『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
ID: Pass:

登録内容 (EID=314700)

EID=314700EID:314700, Map:0, LastModified:2016年8月3日(水) 20:16:30, Operator:[大家 隆弘], Avail:TRUE, Censor:0, Owner:[稲垣 明浩], Read:継承, Write:継承, Delete:継承.
種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨):
学究種別 (推奨):
組織 (推奨): 1.徳島大学.大学院ヘルスバイオサイエンス研究部.発達予防医歯学部門.病態予防医学講座.生理機能学 (2004年4月1日〜2015年3月31日) [継承]
2.徳島大学.医学部.医学科.病態予防医学講座.細胞生物学分野 [継承]
著者 (必須): 1. (英) Hayashi Mikio (日) 林 美樹夫 (読) はやし みきお
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
2.稲垣 明浩 ([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.生理系.生理学])
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
3. (英) Ivana Novak (日) (読)
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
4. (英) Matsuda Hiroko (日) 松田 博子 (読) まつだ ひろこ
役割 (任意):
貢献度 (任意):
学籍番号 (推奨):
[継承]
題名 (必須): (英) The adenosine A2B receptor is involved in anion secretion in human pancreatic duct Capan-1 epithelial cells.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Adenosine modulates a wide variety of biological processes via adenosine receptors. In the exocrine pancreas, adenosine regulates transepithelial anion secretion in duct cells and is considered to play a role in acini-to-duct signaling. To identify the functional adenosine receptors and Cl(-) channels important for anion secretion, we herein performed experiments on Capan-1, a human pancreatic duct cell line, using open-circuit Ussing chamber and gramicidin-perforated patch-clamp techniques. The luminal addition of adenosine increased the negative transepithelial potential difference (V te) in Capan-1 monolayers with a half-maximal effective concentration value of approximately 10 M, which corresponded to the value obtained on whole-cell Cl(-) currents in Capan-1 single cells. The effects of adenosine on V te, an equivalent short-circuit current (I sc), and whole-cell Cl(-) currents were inhibited by CFTRinh-172, a cystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channel inhibitor. The adenosine A2B receptor agonist, BAY 60-6583, increased I sc and whole-cell Cl(-) currents through CFTR Cl(-) channels, whereas the A2A receptor agonist, CGS 21680, had negligible effects. The A2B receptor antagonist, PSB 603, inhibited the response of I sc to adenosine. Immunohistochemical analysis showed that the A2A and A2B receptors colocalized with Ezrin in the luminal membranes of Capan-1 monolayers and in rat pancreatic ducts. Adenosine elicited the whole-cell Cl(-) currents in guinea pig duct cells. These results demonstrate that luminal adenosine regulates anion secretion by activating CFTR Cl(-) channels via adenosine A2B receptors on the luminal membranes of Capan-1 cells. The present study endorses that purinergic signaling is important in the regulation of pancreatic secretion.  (日)    [継承]
キーワード (推奨): 1.アデノシン受容体 (adenosine receptor) [継承]
2. (英) CFTR (日) (読) [継承]
3. (英) Cl channel (日) クロライドチャネル (読) くろらいどちゃねる [継承]
4. (英) Duct (日) 分泌腺導管 (読) ぶんぴせんどうかん [継承]
5. (英) Pancreas (日) 膵臓 (読) すいぞう [継承]
発行所 (推奨): Springer-Verlag [継承]
誌名 (必須): Pflügers Archiv : European Journal of Physiology ([Springer-Verlag])
(pISSN: 0031-6768, eISSN: 1432-2013)

ISSN (任意): 1432-2013
ISSN: 0031-6768 (pISSN: 0031-6768, eISSN: 1432-2013)
Title: Pflugers Archiv : European journal of physiology
Title(ISO): Pflugers Arch
Supplier: Springer Online Journal Archive
Publisher: Springer
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
[継承]
[継承]
(必須): 468 [継承]
(必須): 7 [継承]
(必須): 1171 1181 [継承]
都市 (任意):
年月日 (必須): 西暦 2016年 7月 1日 (平成 28年 7月 1日) [継承]
URL (任意):
DOI (任意): 10.1007/s00424-016-1806-9    (→Scopusで検索) [継承]
PMID (任意): 26965147    (→Scopusで検索) [継承]
CRID (任意):
WOS (任意):
Scopus (任意):
評価値 (任意):
被引用数 (任意):
指導教員 (推奨):
備考 (任意): 1.(英) Article.Affiliation: Medical Research Project, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima, 770-8503, Japan.  (日)    [継承]
2.(英) Article.PublicationTypeList.PublicationType: Journal Article  (日)    [継承]
3.(英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't  (日)    [継承]

標準的な表示

和文冊子 ● Mikio Hayashi, Akihiro Inagaki, Novak Ivana and Hiroko Matsuda : The adenosine A2B receptor is involved in anion secretion in human pancreatic duct Capan-1 epithelial cells., Pflügers Archiv : European Journal of Physiology, Vol.468, No.7, 1171-1181, 2016.
欧文冊子 ● Mikio Hayashi, Akihiro Inagaki, Novak Ivana and Hiroko Matsuda : The adenosine A2B receptor is involved in anion secretion in human pancreatic duct Capan-1 epithelial cells., Pflügers Archiv : European Journal of Physiology, Vol.468, No.7, 1171-1181, 2016.

関連情報

Number of session users = 0, LA = 0.42, Max(EID) = 388726, Max(EOID) = 1052065.