『徳島大学 教育・研究者情報データベース (EDB)』---[学外] /
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種別 (必須): 学術論文 (審査論文) [継承]
言語 (必須): 英語 [継承]
招待 (推奨):
審査 (推奨):
カテゴリ (推奨):
共著種別 (推奨): 国際共著 (徳島大学内研究者と国外研究機関所属研究者との共同研究) [継承]
学究種別 (推奨):
組織 (推奨):
著者 (必須): 1. (英) Badsha Md Bahadur (日) (読)
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2. (英) Kurata Hiroyuki (日) (読)
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3.鬼塚 正義 ([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
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4. (英) Oga Takushi (日) (読)
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5.大政 健史
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題名 (必須): (英) Metabolic analysis of antibody producing Chinese hamster ovary cell culture under different stresses conditions.  (日)    [継承]
副題 (任意):
要約 (任意): (英) Chinese hamster ovary (CHO) cells are commonly used as the host cell lines concerning their ability to produce therapeutic proteins with complex post-translational modifications. In this study, we have investigated the time course extra- and intracellular metabolome data of the CHO-K1 cell line, under a control and stress conditions. The addition of NaCl and trehalose greatly suppressed cell growth, where the maximum viable cell density of NaCl and trehalose cultures were 2.2-fold and 2.8-fold less than that of a control culture. Contrariwise, the antibody production of both the NaCl and trehalose cultures was sustained for a longer time to surpass that of the control culture. The NaCl and trehalose cultures showed relatively similar dynamics of cell growth, antibody production, and substrate/product concentrations, while they indicated different dynamics from the control culture. The principal component analysis of extra- and intracellular metabolome dynamics indicated that their dynamic behaviors were consistent with biological functions. The qualitative pattern matching classification and hierarchical clustering analyses for the intracellular metabolome identified the metabolite clusters whose dynamic behaviors depend on NaCl and trehalose. The volcano plot revealed several reporter metabolites whose dynamics greatly change between in the NaCl and trehalose cultures. The elastic net identified some critical, intracellular metabolites that are distinct between the NaCl and trehalose. While a relatively small number of intracellular metabolites related to the cell growth, glucose, glutamine, lactate and ammonium ion concentrations, the mechanism of antibody production was suggested to be very complicated or not to be explained by elastic net regression analysis.  (日)    [継承]
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誌名 (必須): Journal of Bioscience and Bioengineering ([日本生物工学会])
(pISSN: 1389-1723, eISSN: 1347-4421)

ISSN (任意): 1347-4421
ISSN: 1389-1723 (pISSN: 1389-1723, eISSN: 1347-4421)
Title: Journal of bioscience and bioengineering
Title(ISO): J Biosci Bioeng
Supplier: 公益社団法人日本生物工学会
Publisher: Elsevier BV
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年月日 (必須): 西暦 2016年 1月 20日 (平成 28年 1月 20日) [継承]
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DOI (任意): 10.1016/j.jbiosc.2015.12.013    (→Scopusで検索) [継承]
PMID (任意): 26803706    (→Scopusで検索) [継承]
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Scopus (任意): 2-s2.0-85027930962 [継承]
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備考 (任意): 1.(英) PublicationType: JOURNAL ARTICLE  (日)    [継承]

標準的な表示

和文冊子 ● Bahadur Md Badsha, Hiroyuki Kurata, Masayoshi Onitsuka, Takushi Oga and Takeshi Omasa : Metabolic analysis of antibody producing Chinese hamster ovary cell culture under different stresses conditions., Journal of Bioscience and Bioengineering, (巻), (号), (頁), 2016.
欧文冊子 ● Bahadur Md Badsha, Hiroyuki Kurata, Masayoshi Onitsuka, Takushi Oga and Takeshi Omasa : Metabolic analysis of antibody producing Chinese hamster ovary cell culture under different stresses conditions., Journal of Bioscience and Bioengineering, (巻), (号), (頁), 2016.

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