による共同研究 (国外研究者を含まない))
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| ○学究種別 (推奨): |
| ○組織 (推奨): |
| ○著者 (必須): | 1. | (英) Shirai, H (日) (読)
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| 2. | (英) Matsushita, K (日) (読)
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| 3. | (英) Kuwahara, A (日) (読)
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| 4. | 米村 重信 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.生理系.細胞生物学])
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| 5. | (英) Nakano, T (日) (読)
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| [継承] |
| 6. | (英) Assawachananount, J (日) (読)
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| 7. | (英) Kimura, T (日) (読)
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| 8. | (英) Saito, K (日) (読)
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| 9. | (英) Terasaki, H (日) (読)
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| [継承] |
| 10. | (英) Eiraku, M (日) (読)
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| 11. | (英) Sarai, Y (日) (読)
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| [継承] |
| 12. | (英) Takahashi, Y (日) (読)
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| ○題名 (必須): | □ | (英) Transplantation of human embryonic stem cell-derived retinal tissue in two primate models of retinal degeneration. (日)
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| ○要約 (任意): | □ | (英) Retinal transplantation therapy for retinitis pigmentosa is increasingly of interest due to accumulating evidence of transplantation efficacy from animal studies and development of techniques for the differentiation of human embryonic stem cells (hESCs) and induced pluripotent stem cells into retinal tissues or cells. In this study, we aimed to assess the potential clinical utility of hESC-derived retinal tissues (hESC-retina) using newly developed primate models of retinal degeneration to obtain preparatory information regarding the potential clinical utility of these hESC-retinas in transplantation therapy. hESC-retinas were first transplanted subretinally into nude rats with or without retinal degeneration to confirm their competency as a graft to mature to form highly specified outer segment structure and to integrate after transplantation. Two focal selective photoreceptor degeneration models were then developed in monkeys by subretinal injection of cobalt chloride or 577-nm optically pumped semiconductor laser photocoagulation. The utility of the developed models and a practicality of visual acuity test developed for monkeys were evaluated. Finally, feasibility of hESC-retina transplantation was assessed in the developed monkey models under practical surgical procedure and postoperational examinations. Grafted hESC-retina was observed differentiating into a range of retinal cell types, including rod and cone photoreceptors that developed structured outer nuclear layers after transplantation. Further, immunohistochemical analyses suggested the formation of host-graft synaptic connections. The findings of this study demonstrate the clinical feasibility of hESC-retina transplantation and provide the practical tools for the optimization of transplantation strategies for future clinical applications. (日)
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| ○キーワード (推奨): | 1. | (英) Animals (日) (読)
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| 2. | (英) Cell Differentiation (日) (読)
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| 3. | (英) Cobalt (日) (読)
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| 4. | (英) Disease Models, Animal (日) (読)
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| 5. | (英) Haplorhini (日) (読)
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| 6. | (英) Human Embryonic Stem Cells (日) (読)
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| 7. | (英) Humans (日) (読)
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| 8. | (英) Photoreceptor Cells (日) (読)
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| 9. | (英) Primates (日) (読)
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| 10. | (英) Rats (日) (読)
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| 11. | (英) Retina (日) (読)
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| 12. | (英) Retinal Degeneration (日) (読)
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| ○発行所 (推奨): |
| ○誌名 (必須): | □ | Proceedings of the National Academy of Sciences of the United States of America ([The National Academy of Sciences of the United States of America])
(pISSN: 0027-8424, eISSN: 1091-6490)
| ○ISSN (任意): | □ | 1091-6490
ISSN: 0027-8424
(pISSN: 0027-8424, eISSN: 1091-6490) Title: Proceedings of the National Academy of Sciences of the United States of AmericaTitle(ISO): Proc Natl Acad Sci U S APublisher: National Academy of Sciences (NLM Catalog)
(Scopus)
(CrossRef)
(Scopus information is found. [need login]) | [継承] |
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| ○巻 (必須): | □ | 113
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| ○号 (必須): | □ | 1
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| ○頁 (必須): | □ | E81 E90
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| ○都市 (任意): |
| ○年月日 (必須): | □ | 西暦 2016年 1月 5日 (平成 28年 1月 5日)
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| ○URL (任意): |
| ○DOI (任意): | □ | 10.1073/pnas.1512590113 (→Scopusで検索)
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| ○PMID (任意): | □ | 26699487 (→Scopusで検索)
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| ○CRID (任意): |
| ○WOS (任意): | □ | 000367520400013
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| ○Scopus (任意): | □ | 2-s2.0-84953253310
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| ○評価値 (任意): |
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| ○指導教員 (推奨): |
| ○備考 (任意): | 1. | (英) Article.ELocationID: 10.1073/pnas.1512590113 (日)
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| 2. | (英) Article.PublicationTypeList.PublicationType: Journal Article (日)
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| 3. | (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't (日)
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| 4. | (英) OtherID: PMC4711854 (日)
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| 5. | (英) KeywordList.Keyword: human embryonic stem cells (日)
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| 6. | (英) KeywordList.Keyword: photoreceptors (日)
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| 7. | (英) KeywordList.Keyword: primate model (日)
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| 8. | (英) KeywordList.Keyword: retinal degeneration (日)
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| 9. | (英) KeywordList.Keyword: transplantation (日)
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